Mod 11
DNA= deoxyribonucleic acid (double helix, double stranded)
RNA = ribonucleic acid (single stranded)
Watson and Crick = were credited with discovering the shape of dna (double helix)
Roslind franklin took photo 51 helping discovery
Monomer = nucleotide
DNA = Adenine with Thymine (2 hydrogen bonds AT2)
DNA = Cytosine with Guanine (3 hydrogen bonds CG3)
RNA = Difference - Adenine with Uracil (No hydrogen bonds)
DNA Replication = for the entire GENOME. Takes place in the nucleus.
Helicase = unzips DNA and breaks hydrogen bonds
DNA polymerase = adds nucleotides to complementary strands
Leading strand = built continuously
Lagging = builds in Okazaki fragments then goes back
Ligase = glues okazaki fragments together that are created by lagging strand
Central Dogma = DNA -> RNA -> Protein (Known as protein synthesis)
DNA -> RNA = transcription (nucleus)
RNA -> Protein = translation (ribosome)
Ribosomes are in the rough er and cytoplasm
mRNA is made in nucleus pores , then is changed into tRNA to pick up amino acids in the cytoplasm and then goes to ribosomes, then amino acids are strung into protein
EXAMPLE:
5” ATTGCTAAGCCGTT 3”
DNA: TAACGATTCGGCAA
mRNA: 3” UAACGAUUCGGCAA 5”
tRNA matches with mRNA and mRNA matches with the DNA
AUG = START CODON
EXAMPLE
DNA: ATA
mRNA: UAU tRNA and DNA are similar
tRNA: AUA
Gene mutation = when there is a mistake in the code (nitrogenous bases)
Point mutation = only one nucleotide is changed
Deletion = Piece of nucleotides are missing
Insertion = Piece of nucleotides are added
Mutagen = something that causes a mutation (environment, genetic, carcinogens, etc…)
Gene Expression = process of controlling which gene is expressed (turned on or off)