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Using Food and Controlling Growth (OCR)

B4.1 What happens during cellular respiration?

Process of Aerobic and Anaerobic Respiration

Aerobic

Anaerobic

Occurrence:

  • Occurs with oxygen and releases more energy but more slowly.

  • Occurs in most eukaryotic organisms.

Inputs:

  • Glucose (C6H12O6)

  • Oxygen (O2)

Outputs:

  • Carbon dioxide (CO2)

  • Water (H2O)

Comparative Yield of ATP:

  • 36-38 molecules per glucose molecule

Location:

  • Mitochondria

Occurrence:

  • Occurs without oxygen and releases less energy but more quickly.

  • Used by some organisms as a primary energy source or when oxygen is limited.

Inputs:

  • Glucose (C6H12O6)

Outputs: Depends on the type of anaerobic respiration;

  • Lactic acid (C3H6O3) in lactic acid fermentation

  • Ethanol (C2H5OH) and carbon dioxide (CO2) in alcoholic fermentation

Comparative Yield of ATP:

  • 2 molecules per glucose molecule

Location:

  • Cytoplasm

Cellular Respiration

  • An exothermic process where the breaking of carbon-hydrogen covalent bonds within sugars releases energy into the environment.

    • It is where organisms combine oxygen with foodstuff molecules, diverting the chemical energy in these substances into life-sustaining activities and discarding (as waste products), carbon dioxide and water.

  • Occurs in the cytoplasm and mitochondria of animal and plant cells, and in the cytoplasm of microorganisms.

  • Involves many chemical reactions and makes molecules of ATP.

    • Adenosine triphosphate (ATP): the source of energy for use and storage at the cellular level.

      • Required for processes that are essential for life, including breakdown and synthesis of molecules, active transport and muscle contraction.

  • Consumers gain biomass from other organisms when they eat them. Some of this biomass is converted into molecules of glucose, the fuel for cellular respiration.

Investigating Yeast Respiration

Effect of Different Substrates on Yeast Respiration in Yeast Strain PAG5

Example of practical investigation to study the effect of different substrates on the rate of respiration in yeast strain PAG5;

Materials:

  • Yeast strain PAG5 (commercially available)

  • Glucose solution (different concentrations)

  • Other potential substrates (e.g., sucrose, fructose, maltose) - at the same concentrations as glucose solutions

  • Measuring instrument (options based on chosen method):

    • Respirometer (measures oxygen consumption)

    • CO2 sensor (measures carbon dioxide production)

    • pH meter (measures change in pH due to acid production during anaerobic respiration)

  • Flasks or test tubes

  • Thermometer

  • Timer

Procedure:

  1. Prepare yeast suspension: Activate dried yeast PAG5 according to the manufacturer's instructions. Prepare a dilute yeast suspension in distilled water.

  2. Prepare substrate solutions: Prepare different concentrations of glucose solution (e.g., 0.1%, 0.5%, 1%) and solutions of other chosen substrates at the same concentrations.

  3. Experimental Setup:

  • Respirometer method: Set up the respirometer according to the manufacturer's instructions.

    • Add a known volume of yeast suspension and a specific volume of substrate solution to the respirometer chamber.

    • Measure oxygen consumption over time. Repeat with each substrate and different glucose concentrations.

  • CO2 sensor method: Fill flasks with a known volume of yeast suspension and different substrate solutions.

    • Seal the flasks with stoppers equipped with CO2 sensors.

    • Measure CO2 production over time.

    • Repeat with each substrate and different glucose concentrations.

  • pH meter method: Fill test tubes with a known volume of yeast suspension and different substrate solutions.

    • Measure the initial pH using a pH meter. Incubate the tubes at a constant temperature (e.g., 37°C) and measure the pH at regular time intervals.

    • Repeat with each substrate and different glucose concentrations.

  1. Control Group: Include a control group with only yeast suspension and water (no substrate) to account for endogenous respiration.

  2. Data Analysis: Plot the respiration rate (oxygen consumption, CO2 production, or change in pH) against time for each substrate and concentration.

    • Compare the slopes of the curves to determine the relative rates of respiration for different substrates.

Rate Calculations for Chemical Reactions in Cellular Respiration

Example: Using the respirometer method and measuring the decrease in oxygen concentration ([O2]) over time (Δt). We can calculate the rate of oxygen consumption (v) using the following equation;

  • Equation: v = - Δ[O2] / Δt

    • Note: The negative sign indicates that oxygen is being consumed (reactant), and the rate is expressed as the change in concentration per unit time (e.g., mmol/L/s).

Relating the Rate of Oxygen Consumption to ATP Production

Example: In cellular respiration, 1 molecule of glucose consumes 6 molecules of oxygen and produces 36-38 ATP molecules. Knowing the rate of oxygen consumption (v) from the example above, we can estimate the rate of ATP production (v_ATP) using a conversion factor:

  • Equation: v_ATP = v * (36-38 ATP molecules) / (6 O2 molecules)

    • Note: This is an estimate as the actual ATP yield can vary depending on factors like the specific metabolic pathway used by the yeast strain.

B4.2 How do we know about mitochondria and other cell structures?

Electron Microscopy

  • a powerful tool that allows scientists to visualize objects much smaller than what can be seen with a traditional light microscope.

  • Unlike light microscopes (which use light to illuminate specimens), electron microscopes utilize a beam of electrons to achieve significantly higher resolution.

    • This enables us to peer deep into the intricate world of cells and their subcellular structures, revolutionizing our understanding of biology.

