Cell Biology and Energy Transformation

Function of Cellular Structures

  • Plasma Membrane:

    • Acts as a barrier that separates the interior of the cell from the external environment.

    • Selectively permeable, allowing certain substances to pass while blocking others.

  • Lysosomes:

    • Organelles that contain digestive enzymes to break down waste materials and cellular debris.

    • Involved in processes like autophagy and apoptosis.

  • Chloroplast:

    • Site of photosynthesis in plant cells.

    • Contains chlorophyll, which captures light energy to convert carbon dioxide and water into glucose and oxygen.

  • Nucleoid:

    • Region in prokaryotic cells where the genetic material (DNA) is located.

    • Not surrounded by a membrane.

  • Cytosol (Cytoplasm):

    • Fluid component of the cytoplasm where organelles are suspended.

    • It's the site of many metabolic reactions.

  • Mitochondria:

    • Known as the powerhouse of the cell.

    • Responsible for energy production through cellular respiration.

Cell Walls

  • Cells with Cell Walls:

    • Found in plant cells, fungi, bacteria, and some archaea.

    • Composition:

    • Plant cell walls: Primarily composed of cellulose.

    • Fungi: Chitin.

    • Bacteria: Peptidoglycan.

Cellular Junctions

  • Types of Junctions:

    • Plant Cells:

    • Plasmodesmata: Channels between plant cell walls that facilitate communication and transport.

    • Animal Cells:

    • Tight Junctions: Seal neighboring cells together to prevent leakage.

    • Desmosomes: Anchoring junctions that provide mechanical stability.

    • Gap Junctions: Allow for intercellular communication through channels.

Energy Transformation Organelles

  • Chloroplasts:

    • Convert solar energy into chemical energy via photosynthesis.

  • Mitochondria:

    • Convert biochemical energy from nutrients into ATP through cellular respiration.

Plasma Membrane Function and Structure

  • Function:

    • Regulates the passage of substances into and out of the cell.

  • Membrane Structure:

    • Composed of a phospholipid bilayer with embedded proteins, cholesterol, and carbohydrates.

Transport Mechanisms

  • Passive Transport:

    • Movement of molecules across the membrane without energy expenditure.

    • Includes diffusion, osmosis, and facilitated diffusion.

  • Active Transport:

    • Movement against the concentration gradient, requiring energy (ATP).

    • Includes primary and secondary active transport.

Factors Affecting Molecular Movement

  • Size and shape of the molecule.

  • Polarity: Hydrophobic molecules pass easily, while hydrophilic ones do not.

  • Concentration gradient: A greater difference facilitates movement.

Tonicity and Cell Response to Solutions

  • Isotonic Solution:

    • Solute concentration equal inside and outside of the cell; no net movement.

  • Hypertonic Solution:

    • Higher solute concentration outside the cell; cells lose water and shrivel (Plasmolysis in plant cells, crenation in animal cells).

  • Hypotonic Solution:

    • Lower solute concentration outside the cell; cells gain water and swell (Turgor pressure in plant cells, lysis in animal cells).

Membrane Proteins

  • Role of Proteins:

    • Integral and peripheral proteins are involved in transport, signaling, and structural support.

  • Types of Integral Proteins:

    • Channels, transporters, receptors, and enzymes.

Tonicity Terms

  • Tonicity: Attributes of a solution in relation to its ability to influence cell volume.

Thermodynamics and Entropy

  • Laws of Thermodynamics:

    • 1st Law: Energy cannot be created or destroyed, only transformed.

    • 2nd Law: In any energy transfer, the total entropy of a system and its surroundings always increases.

  • Entropy:

    • Measure of disorder or randomness in a system.

ATP Structure and Reaction Coupling

  • ATP (Adenosine Triphosphate):

    • Consists of adenine, ribose sugar, and three phosphate groups.

    • Energy is released upon hydrolysis to ADP (Adenosine Diphosphate).

    • Coupling of exergonic (energy-releasing) and endergonic (energy-consuming) reactions is crucial for cellular processes.

Enzyme Activity

  • Factors Affecting Enzyme Activity:

    • Temperature, pH, substrate concentration, enzyme concentration, and presence of inhibitors.

Coenzymes and Enzyme Inhibition

  • Coenzymes:

    • Organic non-protein molecules that assist enzymes during catalysis (e.g., NAD, FAD).

  • Types of Inhibition:

    • Competitive: Inhibitor competes with the substrate for the active site.

    • Non-competitive: Inhibitor binds to a different part of the enzyme, altering its activity.

Enzyme Nomenclature

  • Enzymes are typically named based on the substrate they act on and the type of reaction they catalyze, often ending in “-ase.”

Cellular Respiration

  • Production of CO2 and H2O:

    • Occurs during the citric acid cycle and the electron transport chain.

  • Chemical Equation for Respiration:
    C<em>6H</em>12O<em>6+6O</em>2<br>ightarrow6CO<em>2+6H</em>2O+ATPC<em>6H</em>{12}O<em>6 + 6O</em>2 <br>ightarrow 6CO<em>2 + 6H</em>2O + ATP

  • Oxidation and Reduction:

    • Glucose (C6H12O6) is oxidized to CO2; O2 is reduced to H2O.

Phosphorylation Processes

  • Substrate-Level Phosphorylation:

    • Occurs in glycolysis and the citric acid cycle.

  • Oxidative Phosphorylation:

    • Takes place in the electron transport chain and is coupled with chemiosmosis.

Fermentation

  • Process of Fermentation:

    • Anaerobic pathway for energy production in the absence of oxygen.

    • Inputs: Glucose.

    • Outputs: Lactic acid (in animals) or ethanol and CO2 (in yeast).

    • Advantages: Rapid ATP production.

    • Disadvantages: Less energy per mole of glucose compared to aerobic respiration.

  • Aerobic vs Anaerobic:

    • Aerobic: Requires oxygen; produces more ATP.

    • Anaerobic: Does not require oxygen; produces less ATP.

  • How many times does the Krebs cycle turn for each glucose?

  • Know the two electron carriers associated with cellular respiration.

  • Which steps of respiration require oxygen and which do not?

  • Know what happens during each step of respiration and where each occurs.

  • What is glycolysis? Where does it occur?

  • Role of NADH in the Krebs Cycle and ETS?

Chapter 5
  • Light reaction of photosynthesis (1st stage)

    • Inputs & Outputs

    • Location

    • Light wavelengths absorbed the most? the least?

  • Calvin cycle of photosynthesis (2nd stage)

    • Inputs & Outputs

    • Stages (there are 3 - CO₂ fixation, CO₂ reduction and regeneration of RuBP)

    • Role of RuBP in C3 plants

    • Role of G3P

  • Equation for photosynthesis

  • Solar energy travels as what

  • What energy molecule is needed

  • What organisms can and cannot undergo photosynthesis

  • What is the 2% of light that photosynthesizers can use called

Possible Matching Topics:
  • Be able to match organelles or structures with their functions (plants, animals and prokaryotic cells)

  • ID organelles that belong to plants vs animals

  • ID type of cellular transport mechanisms (picture)

  • ID plasma membrane components (picture)

  • Stages of cellular respiration with their description

  • How many ATP produced during each stage

  • Mitochondria structures (picture)

  • Events of photosynthesis: 1st stage or 2nd stage?