Forensic Anthropology Syllabus notes Required textbook- can get online from pitt library CPB book Christensen, A. M., Passalacqua, N. V., & Bartelink

Forensic Anthropology 

Syllabus notes

  • Required textbook- can get online from pitt library

    • CPB book

      • Christensen, A. M., Passalacqua, N. V., & Bartelink, E. J. (2025). Forensic anthropology: current methods and practice, 3rd edition. Cognella.

      • Ebook ISBN: 979-8-8233-7695-2

      • Print ISBN: 979-8-8233-7526-9

8/24/26 notes

  • NO RECITATIONS THIS WEEK!!

    • Next week= full throttle

    • Recitation room: same one as the archaeology lab

  • Office hours are tentative right now

  • Archaeology class will help with some parts of this course- neolithic and archaeological remains as well as osteobiology

  • Will learn the basics of what bone is what

  • Readings that don't come out of the textbook are available on canvas

  • FINAL EXAM IS DURING FINALS WEEK: Tuesday, 12/8 2:00-3:50pm 

  • Skeletal CASTS are on reserve at Hillman just for this course

  • Wednesday: the history of forensic anthropology

  • No separate study guide for exams: will get info on the format of the exam 

    • *overviews and objectives are basically a study guide that you’ll just add on to every class

  • No attendance during lecture, will take during recitation

  • Multi-format exams, no essays

    • Lots of it are in a case-study format

  • Reading the book is not as important as coming to lecture and recitation 

  • RECITATION AND LABS ARE VERY VERY IMPORTANT!








8/26/26

Objectives

  • Define forensic anthropology

  • Understand where forensic anthropology fits under the broader umbrella of anthropology

  • Articulate the circumstances in which anthropology may assist in an investigation

  • Explain the difference in roles and responsibilities of a medical examiner and an anthropologist

  • Explain the importance of formative cases of the early history of forensic anthropology

    • Parkman Murder of 1849

    • Luetgert Case of 1897

    • Ruxton Case of 1935

Forensic Anthropology definition

  • The application of standard anthropological techniques, based on osteological (bones), taphonomic (everything that happens from the point of death to the point that the skeleton is discovered), and archaeological principles, in the recovery and assessment of human remains in medicolegal investigations 

When can forensic anthropology assist an investigation? 

  •  1. Unidentified, dismembered, or isolated carrion (unit of dead and/or decaying flesh)

    • Decomposing

    • Burned

    • Fragmented

    • Cremated

  • 2. Skeletonized material 

  • 3. Multiple fatalities

    • Airplane crashes

    • Wars 

      • May be part of international human rights (Bosnia, Iraq, Guatemala)

    • Acts of nature

  • 4. Persons of historic interest 

    • Last czar of Russia

    • Victims of the Titanic

  • 5. When whole body…

    • Radiographing of extensive injury

    • Sequence and trajectory of multiple bullet wounds

    • Determining age from bones

Who’s at a crime scene or involved in evidence analysis

  • Death investigator

  • Law enforcement

  • CSI

  • Ballistics

  • Blood spatter analyst

  • Trace evidence analyst

  • DNA expert

  • Entomologists

  • Botanist

  • Fingerprint experts

  • Media 

  • Medical Examiner

    • Soft tissue

    • Legal authority over cause of death

    • Don't really come to the scene of the death

  • Anthropologist

    • Hard tissue

    • No legal authority over cause of death

    • Overlap the work of an ME (osteology) and CSI (archaeology)

  • Coroners

    • Don't need to be a medical doctor, elected in 

Historical Background

Formative period- the first cases- all high profile- drew attention to forensic science

  1. Parkman murder of 1849 

    1. Physical evidence: Parkman was dismembered; body parts found in anatomy lab, privy, burnt head in furnace

    2. Anatomy was becoming important in U.S. medical institutions 

    3. Lots of anxiety surrounding medical schools

      1. Considered to be immoral and unethical because they dug up the bodies of marginalized communities 

  • Parkman

    • Medical physician who established a large asylum for “the insane” in Boston

    • Medical community didn’t love him, so he became a landlord to become unliked more

    • Went missing, eventually his body parts were found in Webster’s Chemistry lab

  • Webster

    • Chemistry professor at Harvard

    • Lent money from Parkman

  • Dr Jeffries Wyman 

    • Professor of anatomy at Harvard, examined the bones from the furnace

    • Testified that all the bones were from one person 

      • 30-40 pieces of cranium fragments

      • Fragments of a temporal bone

      • Coronoid process of the lower jaw

      • Right side of the lower jaw

      • A fragment of the 1st and 2nd or 3rd cervical vertebrae

      • Humerus fragment

      • Fragments of a leg bone

      • Fragments of hand and foot bones

    • Reassembled the body (with Holmes)

