Skeletal Muscle Notes

Skeletal Muscle

Learning Objectives

  • Understand the function of the actomyosin system and its importance for movement.
  • Know the main structural differences between skeletal, heart, and smooth muscle.
  • Comprehend how contraction in the three muscle types is controlled.
  • Appreciate proprioception and know the main sensory cells involved (muscle spindles, Golgi tendon organs).

Skeletal vs Smooth vs Cardiac Muscle

FeatureSkeletalSmoothCardiac
StriationsStriated, tubular fibersNon-striated, spindle-shapedStriated, branched
NucleiMultinucleatedUninucleatedUninucleated
ControlVoluntaryInvoluntaryInvoluntary

Skeletal Muscle Structure

  • Hierarchical Organization:

    • Muscle
    • Fascicles
    • Myofibers/Myocytes
    • Myofibrils
    • Sarcomeres (basic motor unit).
  • Myofibers:

    • Multinucleated with peripheral nuclei.
    • Striated due to sarcomere arrangement within myofibrils.

Skeletal Muscle: Sarcomere

  • Components:
    • Z disc: Boundary of the sarcomere.
    • M line: Middle of the sarcomere.
    • I band: Region containing only thin filaments (actin).
    • A band: Region containing thick filaments (myosin) and overlapping thin filaments.
    • H zone: Region within the A band containing only thick filaments.
    • Thick filament: Myosin.
    • Thin filament: Actin.
    • Sarcolemma: Muscle cell membrane.
    • Sarcoplasmic reticulum: Stores calcium.
    • Transverse tubule: (T-tubule) Helps transmit action potential.
    • Mitochondrion: Provides energy for muscle contraction.

Skeletal Muscle: Sarcomere Organization

  • Z Disk: Defines the boundaries of a sarcomere.
  • M Line: Located in the middle of the sarcomere.
  • I Band: Contains only thin filaments (actin).
  • A Band: Contains thick filaments (myosin) and overlapping thin filaments.
  • Sarcolemma: The cell membrane of a muscle fiber.
  • Intermediate Filaments: E.g., Desmin. Provides structural support.

Skeletal Muscle: Connective Tissue

  • Endomysium:
    • Loose connective tissue.
    • Surrounds each muscle fiber.
    • Binds to the basement membrane of the muscle fiber.
  • Perimysium:
    • Mixed connective tissue (loose and dense).
    • Separates muscle fibers into groups called fascicles.
  • Epimysium:
    • Mostly loose connective tissue.
    • Connects the muscle body to surrounding fascia.
  • Fascia: Connective tissue layer surrounding muscles and other organs.

Skeletal Muscle: Motor Unit

  • Motor Unit (MU): A motor neuron and all the muscle fibers it innervates.
  • Innervation:
    • Each muscle fiber is innervated by only one synapse, located near the middle of the fiber.
  • Components:
    • MEP: Motor End Plate.
    • Axon Terminals: Where the motor neuron connects to the muscle fiber.
  • Neuromuscular Junction: The synapse between a motor neuron and a muscle fiber.

Contraction: Summary

  • Muscle fibers organized into fascicles contain thousands of myofibrils.
  • Sarcomeres within myofibrils contain myosin and actin filaments.
  • Motor neurons send action potentials to neuromuscular junctions, releasing Acetylcholine (ACh).
  • ACh causes depolarization at the motor end plate.
  • Action potential travels along the sarcolemma and through T-tubules to activate calcium channels in the sarcoplasmic reticulum.
  • Increased intracellular calcium levels cause a change in the troponin complex, allowing myosin to bind to actin.
  • Myosin pulls actin filaments closer using ATP, resulting in cross-bridge cycling and contraction.

Skeletal Muscle: Proprioception

  • (Alpha) α-motor neurons: Control contraction of extrafusal/regular fibers.
  • (Gamma) γ-motor neurons: Control contraction of intrafusal/spindle fibers.
  • Intrafusal (spindle) muscle fibers: Located within the muscle spindle.