Chloroplasts & Mitochondria Review
Chloroplast Overview
- Site of photosynthesis; contain green pigment (chlorophyll)
- Visible as oval green structures in plant cells
- Work in tandem with mitochondria (respiration) to sustain plant energy balance
Chloroplast Structure
- Bound by a double membrane (outer + inner)
- Internal matrix: stroma (enzyme-rich, DNA, ribosomes)
- Internal membrane system folds into thylakoids
- Stacks of thylakoids form grana
- Typical length ≈ 5μm; diagram reference indicated 500nm scale bar
Functional Adaptations
- Double membrane controls metabolite exchange with cytoplasm
- Thylakoid membranes provide large surface area for embedded photosystems & ATP synthase ➜ efficient light capture & photophosphorylation
- Stroma contains Calvin-cycle enzymes ➜ carbon fixation
- DNA & 70S ribosomes in stroma enable semi-autonomous protein synthesis for rapid response to light conditions
Measurement & Magnification Basics
- Actual size =magnificationimage size
- Magnification =actual sizeimage size
- Use scale bar (e.g., 500nm) to derive either value; show clear working in exam answers
Chloroplast vs. Mitochondrion – Key Comparison
| Feature | Chloroplast | Mitochondrion |
|---|
| Primary function | Photosynthesis | Aerobic respiration |
| Energy products | ATP & reduced NADPH (light-dep.) | ATP via oxidative phosphorylation |
| Main internal membrane | Thylakoid (forms grana) | Cristae (folds of inner membrane) |
| Fluid matrix | Stroma | Matrix |
| Pigments present | Chlorophyll, carotenoids | None |
| Carbon fixation? | Yes (Calvin cycle in stroma) | No |
| DNA & ribosomes | Present (circular DNA, 70S) | Present (circular DNA, 70S) |
| Double membrane | Yes | Yes |
| Origin (endosymbiosis) | Cyanobacterial ancestor | α-proteobacterial ancestor |
Exam Tips
- Always label: outer membrane, inner membrane, stroma, thylakoid, granum, starch grain, lipid droplet (if present)
- In calculations: keep units consistent (e.g., convert μm to nm when needed)
- Table answers: use ruled columns, clear headings, parallel grammar