      • Determined age and height

      • Consistent with Parkman

  • Dr Keep

    • Dr Parkman's dentist since 1825

    • Examined the dentures retrieved from the furnace

    • Able to identify them as the set he made in 1846

  1. Luetgert Case of 1897

    1.  Physical evidence: greasy jelly, 4 pieces of bone - together no larger than a quarter, Louisa's ring

  •  Adolph and Louisa Leutgert

    • Known as the “sausage king”- very wealthy 

  • Adolph didn’t want to be married, killed his wife and put her in the sausage machine

  1. Ruxton Case- the Jigsaw Murders

    1.  Husband killed his wife Isabella and maid Mary

  • Body parts found decomposing in river by bystander 

    • Some fingertips were cut off, some eaten off by marine life 

  • *Important because it’s the first big case where photo super imposition was used 

    • Comparing pictures of a person to a skull to see if it fits 








8/31/26

Important people from the Formative Period

  • Thomas Dwight (1843-1911)

    • Anatomist

    • “Father of Forensic Anthropology”

    • Did the early work on trying to establish age estimates through stature and sex

  • T. Wingate Todd

    • Cleveland

    • 1912-1938 - 2,600 skeletons

    • Hamann-Todd Collection 

  • Robert J. Terry and Mildred Trotter

    • St. Louis, Missouri

    • 1814-1965 - 1,636 skeletons

Consolidation Period: 1939-1971

  • Wilton Krogman

    • Guide for the FBI to use for the identification of human skeletal material 

    • Guide to the identification of Human Skeletal Material

  • 1940s and 1950s 

    • WWII- too many bodies; too quickly

    • US ARMY- CILHI

      • Trotter took over as director in 1948

      • Meticulous record keeping

      • Developed means of measuring length of long bones to estimate stature

  • Korean War

    • ID lab est in Japan

    • T. Dale Stewart

    • Used bodies to determine age at death standards 

Modern Period: 1972-present

  • Est. of physical anthropology section of AAFS

    • Ellis Kerley and Clyde Snow

  • William Bass: Forensic Anthropology Data Bank

    • University of Tennessee, Knoxville

      • AKA the “body farm”

    • Deviation from standards

    • Developed computer program to track 

      • FORDISC

American Board of Forensic Anthropology: ABFA

  • 1977

Forensic anthropology journal 

  • Established 2018

Forensic anthropology and Expert Witness Testimony

  • Not often- most of the time no crime is committed

  • Most cases- determine whether human bone and, if human bone, assist in determining biological profile- help with identification 

  • Testify- most often when a crime is committed and skeletal evidence can assist in terming guilt or innocence- trauma, recovery, postmortem interval

Ethical guidelines

  • Do NOT misrepresent qualifications

  • Treat human remains, caseworkers, colleagues, and invested parties with professional and cultural respect 

    • Ex: NAGPRA

  • Follow the scientific method

  • “Do no harm”

    • A 12-year-old Rosemary disappeared in 1986- believed to be abducted

    • Skeletal remains discovered in 1994

    • Forensic anthropologists confirmed that the skeletal remains belonged to Rosemary

    • Rosemary and her dad were extremely close

    • In the course of the investigation, it was determined that Rosemary’s dad was NOT her biological father

    • Should the forensics team reveal this information to Rosemary’s dad? 

      • NO: it does NOT get shared






9/2/26

Are the remains of medicolegal (forensic) significance? 

  • Identification of the decedent

  • Investigation of unnatural or unattended deaths

  • Recent (typically within the 50 years- but there are exceptions

Is it bone?

  • The FIRST question a forensic scientist must ask at a crime scene

  • How to determine if bone

    • Macroscopic

      • Easiest way

    • Microscopic (but destructive)

    • XRF (X-ray fluorescence spectrometry)

    • Bone

      • Calcium

      • Phosphorus

      • Some iron

Tri-state crematory

  • Located in Noble, Georgia

  • 339 bodies in various states of decomposition

Clyde Snow’s list of questions

  1. Are the remains human?

  • 3 major characteristics of Hominins

    • Bipedalism

    • Nonhoning chewing

    • Large brains

  • Easy to determine if a bone is human when the joints (or epiphyses) are present

  • See “Skeletal Anatomy” section and “Bone Microstructure” section!!

