3, Semen quality assessment

Page 1: Introduction

• Lithuanian University of Health Sciences (LSMU), Faculty of Veterinary Medicine.

• Topic: Semen Quality Assessment.

• Presenter: Dr. Neringa Sutkevičienė.

Page 2: Breeding Soundness Examination

• Steps of Examination:

  1. Visual Assessment: Evaluate nails, legs, eyes, teeth, and external reproductive organs.

  2. Measurement: Measure testicular scrotum and palpate testis and epididymis.

  3. Accessory Sex Gland Evaluation: Assess prostate and vesicular glands.

  4. Semen Collection and Evaluation: Collect semen for various tests.

Page 3: Semen Testing and Collection

• Semen Testing: Process involving the measurement and analysis of semen quality.

• Collection Methods: Varies based on laboratory settings and procedures; specific companies may provide tools and protocol for collection.

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Page 4: Sperm Quality Analyzers

• Introduction to Sperm Quality Analyzers: Equipment used to assess the quality and functionality of sperm samples.

Page 5: Mobile Machines for Semen Analysis

• Models Mentioned:

  • QwikCheck™ MQCb

  • QwikCheck™ GOLD Bull

  • QwixClas 'GOLD'

• Sample Data Entry and Analysis:

  • Includes various parameters such as volume, concentration, motility percentages, and morphology conditions.

• Example metrics include total sperm motility (59.5%) and progressive motility (40.0%).

Page 6: iSperm System

• Introduction to the iSperm Analyzer: A tool for accurately measuring semen variables.

• Analyzing Parameters:

  • Concentration is measured in millions per dose, including details on motility and volume.

  • Results are displayed alongside calculations relevant for determining the number of doses and overall quality.

Page 7: Research Laboratories and Accreditation

• Accredited Laboratory: Central laboratory for spermatology at LSMU, recognized by international standards (DIN EN ISO/IEC 17025).

• Services offered include:

  • Standard and advanced sperm diagnostics

  • Compatibility, preservation, and toxicity tests.

• Faculty contacts include various specialists in large and small animal reproduction at LSMU.

Page 8: Advanced Diagnostic Techniques

• Techniques Used:

  • Computer-assisted semen analysis and flow cytometry to evaluate several sperm characteristics including membrane integrity and functionality.

Page 9: Visit from International Collaborators

• Summary of the Visit (May 22-25, 2023):

  • Prof. Dr. Dagmar Waberski from the University of Veterinary Medicine Hannover visited LSMU.

  • Discussions on collaborative research, internships, and student exchanges were held.

  • Professor presented a lecture on new technologies in semen quality assessments for students.

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Page 11: Sperm Quality Assessment Indicators

• Criteria for evaluating sperm quality include:

  • Mobility (progressive and non-progressive)

  • Morphological normality

  • Integrity of plasma membranes.

Page 12: Assessment Criteria for Semen

• Factors measured in sperm samples:

  • Volume, appearance, motility, density, staining techniques.

  • Additional tests for viability and presence of contaminating cells.

Page 13: Semen Sample Evaluation Protocol

• Template for Sample Evaluation:

  • Includes visual tests, individual motility, viability rates, and pathology assessments of sperm cells.

  • Sample identification and contact details for further inquiries provided.

Page 14: Key Quality Metrics for Sperm

• Important aspects of semen quality:

  • Motility

  • Sperm count

  • Morphological assessments.

Page 15: Quality Standards for Semen

• Two key Lithuanian standards for semen used in artificial insemination:

  • LST 1467: Bulls' semen quality indicators.

  • LST 1574: Boars' semen quality indicators.

• Updated standards released on November 30, 2022.