Genotype Frequencies and Hardy-Weinberg Equilibrium
Study Notes on Hardy-Weinberg Equilibrium and Genotype Frequencies
Key Concepts
Hardy-Weinberg Principle: This principle explains how allele and genotype frequencies in a population remain constant from generation to generation in the absence of evolutionary influences.
Fundamental Equations
Basic Equations:
The equations related to genotype frequencies are as follows:
p + q = 1
p^2 + 2pq + q^2 = 1
Where:
p = frequency of the dominant allele
q = frequency of the recessive allele
Genotype Frequencies:
p^2 : Frequency of homozygous dominant genotype
2pq : Frequency of heterozygous genotype
q^2 : Frequency of homozygous recessive genotype
Example Calculations
Given values:
Let p = 0.4
Therefore, q = 1 - p = 1 - 0.4 = 0.6
Genotype Frequencies Calculation
Calculate q^2 :
q^2 = (0.6)^2 = 0.36
Calculate 2pq :
2pq = 2 imes 0.4 imes 0.6 = 0.48
Calculate p^2 :
p^2 = (0.4)^2 = 0.16
Total Frequency Check
Check the equation:
p^2 + 2pq + q^2 = 0.16 + 0.48 + 0.36 = 1
This confirms that the genotype frequencies are correct as they sum to 1.
Predictions in a Population
If the population size (N) is 300 individuals:
Homozygous Dominant (A1A1):
Frequency is given by p^2 :
0.16 imes 300 = 48
Heterozygous (A1A2):
Frequency is given by 2pq :
0.48 imes 300 = 144
Homozygous Recessive (A2A2):
Frequency is given by q^2 :
0.36 imes 300 = 108
Discrepancies and Influencing Factors
Observed Values vs. Expected:
Expected homozygous dominant individuals: 48
Observed individuals: 120
Factors Affecting Deviations
Non-random Mating:
Could lead to a reduction in heterozygotes in the population over time.
Natural Selection:
Selection may favor certain genotypes over others, affecting population dynamics.
Gene Flow:
Introduction of new individuals into the population can change allele frequencies.
Additional Calculations
For new population dynamics:
If p = 0.35 and q = 0.65 :
Calculate 2pq :
2pq = 2 imes 0.35 imes 0.65 = 0.455
For a population size of 330:
Expected number of heterozygotes = 0.455 imes 330 = 150.15
Rounding Rules:
Round genotype frequencies to three decimal places.
Round individual counts to the nearest whole number only at the end of the calculation process.