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EExperiments for discovery and identification of DNA as genetic material (know what finding was

associated with which experiment)

1. Griffith’s experiment

2. Hershey-Chase experiment

 Structure of DNA nucleotides

 Three-dimensional structure of DNA

 Experiment to determine mode of DNA replication – Meselson and Stahl experiment

 Process of DNA replication

 Terminology: Origin of replication, replication bubble, replication fork, direction of replication

 Proteins and enzymes that play a role in DNA replication and their functions

1. Helicase

2. Single-strand binding proteins

3. Topoisomerase

4. Primase

5. DNA polymerase III

6. DNA polymerase I

7. DNA ligase

 Differences between leading strand and lagging strand synthesis

 Direction of DNA replication

 Replication of ends of DNA: Telomeres and telomerase

 DNA packaging terminology: nucleoid, chromatin, histone proteins, nucleosome, euchromatin and

heterochromatin