Chloroplasts & Mitochondria Review

Chloroplast Overview

  • Site of photosynthesis; contain green pigment (chlorophyll)
  • Visible as oval green structures in plant cells
  • Work in tandem with mitochondria (respiration) to sustain plant energy balance

Chloroplast Structure

  • Bound by a double membrane (outer + inner)
  • Internal matrix: stroma (enzyme-rich, DNA, ribosomes)
  • Internal membrane system folds into thylakoids
    • Stacks of thylakoids form grana
  • Typical length ≈ 5μm5\,\mu\text{m}; diagram reference indicated 500nm500\,\text{nm} scale bar

Functional Adaptations

  • Double membrane controls metabolite exchange with cytoplasm
  • Thylakoid membranes provide large surface area for embedded photosystems & ATP synthase ➜ efficient light capture & photophosphorylation
  • Stroma contains Calvin-cycle enzymes ➜ carbon fixation
  • DNA & 70S ribosomes in stroma enable semi-autonomous protein synthesis for rapid response to light conditions

Measurement & Magnification Basics

  • Actual size =image sizemagnification= \dfrac{\text{image size}}{\text{magnification}}
  • Magnification =image sizeactual size= \dfrac{\text{image size}}{\text{actual size}}
  • Use scale bar (e.g., 500nm500\,\text{nm}) to derive either value; show clear working in exam answers

Chloroplast vs. Mitochondrion – Key Comparison

FeatureChloroplastMitochondrion
Primary functionPhotosynthesisAerobic respiration
Energy productsATP\text{ATP} & reduced NADPH\text{NADPH} (light-dep.)ATP\text{ATP} via oxidative phosphorylation
Main internal membraneThylakoid (forms grana)Cristae (folds of inner membrane)
Fluid matrixStromaMatrix
Pigments presentChlorophyll, carotenoidsNone
Carbon fixation?Yes (Calvin cycle in stroma)No
DNA & ribosomesPresent (circular DNA, 70S)Present (circular DNA, 70S)
Double membraneYesYes
Origin (endosymbiosis)Cyanobacterial ancestorα-proteobacterial ancestor

Exam Tips

  • Always label: outer membrane, inner membrane, stroma, thylakoid, granum, starch grain, lipid droplet (if present)
  • In calculations: keep units consistent (e.g., convert μm\mu\text{m} to nm\text{nm} when needed)
  • Table answers: use ruled columns, clear headings, parallel grammar