Title: Alternate Methods of Phlebotomy
Context: Unit 5 - Lecture 2
Definition: Phlebotomy is the process of collecting blood for disease diagnosis.
Key Skills in Blood Collection:
Treat patients/clients courteously.
Label blood samples with patient information.
Obtain specimens successfully with minimal pain.
Refer to Lecture #1 for proper procedures with patients.
Syringe Method:
Less pressure on the vein.
Phlebotomist has more control over blood flow.
Higher cost associated with equipment.
Safety risks exist.
Winged Infusion Set (Butterfly Needle):
Expensive but associated with few safety issues.
Best for patients with fragile veins.
Capillary Puncture:
Necessary method for infants and children.
Limited blood volume collected.
Can be messy.
Required Supplies:
Syringe
Safety needle
Transfer device
Tubes required for tests ordered.
Tourniquet Application:
Slows blood flow, making veins more prominent.
Inquire about latex allergies before using latex tourniquet.
Apply for a maximum of two minutes to prevent hemoconcentration.
Locations for Venipuncture:
Median Cubital
Basilic
Cephalic
Median
Palpation Technique:
Tap with two fingers to help locate vein.
Site Preparation:
Remove the tourniquet after site selection and cleanse area.
Recommended antiseptic: 70% isopropyl alcohol.
Allow alcohol to air dry; do not touch the site again until needle insertion.
Gather and prepare all needed equipment while site is drying.
Equipment Examples Needed:
Sterile needle and syringe.
Transfer device, tubes for tests.
Open packages of sterile needle and syringe; attach needle as necessary.
Enhance plunger movement to prevent sticking.
Select proper tubes for the test.
Apply tourniquet while noting vein location.
Anchor the vein if needed; do not touch the area of needle insertion.
Hold needle at a 15-30° angle, aligned with the vein.
Remove needle cap just before puncturing with bevel facing upward.
Feel for resistance and then the "pop" as the vein is entered.
Look for "flashback" in the syringe hub to confirm entry into the vein.
Once desired amount of blood is collected:
Release the tourniquet.
Cover the needle with gauze and withdraw from the arm.
Apply pressure to the site immediately and cover the needle with a safety shield.
Attach transfer devices and fill appropriate tubes, following order of draw.
Use a transfer device to manage sample collection effectively.
Instruct patient to press on the puncture site for 2-5 minutes with arm extended.
Ensures bleeding stops and prevents hematoma formation.
Cover site with a band-aid or coban; instruct removal in 15-20 minutes.
Order of fill using transfer device:
Blood culture tubes (sterile)
Coagulation tube (light blue)
Non-additive tube (red)
Heparin tube (green)
EDTA tube (lavender or purple)
Oxalate/fluoride tube (gray)
Label tubes with initials, time, and date.
Check the patient's arm for bleeding; apply adhesive bandage/coban to the site.
Dispose of syringe and needle in a sharps container.
Clean up the area and wash hands before leaving the patient’s room.
Suitable for children and geriatric patients with fragile veins.
Needle gauges: 21-23; smaller veins require smaller gauge needles.
Needle length typically ½ to ¾ inch to accommodate lower insertion angle.
Follow similar procedures as with syringe or vacutainer methods using the wings to guide the needle into the vein.
Look for “flashback” in the tube to confirm needle placement.
Once placement is confirmed, fill required tubes using the vacutainer hub.
Complete venipuncture as previously discussed, disengaging the last tube before needle removal.