  • Areas of misidentification

    • Bear paw skeleton looks very similar to a human hand 

    • Remains of a spiral ham looks like a human knee

  1. Do the remains represent a single individual or are they commingled? 

  • MNI= minimum number of individuals

  1. When did the death occur?

  • Typically, recent (most within 50yrs of the present)

  • Where we need to understand decomposition rates

  • Example of when timing becomes important: fetal remains

    • Was the fetus visible? 

    • Is there evidence of any trauma that cannot be explained by childbirth? 

  • “Trophy” remains

    • US common practice to bring home the skulls of enemies for display- WWII, vietnam, korean war

  • Anatomical skeletons

    • Efforts to repatriate these skeletons

    • Hardware can help determine its purpose 

  1. How old was the decedent? 

  2. What was the decedent’s sex?

  3. What is the decedent’s race? 

  • Note: this language is antiquated now; race is NOT biological. It’s a sociocultural construct- we will problematize this

  1. What was the decedent’s stature, body weight, and physique?

  2. Anatomical anomalies? 

  3. Cause of death?

  4. What was the manner of death?

  • Homicide? Suicide? Accident? Natural death?

Skeletal Anatomy

  • Limbs

    • More robust in nonhuman animals

    • Radius and ulna often fused

    • Tibia and fibula are often fused

    • Some nonhuman animals lack a fibula

  • Common misidentification with child bones

  • Skull morphology

    • Neurocranial morphology

    • Splanchnocranial (facial) morphology

    • Musculature less developed in humans

  • Mandibles (jaw)

    • Nonhuman mandibles are often “v-shaped”

    • Human mandibles are parabolic in shape, whereas nonhuman primates are “u-shaped”

Bone Microstructure

  • In humans, osteons are scattered and evenly spaced

  • In nonhumans, there is often osteon banding or plexiform bone

  • Tricky- bone differs considerably between species and between bones within one animal

Dating Techniques

  • Radiocarbon dating

    • Ex. modern bomb curve: 1950-1963

      • Atmospheric thermonuclear testing resulted in high levels of carbon-14 in terrestrial organisms









9/9/26

Introduction to Human Osteology

  • Osteology

    • The science that explores the biology, development, structure, function, and variation of bones

  • Human osteology

    • Average adult skeleton- 206 bones 

Connective tissue

  • Mesenchymal cell (type of stem cell)

    • Has the ability to differentiate into muscle, bone, cartilage…

  • Types of connective tissue

    • Irregular

      • Encapsulate organs, vessels, bones, and muscle

    • Regular

      • Bone

        • Strongest and least flexible of all connective tissues

      • Ligaments

        • Bone to bone or cartilage 

      • Tendons 

        • Muscle to bone

      • Aponeroses 

      • Fascia

        • Encases muscles, groups of muscles, and large vessels and nerves “plastic wrap”

    • Elastic 

Bone functions

  • Bone

    • Is NOT static

    • Is NOT simple

    • Is a highly complex organ system

    • Bone turnover, growth, repair, and disease response

    • Influences immune system, hormones, and gut microbiome 

  • Protection 

    • Protects the vital organs of the body- brain, heart, lungs, spinal cord

    • Houses structures essential to the senses 

  • Movement

    • Provides architecture for ligament and muscle (tendon) attachment to facilitate movement, locomotion, and joint stability

  • Mineral homeostasis and bioavailability 

    • Particularly, calcium and phosphate

    • Does NOT just store minerals

  • “Factory” for the production of blood cells

  •  Critical to the production of cells associated with the innate immune system

    • A person's first defense against invading pathogens and prevention of infection 

Bone anatomy 

  • Classification of bone by shape

    • Long bones

      • Tubular bones of the extremities

    • Flat bones

      • Are not flat

      • Form the walls of cavities

    • Irregular bones

      • Bones of the facial skeleton

      • Vertebrae

      • Carpals and tarsals

    • Sesamoid bones

      • Inside of tendons

  • Not just about shape, but differ in other ways

    • They are formed differently and grow differently

    • Differ in vascularization

    • Differ in biomechanical properties and metabolic function

    • Differ in vulnerability to pathogens and cancers 

Long bone anatomy

  • Diaphysis

    • Primary center of ossification 

  • Metaphysis

  • Epiphysis- proximal/distal

    • Secondary center of ossification

  • Epiphyseal line/ cartilaginous growth plate 

  • Periosteum

    • Wax-like membrane that covers the external surface of the bone

  • Nutrient foramen

    • Large foramen that is macroscopically visible on the bone- supplies bone and bone marrow

