5.7 HIV

The ELISA Test

• Uses antibodies to detect a protein and the quantity

• Sensitive

ELISA (Enzyme-Linked Immunosorbent Assay) tests are laboratory techniques used to detect and quantify proteins, antibodies, or hormones in a sample. They utilize the specific binding of antibodies to their targets, allowing for sensitive and accurate measurements. ELISA tests are widely used in medical diagnostics, research, and quality control in various industries. The process includes immobilizing an antigen to a solid surface, adding a sample that may contain an antibody, and then detecting the bound antibody through an enzyme-linked secondary antibody. A substrate is added, resulting in a measurable signal, often colorimetric, indicating the presence and concentration of the target protein.