Leture 1 Animal Behaviour
Introduction and learning outcomes
Relevance of animal behaviour to veterinary medicine
Adopt an analytical approach using Tinbergen’s Four Questions
Provide examples of animal communication
Explain key stages in lifecycles and factors influencing their expression
Explain maintenance behaviour and how it relates to animal welfare
Define and explain all terminology used
Clinically relevant indicators and management concepts
Clinical indicators: e.g. inappetence, mobility scores, Grimace scales
Safe handling: recognise signs of aggression and how to manage them
Management tools: recognise signs of oestrus; prepare for parturition; intervene when necessary during birth
Recognise problematic behaviours: agonistic and injurious behaviour; stereotypies; separation anxiety in dogs
Relevance to animal welfare: link behaviour to welfare assessments and husbandry practices
Tinbergen’s Four Questions (Understanding animal behaviour)
1) Function (Adaptation)
Why is the behaviour performed? What fitness benefits does it confer?
Example: a behaviour increases an individual’s reproductive success or survival
2) Evolution (Phylogeny)
How did the behaviour evolve? How has natural selection shaped it?
Example: social signalling behaviours conserved across related species
3) Causation (Mechanism)
What are the underlying physiological, neural or environmental triggers?
Example: neural circuits and hormones triggering fleeing when threatened
4) Development (Ontogeny)
How does the behaviour develop across the animal’s lifetime?
Example: learning and maturing through experiences and socialization
References noted in the material: Dawkins (2023)
Note: There are discussions of different major “types” of behaviour (e.g., feeding, mating, parental, social) and how they may be modulated by context and experience
Four main types of behaviour (illustrative examples)
1) Feeding/sleeping behaviours
2) Social/formational behaviours (e.g., maternal care, social learning)
3) Reproductive/parental behaviours (e.g., oestrus signaling, parturition, maternal care)
4) Interactional/fitness-signalling behaviours (e.g., signalling for territory, dominance, or warning)
Conceptual note: behaviour can be adaptive (fitness-beneficial) or maladaptive in some contexts
Example reference: observations of observational learning in primates (e.g., orangutans) and comparisons with domestic dogs
Know what’s normal: Ethograms
Definition: a structured record of animal behaviour
Purpose: to establish a baseline of normal behaviour so deviations can be diagnosed
Data formats: can focus on a single behaviour category or a sequence of behaviours
Data representations: pictorial (photos) or written descriptions
Example: Kappel et al. (2024) Ethogram of the domestic cat
Practical use: helps educate owners about normal interactions with humans and other animals; supports welfare assessments
Animal communication (multimodal)
Visual, Olfactory, Auditory, Tactile channels
Clinical indicators can be conveyed via these modalities (e.g., lameness, pain) and inform safe handling
Olfactory signals can communicate social bonds, reproductive state, territory markers
Tactile signals convey social bonds and safety cues during handling
Auditory signals can convey clinical state and emotions
Grimace scales as a pain assessment tool across species
Evangelista et al. (2022): Grimace scales developed for human neonates and extended to about 10 mammalian species
Grimace scales are validated and reliable but can be modulated by various factors
Mogil et al. (2020) provides broader context on grimace scales and pain assessment
Visual communication and feline emotions (ethogram example)
Nicholson & O’Carroll (2021) ethogram for feline emotions (visual signals)
Key facial/eye cues: eyes, ears, tail movements; body posture
Fear signals (examples):
Eyes: wide, round, dilated pupils or eyes tightly shut
Ears: flattened to the side or back
Tail and body: tucked, crouching, head lowered; arched back
Overall body: piloerection, tense muscles
Anger signals (examples):
Pupils: oblong and dilated; direct stare
Ears: swivelling sideways; inner pinnae visible
Body: lowered and rigid; inverted L shape; tail slapping ground
Piloerection along spine and tail; arched back
Play signals (examples):
Pupils: dilated
Head and body posture: upward and forward facing; vertical or inverted U shape
Play face cues when kittens are involved
Content/relationship cues:
Content: pupils small; upright and forward-facing; relaxed
Interest: dilated pupils; upright and directed toward stimulus; ear movement
Practical interpretation: links between visual signals and emotional state; helps with welfare assessments and owner education
How animals perceive their environment (field of view and approach routes)
Important concept: understanding what signals animals may miss if you approach from a blind spot
Relevance to safe handling and husbandry, including herding and crowding situations
Common postures indicating confidence or submission:
Confident dog: head up, tail high; pay attention to inguinal area signals of dominance
Submissive cues: head lowered, tail tucked, body relaxed or withdrawn
General guidance: approach with caution when signals indicate potential fear or aggression; respect individual variation
Social signaling in dogs and breed diversity
Breed differences can affect recognition and interpretation of social signals across breeds (e.g., Husky vs German Shepherd vs King Charles Spaniel)
Suboptimal socialisation can impair inter-dog signal recognition and social communication
Visual cues observed in specific contexts (illustrative examples):
Chin resting; mounting; vulva winking (breeding-related signals observed in some interactions)
Practical implications for veterinary practice: tailor handling and communication to breed-specific tendencies and prior socialization history