  • compact/ cortical bone

  • cancellous/ trabecular bone

    • Looks like “spongey bone”

  • Medullary cavity

    • Found in the center of the bone- filled with bone marrow 

  • Bone marrow  

  • Bone

    • The strongest, least flexible connective tissue

      • Compact, cortical

        • Thicker in the diaphysis

        • Thinner at joints

      • Trabecular, cancellous

  • Flat bones

    • Prolific cancellous bone

    • In the flat bones of the cranium, the cancellous bone is called diploe 






9/14/26

Biological Profile of the Skeleton

  • Biological profile includes

    • Sex estimation

    • Estimation of Age

    • Estimation of Stature

  • Why Biological Profile is so important

    • Match unidentified remains with personal records

      • Includes the same information that is in a missing persons report

    • Narrows the search for unidentified individual

      • Biological profile + individualizing characteristics

  • Assessment of one biological profile will affect assessment of the other biological profiles

    • For example, need correct sex assessment in order to accurately estimate stature 

CODIS

  • Combined DNA Index System

  • Includes: 

    • DNA profiles of missing persons

    • Profiles of unidentified human remains

    • Profiles from family members 

Stature

  • How it works: estimation of living stature of the skeleton is possible because there is a relationship with individual skeletal elements and height 

  • Early stature estimates

    • Rearticulate the entire skeleton and measure- it doesn’t really work employing the old methods and RARELY do forensic anthropologists have the entire skeleton 

Full Skeleton Methods

  • The most accurate stature estimation technique is the Full Skeleton Method- when using CURRENT methodology!

  • Based on the sum of all the vertical measurements of all bones that contribute to stature and then applying a formula

  • You need:

    • Skull

    • Vertebrae

    • Femur

    • Tibia

    • Height of the ankle- talus and calcaneus 

  • Vertebral height can be estimated by averaging the height of the vertebrae above and below the missing vertebra 

Regression methods for stature estimation

  • Based on correlations between height and body segments

  • These mathematical relationships exist between single bones and living stature AND multiple bones and living stature

  • How is correlation established?- measure bones from populations with known height 

  • Need complete bones- don’t estimate height if you do not appropriate bones

  • The femur is the most accurate

    • This is the bone that contributes the most to living stature (then, lower leg; then, arms)

  • Formulae are designed for a 95% prediction interval

  • 95% of people with the same bone measurements will fall into the stature range provided by the equation

  • Of course, this 5% will not fall within this range

  • Males and females have different proportions and require different equations

  • There is also variation within and between populations- more research needed

  • There are also secular changes in relation to height

  • Not appropriate to use stature methods established in the 1800s and early 1900s

  • We expect that equations need to be revisited as secular changes occur 

  • FORDISC

    • Forensic Data Bank Discriminant Functions

Stature in…

  • Children

    • Children grow fast

    • Known living stature of a child will be obsolete very quickly

    • More research needed

  • Older Adults

    • Compression of intervertebral discs or fractures of the vertebral bodies

    • Causes kyphosis

  • Additional variables

    • Shoes, no shoes, what kind of shoes

    • Morning v. afternoon/evening height

      • Greater impact in children

    • Reported height v/ actual height 

    • Body mass of physique

      • Really problematic! 

    • Weight of cremains

      • Mostly helpful in determining improper cremation

      • On average- weight about 2500g

      • Weigh too little or weigh too much may be a problem (way too!)

      • Cremains weight (total percentage of body weight)






9/16/26

Anatomical Position 

  • Person facing forward (anteriorly) with palms forward (anterior) and thumbs lateral

  • The right and left side of the skeleton are labeled in reference to what you see. The right side is the right side of that skeleton, the left side is the left side of that skeleton 

Axial vs. Appendicular

  • Axial

    • Everything that exists in the midline of the body

    • The skull, vertebral column, rib cage, data sacrum 

  • Appendicular

    • Limbs, shoulder girdle (clavicle and scapulae) 

Planes

  • Provide us information about different segments of the body

  • Sagittal plane

    • Divides the body into right and left sides

    • Mid sagittal

      • Right down the center of the body

  • Transverse plane

    • Divides the body into an upper part and lower part 

    • Referenced as the superior portion and interior portion 

  • Coronal plane

    • Divides the body into a front portion and back portion 

Superior/ inferior and cranial/caudal

  • Superior/ cranial

    • Things more towards the head

  • Inferior/ caudal

    • Things more towards the feet/tail (for animals)

