Anatomy and Physiology

Anatomy and Physiology

  • Anatomy – the study of the structure and form of the body
  • Physiology – the study of the functioning of the body
  • Form and function are connected; structure determines function and vice versa
  • Note: anatomical directional terms are used to describe the locations of structures

Levels of Organization

  • Atom → Molecule → Cell → Tissue → Organ → Organ System → Organism
  • Note: each level is a group of the prior level (e.g., a tissue is a group of cells)

Cells

  • All living things are made of one or more cells
  • Cells are the fundamental unit of life
  • All cells come from the division of other cells
  • There is a diversity of cell types in the human body

Major Cellular Components

  • The Cytoplasm
    • The internal, liquid component of a cell
    • Contains subunits called organelles that perform various cellular functions
  • DNA, Chromatin, and Chromosomes
    • DNA – molecule containing the genetic information of the organism; shape is a double helix (like a twisted ladder)
    • Chromatin – a partially-condensed form of DNA (as seen within the nucleus) formed by wrapping the double helix around proteins
    • Chromosomes – the most condensed form of DNA, formed by the coiling of chromatin; only seen at times of cell division
  • The Nucleus
    • Holds the cell’s DNA in the form of chromatin
    • Controls and regulates the cell’s activities
  • Mitochondria
    • “Powerhouse” of the cell
    • Generates cellular energy in the form of an ATP molecule
    • ATP production is called cellular respiration
  • Ribosomes
    • Small structures that build proteins
    • Proteins are structural and functional molecules that are built of amino acids; examples include enzymes, hormones, fibers, etc.
  • Cytoskeleton
    • Series of rod-shaped proteins
    • Provides support for cell shape, and a framework needed for generating cell movements
  • Cell Membrane
    • Separates internal and external cellular environments
    • Transports materials into and out of cells

The Cell Cycle

  • Cell Cycle – the series of events including the growth of a cell and its division into two “daughter cells”
  • Interphase – includes the growth of a daughter cell and the replicating of the DNA
  • G1 – Cell growth
  • S – DNA replication
  • G2 – Growth and prep for cell division
  • M – Mitosis

Details of the Cell Cycle

  • A cell cycle is a series of events that takes place in a cell as it grows and divides
  • A cell spends most of its time in Interphase, where it grows, replicates chromosomes, and prepares for cell division
  • The cell then leaves Interphase, undergoes Mitosis, and completes its division
  • The resulting cells (daughter cells) each enter their own Interphase and begin a new round of the cell cycle

DNA Replication

  • Single DNA molecule splits, allowing it to be copied
  • The result is two identical DNA molecules
  • Each daughter cell will get a copy

The Cell Cycle – Mitosis

  • Mitosis – the division of a cell into two daughter cells
  • Functions of Mitosis:
    • Growth – adding cells to an organism as it grows and develops
    • Repair – replaces old and damaged cells

PMAT mnemonic (Stages of Mitosis)

  • PMAT stands for Prophase, Metaphase, Anaphase, Telophase
  • Prophase (first stage): chromosomes condense and become visible as sister chromatids; spindle fibers begin to form; nucleolus breaks down; centrioles move toward opposite poles; the nuclear envelope breaks down toward the end
  • Metaphase: duplicated chromosomes line up along the center (metaphase plate) of the cell
  • Anaphase: duplicated chromosomes split at the centromere and are pulled to opposite poles; sister chromatids separate
  • Telophase: two new nuclei form; the cytoplasm divides as the cell membrane pinches to form two daughter cells
  • Interphase (before Mitosis): DNA material is copied and the chromosomes are replicated; DNA replication occurs during S phase; centrosomes/centrioles duplicate
  • In Prophase, DNA condenses into chromosomes; nucleus breaks down; spindle fibers form; centrioles move apart; nuclear envelope breaks down

Additional Mitosis Details (illustrated concepts)

  • Two pairs of centrioles are present
  • Nucleus with chromatin, nucleolus, nuclear envelope, and plasma membrane are components involved in organizing and enclosing genetic material
  • Chromosome (two sister chromatids joined at centromere) and sister chromatids are key structures during replication and separation
  • Stages of Mitosis labeled: Interphase (Synthesis of cellular components for division, including DNA replication and centriole duplication during interphase) and Prophase (coiling of chromatin into chromosomes; nucleolus breaks down; spindle fibers form; centrioles migrate; nuclear envelope breaks down)

Metaphase, Anaphase, Telophase (Summary)

  • Metaphase – duplicated chromosomes align at the center of the cell
  • Anaphase – duplicated chromosomes split into single chromosomes and move to opposite poles
  • Telophase – two new nuclei form; cytoplasm divides, resulting in two daughter cells

Transcription & Translation

  • Purpose of transcription: to make RNA copies of genes
  • Purpose of translation: to synthesize proteins for cellular functions
  • Translation produces proteins; transcription produces RNA types: mRNA, tRNA, rRNA, and non-coding RNA
  • In prokaryotes, translation and transcription occur in the cytoplasm; in eukaryotes, translation happens on ribosomes and transcription occurs in the nucleus

How Does DNA Control a Cell?

  • Transcription: segments of DNA called genes are copied into RNA
  • Translation: the RNA molecule is used by a ribosome to carry out the building of a protein
  • Therefore, DNA directs which proteins are built and when

Applications and Real-World Relevance

  • Understanding the cell cycle is essential for studying growth, development, and cancer biology
  • Proper function of the nucleus, mitochondria, and ribosomes is critical for cellular health and energy management
  • The central dogma (DNA → RNA → Protein) underpins genetics, biotechnology, and medicine
  • Knowledge of transcription and translation informs gene expression studies and therapeutic strategies
  • Ethical and practical implications arise when manipulating genetic material and targeting cell cycle processes in medical treatments