Grimace scales and pain assessment (application in practice)
Grimace scales provide a rapid, non-invasive way to gauge pain across species
Benefits: objective behavioural indicators can guide analgesia decisions
Limitations: can be influenced by context, age, breed, environment, and ongoing pain conditions
Key sources: Evangelista et al. (2022); Mogil et al. (2020)
Olfactory communication in animals
Functions of olfactory signals:
Spatial boundaries: territory marking, latrine sites, resource holding
Temporal information: “traffic lights” signaling occupancy or readiness
Sexual behavior: increased urination in females during receptivity; mounting behaviors
Maternal and teat-seeking behavior
Foraging and feed selection; scent-based recognition
Flehmen response (lip curl) and the vomeronasal organ: detection of pheromonal cues
Contexts of olfactory signaling:
Territorial delineation; social and reproductive cues; maternal behaviors; anxiety-related behaviours in cats and dogs
Practical note: olfactory cues can be used in management and training; interpreting scent marks can aid welfare assessments
Flehmen response
Also called lip curl or gaping in cats
Triggered by novel olfactory markers, especially during breeding or exposure to pheromonal cues
Involves curling the upper lips and exposing the vomeronasal organ to enhance pheromone detection
Auditory communication
Contextual or referential signals: vary with context, audience, perspective
Chickens: predator alarms vary with predator and audience effect; tidbits in social feeding games
Dogs: bark content varies with context (e.g., warning vs. play)
Cats: purring occurrence and meaning depends on context
Identifiers: some vocalisations are individual-specific and can facilitate recognition (e.g., territorial crow calls in roosters; mother-offspring recognition in lambs)
Management tool: use audio playback (e.g., sow lactation grunt) to synchronize nursing and milk let-down in sows (biostimulation example)
Practical take-away: understanding vocal contexts improves welfare, reduces stress during handling, and informs husbandry practices
Domestic cat vocalisations (ethogram reference)
Tavernier et al. (2020): published an ethogram of cat vocalisations
Vocalisations express internal states and emotions; influenced by genetics and environment
Purpose: categorize calls for better welfare assessment and owner education; supports understanding of feline needs
Maternal behaviour and scent cues (illustrative note)
In some species, olfactory cues can trigger maternal behaviours (e.g., flehmen or pheromone signaling linked to maternal responses in certain species)
Example references imply broader cross-species comparisons of how scent influences maternal care and bonding
Practical implications for veterinary practice and welfare
Use ethograms to establish normal behaviours for individual patients
Recognize multimodal signals to inform safe handling and welfare assessments
Consider breed- and individual-specific differences in social signaling when presenting to clients
Apply Grimace scales where appropriate to monitor pain and analgesia needs
Use context-rich auditory and olfactory cues judiciously (e.g., sound playback or scent-mark management) to support welfare and care protocols
Connections to foundational principles and real-world relevance
Ethology as a foundation for clinical assessment and welfare science
Tinbergen’s questions provide a structured framework for interpreting behaviour in health and disease
Ethograms and normal/abnormal behavioural recognition underpin diagnostic reasoning and client education
Understanding communication channels supports safe handling, effective enrichment, and improved welfare outcomes in veterinary settings
Ethical, philosophical, and practical implications
Recognize animal sentience and the ethical obligation to minimize distress during care
Interpret behaviours with caution to avoid anthropomorphism; rely on validated ethograms and species-specific knowledge
Consider welfare implications of management practices (e.g., confinement, noise exposure, handling procedures) and strive for enrichment and humane care
Key terminology recap (glossary in brief)
Ethogram: a catalog or table of all observed behaviours of a species used in behavioural studies
Grimace scale: a pain assessment tool based on facial expressions across species
Flehmen response: a behavior where an animal curl-lips to expose the vomeronasal system to pheromones
Referentiation: context-dependent vocal signals that convey specific information about the situation
Tidbitting: a foraging/mammalian behavioural phenomenon where food-related cues influence social feeding and competition
Biostimulation: the use of biological signals (e.g., lactation-related vocalizations) to elicit a physiological response
Equations and quantitative notes (where applicable)
Fitness concept (informal):
These expressions illustrate the idea that behaviour can influence evolutionary success through multiple components of fitness; the exact form depends on species and context
Quick references and suggested readings (as cited in lecture)
Dawkins (2023) – Tinbergen’s Questions and behavioural analysis context
Evangelista et al. (2022) – Grimace scales and cross-species pain assessment
Mogil et al. (2020) – Broad discussion of grimace scales and pain research
Tavernier et al. (2020) – Cat vocalisation ethogram
Nicholson & O’Carroll (2021) – Feline emotions ethogram (visual signals)
Kappel et al. (2024) – Ethogram of the domestic cat
Summary takeaway
A comprehensive understanding of animal behaviour integrates functional, evolutionary, causal, and developmental perspectives with practical tools such as ethograms, Grimace scales, and multisensory communication analyses
This knowledge directly informs veterinary practice, welfare assessment, client education, and ethical care of animals in clinical and everyday settings