Antierior/posterior and ventral/dorsal

  • Anterior/ ventral

    • The front

  • Posterior/ dorsal 

    •  The back

Medial/lateral

  • Medial

    • Toward then midline (middle) of the body

  • Lateral 

    • Away from the midline (middle) of the body

  • Intermediate

    • Between the two structures 

Proximal/distal

  • Proximal

    • Nearest point of attachment to limb or structure

  • Distal 

    • Farthest away from  attachment or origin 

Bone cells

  • Osteoblasts

    • Build bone

  • Osteoclasts 

    • Remove bone

  • Osteocytes

    • Mature bone cells

Cartilage cells

  • Chrondroblast

    • Builds cartilage

  • Chondroclast

    • Removes cartilage 

  • Chondrocyte

    • Mature cartilage cells 

Bone is a composite material

  • Part mineral, part collagen

    • Equates to rigidity and flexibility = strength

    • Lightweight (only about 30% of total body weight

  • Composite material of bone includes:

    • Inorganic mineral content (~60%)

      • Chemically, the inorganic component is called hydroxyapatite Ca₁₀(PO₄)₆(OH)₂ 

      • hydroxyapatite  is a specialized crystalline form of calcium phosphate

      • There are additional trace materials

    • Organic components (~25%)

      • Organic component of bone is called osteoid

      • Mainly collagen fibers

    • Water (~15%)

Ossification

  • Intramembranous ossification 

  • Endochrondral ossification 

  • The primary center of ossification 

    • There can only be one primary center of ossification for each bone

    • Keep in mind that some adult bones start out as multiple bones in the fetus (e.g., os coxa)

  • Secondary center of ossification 

    • Usually develop in the postnatal period 

  • Shortly after formation of the primary center of ossification, an organized region of rapid growth develops between the epiphysis and the diaphysis – epiphyseal plate 

  • The primary center of ossification expands towards the epiphysis for interstitial (or longitudinal) growth

  • As bone maturity approaches, cartilage proliferation slows and the epiphyseal plate thins

  • Bone at the epiphysis thickens and bone at the metaphysis thickens leading to epiphyseal union

  • This thickened bone leaves a scar in fused bone – epiphyseal line (can be detected on radiographs)

  • Ultimately, vascular breakthroughs are required for the bone to unite fully and this can occur: centrally, peripherally, or at multiple points

Epiphyseal Fusion

  • Morphological phases

    • No fusion 

    • Commencement of fusion

    • Advanced fusion

    • Complete fusion 

  • The growth plate is a weak spot in the bone and its resilience to withstand trauma is compromised 

    • 15% of all fractures in children involve the growth plate 

Appositional growth

  • The diaphysis must remodel in the transverse plane as it expands longitudinally

  • The result is a relatively constant ratio between bone addition and bone loss (in healthy bone!)




9/21/26

Can forensic anthropologists determine whether a skeleton is that of a man or a woman?

  • Nope, never!

  • Forensic anthropologists can sometimes estimate whether a skeleton is that of a male or female- it depends. 

Basic definitions 

  • Sex

    • Biological differences associated with reproductive organs and functions

  • Gender

    • A social identity that may either be self-ascribed or assigned by society

    • Forensic anthropologists can only assist with estimation of gender when there is material 

      • E.g. clothing, makeup, wings- but not really our preview

    • The skeletal remains themselves cannot be never used to estimate gender

    • Homicide and interpersonal violence are very high in the trans community, particularly for trans women. It is important for forensic anthropologists to be well versed on issues affecting the trans community 

Sexual Dimorphism 

  • Within a human population, there are differences in males and female forms

  • Body size, including body mass and height

    • Males are 10-15% larger in any given population, but significant overlap!

