Lab Test 2

The quiz will contain questions based on Online Labs 3 and 4, including: cell cycle calculations, stages of mitosis, experimental design and interpretation of results, bacterial growth stages AND serial dilutions & dilution plating calculations from Lab 2.


Cell Cycle Length Calculations

Formula:

Time in spent in each phase (min) = (# of cells in phase ÷ total cells) × total cycle time (min)

Length of Cell Cycle:

Stages of mitosis

Stage

What Happens

Prophase

Chromosomes condense, spindle forms, nuclear envelope breaks

Metaphase

Chromosomes align in the center (metaphase plate)

Anaphase

Sister chromatids pulled apart to opposite poles

Telophase

Chromosomes decondense, new nuclei form

Cytokinesis

Cell splits into two (may overlap with telophase)

Experimental design and interpretation of results,

Bacterial growth stages

Phase

Description

Lag Phase

Bacteria adjust to environment, no growth yet

Log Phase

Rapid cell division and exponential growth

Stationary Phase

Growth slows, nutrients limited, cell death balances growth

Death Phase

Cells die due to lack of resources and buildup of waste

Serial dilutions calculations

Dilution plating calculations