  • Fat distribution

    • Females- more in chest and thighs

    • Males- more in abdomen

    • Males burn fat more easily than females

  • Hair distribution, voice 

  • But in humans, this is very slight compared with many of our primate relatives and ancestors

  • In our close ape relatives, there is also overlap

  • In humans, there is very little sexual dimorphism 

Sex

  • Historically, and presently, biology has presented sex as binary in which there are males and females, but approximately 2% of the population is intersex

  • The basis for this determine includes

    • Genitalia (usually referencing external anatomy)

    • Presence of testes or ovaries (which produce sex hormones)

    • Levels of sex hormones

    • Chromosomes 

  • But these are not the only options

  • X, xxy, xyy, xxx, xxxx, xxxy

  • There is no Y

    • The Y chromosome is too small and does not contain enough genetic information to be compatible with life

  • **It is important to interrogate the pathologizing of people with these chromosomal differences. These are not recognized as human biological diversity according to clinical medicine 

Estimation of Sex

  • Dependent on 

    • The presence of certain bones

      • Pelvis- specifically the pubic region

      • Long bones- specifically for determination of Q- angle and carrying angle

      • Skull

  • Must be skewed in one direction on the spectrum

  • Age is a variable

  • There will always be people who are placed in the “unknown” category

  • Pelvis

  • Major bones that make up the pelvis: Os coxa

    • 3 parts

      • Ilium 

        • When you put your hands on your hips- you feel this bone 

      • Ischium

        • You sit on this bone

      • Pubis

        • In the front and center 

    • General shape differences 

      • In females: the shape is all about creating room for the fetus to grow and exit 

        • Broadness; pelvic inlet; iliac blades

  • Sacrum 

    • Shape of the sacrum influences the position of the coccyx

    • In males, the coccyx may be tucked in 

  • Sciatic notch

    • How we score things in labs

    • 1= greatest representation of female, 5 being greatest representation of male 

    • From wide to thin, 1 to 5

  • Pubic bone is by FAR the best 

  • Subpubic concavity (contour) 

  • Ventral arc

  • Medial aspect of the ischiopubic ramus

Skull

  • Differences in muscle attachments and overall robusticity (most on a scale of 1 to 5)

  • Bossing 

  • Nuchal crest

    • External occipital protuberance 

  • Mastoid process

  • Supraorbital margin 

  • Supraorbital ridge

  • Mandible 

  • Long bone metric analysis 

Age is a variable in sex

  • Subadults - morphomacroscopic assessments are often inaccurate and have a high inter- observer error rate

    • We are working on it

  • Older adults- hormonal changes in adults 50+ can older the bones of the skull and pelvis

    • Sex estimation becomes less accurate 

Human variation

  • Biological anthropologists are supposed to be human variation experts!
    Human variation does not map onto just male or female!

    • Forensic anthropologists and bioarchaeologists are currently working on methods that are more inclusive of the human variation in sex 






9/23/26

Dentition 

  • Human teeth

    • Human teeth are diphyodont- two generations of teeth

    • Deciduous teeth (sometimes called primary, milk, or baby teeth)

    • Permanent teeth (sometimes called secondary teeth)

    • Together- development and maturation of teeth takes almost the whole period before adulthood (skeletal adulthood)

  • Permanent dentition

    • 32 teeth

    • Adult humans have a dental formula of: 2.1.2.3/2.1.2.3

  • Deciduous teeth

    • 20 deciduous teeth 

    • Formula: 2.1.0.2/2.1.0.2

Aging the Child skeleton 

  • AGE is always given as a range (or greater than/less than)

  • Detention is more reliable than bone

  • When estimating age from BONES, you are estimating “skeletal age”

  • When estimating age from TEETH, you are estimating “Dental age”

  • You will not necessarily get the same age range for one individual. This may tell us something about growth disturbance 

  • Assess carefully for pathology- not all methods are suitable! 

Estimating age based on Interstitial growth

  • Longitudinal metrics

    • Based on interstitial bone growth

    • Good for estimation of age from fetal and infant skeletons

    • These are standard measurements for every skeleton

    • Additional variables to consider 

Estimating age based on Union of ossification centers 

  • Epiphyseal and Diaphyseal formation and fusion

    • Helpful in ageing all children

    • BUT did people recognize those epiphyses as epiphyses? 

  • The primary center of ossification

    • There can only be ONE primary center of ossification for each bone

    • Keep in mind that some adult bones start out as multiple bones in the fetus (e.g., os coxa)

  • Secondary center of ossification

    • Usually develop in the postnatal period

    • Also called epiphyses 

  • Union of ossification centers

    • Occipital bone

      • Squamous- laters 2-5 years

      • Basilar-lateral: 5-7 years

      • Spheno-occipital: 17-21 years

Estimation of age based on dental growth 

  • Dental development and eruption

    • Most reliable indicator of age

    • BUT if very young, we might not have teeth

  • Eruption

    • Process by which the tooth advances from the alveolar crypt to its full occlusal position in the mouth

    • Teeth develop inside of the alveolar bone

    • The crown and part of the tooth is formed prior to eruption

    • Completion of the development of the tooth root and its apex occurs after eruption commences  

  • Estimation of age from the dentition

  • Incisors

  • Canines 

  • Premolars

  • Molars






9/27/26

Wednesday: in-class review

  • Multiformat, hard copy, in person 

  • No recitations next week!

Ageing the Adult Skeleton 

  • Far more challenging to age an adult skeleton than a developing child/fetus

    • Because most of childhood there is a predictable way in which children are going to develop

  • Age determination

    • Subadults

      • Formation and union of primary and secondary ossification centers

      • Longitudinal growth

      • Dental calcification and eruption

    • Adults 

      • Dental eruption

      • Auricular surface and pubic symphysis

      • Sternal end of ribs (4th rib)

      • Bone histology (osteon density)

      • Ectocranial and endocranial suture closure (NOT USED ANYMORE)

        • Will only apply this if it’s the only thing to go on, since it’s not reliable 

  • General guidelines

    • Remember, age in fetuses, infants, and children is based on skeletal and dental development and growth

    • Ageing in adults is based on degenerative processes (With the exception of some early adults)

    • Age is always presented as range (or <, or >)

    • Accurate age estimation in adults requires a wider age range than estimates in nonadults

      • Precision is sacrificed for accuracy 

Degenerative changes and joints

  • ***When bone is under stress: bone is either destroyed or created ****

  • Young adults

    • Teeth

      • Eruption of the 3rd molars

      • 17-21 years (17-25)

    • Clavicle 

      • Medial (sternal) epiphysis of the clavicle (last to fuse)

      • Ossification of the medial epiphysis begins at puberty 

      • No medial epiphyseal flake present

      • Pre puberty 

      • 16-21 years of age

        • Medial epiphyseal flake appears

      • 24-29 years of age

        • Medial epiphysis covers the medial surface

      • 22-30 years of age 

        • Complete fusion 

      • Multiple ages represented, but all fused

        • All over the age of 30 years

    • Fusion of S1 and S2 (complete fusion = 25+ years)

      • Fusion occurs in a caudal to cranial direction

        • S4 and S5, S3 and S4= 12 years; S2 and S3= puberty 

      • There are 5 sacral vertebrae. Remember to consider sacralization of L5

  • Older adults

    • Auricular surface of ilium: three considerations:

      • 1. Morphological appearance of the auricular surface

        • Bone billowing, tiny striae, loss of transverse organization

        • Fine grained surface, coarse grained sandpaper, dense appearance

        • No porosity, microporosity, microporosity and macroporosity (greater than 1mm) 

      • 2. Bony changes at the apex

        • Sloping angle with a smooth margin; margins become irregular with bony buildup and lipping (margin may look sharp); much older individuals, variation is considerable 

      • 3. Bony activity in the retroauricular area 

        • Generally smooth; coarse texture and irregular and uneven surface; osteophytic growth at high and/or low relief (erosion and bony growth can occur at the same time)

      • 18-25: transverse billowing and very fine granularity

      • 25-30: reduction of billowing but retention of youthful appearance

      • 30-40: general loss of billowing, replacement by striae

      • 40-50: uniform, coarse granularity

      • 50-60: dense irregular surface of rugged topography

      • 60+: marginal lipping, macroporosity, irregularity 

    • Pubic symphysis

      • Morphology or the public symphyseal face

      • Texture change in pubic symphyseal face

      • Pubic symphysis rim 

      • Pubic symphyseal face

        • Morphology and texture: deep ridge and furrow system with transverse orientation; begin to fill in and become worn down (flat and smooth) with fine granularity; coarse granularity; pitting and depression of face (sometimes porosity)

      • Pubic rim formation and deterioration

        • Rim is absent until the pubic symphyseal face becomes flattened; dorsal margin delineates first; then the ventral margin (maybe); nodule develops on superior portion of face; nodule contributes to superior ventral margin; superior ventral margin meets inferior ventral margin creating the pubic rim; rim deteriorates over time

    • Sternal end of the 4th rib

      • Rim

        • Rim is smooth, uniform, and rounded; edges show scalloping; in early adulthood, the scalloping becomes smooth and takes on an arc shape; rim becomes irregular; can become U-shaped with bony projections; ossification of costochondral cartilage 

      • Pit

        • In the very young, the rib pit is billowed; shallow pit; pit deepens; pit becomes very deep with microporosity 

Additional ageing techniques 

  • Adults: ectocranial and endocranial suture closure (flat bones)

    • Rarely used anymore

    • Maybe if there is just a skull and no teeth

    • But, need to remember pathology 

  • Dental attrition

    • Rarely used anymore

    • Why?

    • Maybe for extremely broad categories BUT context is important 

  • Osteons and age- promising

    • Osteon count, osteon diameter, osteon perimeter, and osteon area have been shown to correlate with age 






Exam format

  • Worth 200 points

  • On paper, no scantrons (YAY!) 

  • 30 multiple choice questions

  • 10 matching

  • Case study questions (60 points)

    • For these types of questions, a scenario is described. This scenario is followed by questions regarding forensic analysis of skeletal remains associated with that forensic scenario. 

    • Some of these questions will be in Multiple Choice format; other questions may be answer with a word, number, phrase, or sentence. Lengthy explanations are not required 

  • Note: Identification of individual bones is not required for this exam. However, you should know bones and their landmarks that are relevant to the lecture discussions and lab exercises. For example, you need to know that the sciatic notch and the ischiopubic ramus because these are essential for sex estimation. You need to know the auricular surface of the ilium and the pubic symphysis because these are essential for aging adults. You will not need to visually identify or code them. However, you should know what a score of 5 means for the sciatic notch. You should know what an eroded pubic symphyseal face, with excess bone growth on the margins, and macroporosities tells us about the age of the individual.

Example questions

  1. ________ are a type of regular connective tissue that connect muscle to bone

    1. Epiphyses

    2. Aponeuroses

    3. Ligaments

    4. Tendons✅

  2. In long bones, the primary center of ossification is also known as the _______

    1. Metaphysis

    2. Periosteum

    3. Diaphysis✅

    4. Epipysis

  3. Which of the following does not describe macromorphoscopic traits?

    1. The assessment of the slope of the forehead

    2. The assessment of the pinched appearance of the ischiopubic ramus

    3. Measurement of maximum length of the nasal bridge✅

    4. The presence or absence of a large mastoid process

  4. Which of the following statements regarding endochrondral ossification is TRUE?

    1. During endochrondral ossification, cartilage turns into bone when an artery penetrates the cartilaginous model

      1. NOT TRUE, cartilage does not magically turn into bone

    2. Osteoblasts build bone inside of a cartilaginous model✅

    3. Endochondral ossification is the primary form of ossification found in the flat bones of the cranium

    4. Chondroclasts build a cartilaginous model in the rough shape of the diaphysis 

      1. NOT TRUE: ANY “CLASTS” DONT BUILD ANYTHING, “BLASTS” BUILD THINGS

  5. Generally speaking, the most reliable indicator of age in children is associated with the ____________

    1. Union of ossification centers

    2. Fusion of the epiphyses to the diaphysis

    3. Dental formation and eruption✅

    4. Fusion of the vertebrae 

Forensic Scenarios 

  1. Due to your status as an expert forensic anthropologist, you are called to the scene of a fatal house fire. Most of the human remains are fragmentary, burned, and commingled. One of the first steps that you take in your identification of individuals from this fire is to determine the Minimum Number of Individuals (MNI). In your initial assessment of MNI, you examine the intact remains: there are 3 femora, 6 tibiae, 4 fibulae, 2 right humeri, 4 left humeri, 4 radii, and 6 ulnae. Based on the information provided, the MNI = _____________.

    1. 4, because there are four left humeri✅

  2. One of the investigators on your team is working on the biological profile for another adult. Unlike the other skeletal remains, this person’s skeleton is largely intact. The skeleton is missing the right humerus, radius, ulna, and hand along with two vertebrae (T2 and L3).  Which of the following stature estimation techniques would be the most reliable in this case?

    1. Full skeleton method✅

      1. YOU HAVE ALL THE BONES THAT CONTRIBUTE TO HEIGHT, WITH THE EXCEPTION OF THE 2 MISSING VERTEBRAE- THEY CAN BE CALCULATED

    2. A regression equation using maximum length of the femur

    3. A regression equation using width of the pelvis

    4. A regression equation all long bones of the left upper limb and left lower limb

  3. You now begin your assessment of one of the adult skeletons. Unfortunately, due to fragmentation, there are no teeth present. So, you rely on the skeleton to provide an age estimate. The pubic symphyseal face has uniform horizontal bands of billowing bone. The margins of the face are rounded. There are no indications of osteoarthritis. No micro- or macroporosity is present. Which of the following ranges is the most likely age for this for this individual?

    1. 0-5 years old

    2. 80-95 years old

    3. 20-25 years old

    4. 50-55 years old