How Electron Microscopy Works
  1. Specimen Preparation: Biological samples are dehydrated, embedded in a resin, and sliced into incredibly thin sections using a microtome.

    • Ultrathin sections are crucial for electron penetration.

  2. Electron Beam Generation: Electrons are emitted from a cathode and accelerated to high energies.

  3. Condenser Lenses: The electron beam is focused onto the specimen using condenser lenses.

  4. Interaction with Specimen: The electrons interact with the atoms in the specimen, and some are transmitted or scattered.

  5. Image Formation: The transmitted or scattered electrons are used to create an image by detector systems like scintillators or electron-sensitive films.

2 Main Types of Electron Microscopy Used for Studying Sub-Cellular Structures
  1. Transmission Electron Microscopy (TEM): Transmits electrons through the sample, creating a high-resolution black-and-white image.

    • Allows us to visualize structures like cell membranes, organelles (mitochondria, ribosomes, endoplasmic reticulum), and viruses.

  2. Scanning Electron Microscopy (SEM): Scans a focused electron beam across the surface of the sample, generating a three-dimensional image.

    • Useful for studying the surface features of cells, tissues, and microorganisms.

Impact of Electron Microscopy on Understanding Sub-cellular Structures

The invention of electron microscopy in the 1930s marked a turning point in cell biology.

  • Visualizing Previously Unseen Structures: Electron Microscopy (EM) allowed us to see organelles like ribosomes, Golgi apparatus, and lysosomes for the first time.

    • Structures were too small and lacked sufficient contrast for visualization with light microscopes.

  • Detailed Analysis of Organelles: EM provides high-resolution images, enabling detailed analysis of organelle structure and function.

    • It is crucial for understanding processes like protein synthesis (ribosomes), energy production (mitochondria), and cellular waste disposal (lysosomes).

  • Cellular Dynamics: EM can be used to study cells in different states, revealing changes in organelle structure and interactions during cellular processes like cell division and secretion.

  • Understanding Diseases: By examining diseased cells with EM, scientists can identify abnormalities in sub-cellular structures associated with various diseases.

Cells and Subcellular Cells

Number, Size, and Scale in Cells and Subcellular Structures

Cells and their sub-cellular structures exist in a fascinating world governed by numbers, size, and scale. The following concepts are crucial in understanding them;

  • Number: Cells come in a vast array of numbers within an organism.

    • The human body is estimated to contain trillions (10¹²) of cells.

  • Size: Cells greatly vary in size.

    • A typical human nerve cell can be a meter long, while a bacterial cell might be only a few micrometres (µm) in diameter. (1 µm = 1/1,000,000 of a meter)

  • Scale: Understanding the relative sizes of cells and their components is essential.

    • For example, The nucleus may be 5-10 µm in diameter, while a ribosome (responsible for protein synthesis), is only about 20 nanometers (nm) in size. (1 nm = 1/1,000,000,000 of a meter)

Quantitative Relationships between Units

Knowing the relationships between units like meters, micrometers, and nanometers is critical for interpreting cellular dimensions.

  • 1 meter (m) = 1,000,000 micrometers (µm)

  • 1 micrometer (µm) = 1,000 nanometers (nm)

Use of Estimations

Estimations: Valuable tools in cell biology, especially when dealing with immense numbers or sizes.

  • Cell Numbers Estimation: As it is impractical to count every cell in an organism, scientists estimate cell numbers based on tissue samples and calculations.

  • Organelle Size Estimation: Scientists estimate organelle size by comparing them to known structures in electron micrographs when it is difficult to make direct measurements.

  • When is it Used?

    • Preliminary Investigations: During initial research, estimations can provide a starting point before more precise measurements are made.

    • Large-Scale Phenomena: Estimating cell numbers in complex tissues can be more feasible than exact counts.

    • Limited Resources: When sophisticated measuring equipment is unavailable, estimations offer valuable insights.

Standard Form Calculations

Standard form: (or scientific notation), allows us to efficiently write very large or small numbers used in cellular dimensions.

  • Example: The diameter of a human egg cell is about 0.1 millimeters (mm). It is expressed in standard form as:

    • 0.1 mm = 1.0 x 10-¹ mm

      • 1.0 is the coefficient, and -1 is the exponent, indicating the number of places we move the decimal point to the left (since it's a small number).

B4.3 How do organisms grow and develop?

Cell Cycle

  • A sequence of events where a single cell grows replicates its DNA, and divides to form two daughter cells.

  • It is fundamental for the growth, repair, and development of multicellular organisms.

  • Key Phases

    • Interphase: The longest stage which occupies about 90% of the cell cycle. It is when cells undergo the following processes:

      • Growth: The cell increases in size by synthesizing new proteins and organelles.

      • Replication of DNA: The cell replicates its entire genome, ensuring each daughter cell receives a complete set of genetic instructions.

      • Preparation for Mitosis: The cell prepares for division by duplicating centrosomes (organelles that orchestrate mitosis) and synthesizing proteins needed for the mitotic process.

    • Mitosis: This is the phase where the cell divides into two daughter cells. Mitosis can be further divided into five sub-phases:

      • Prophase: Chromosomes condense and become visible.

        • The nuclear envelope begins to break down.

      • Prometaphase: Spindle fibers, formed from microtubules, start to attach to chromosomes.

        • The nuclear envelope completely disappears.

      • Metaphase: Chromosomes align at the center of the cell (metaphase plate) for separation.

      • Anaphase: Sister chromatids (copies of each chromosome) are pulled apart by spindle fibers towards opposite poles of the cell.

      • Telophase: Nuclear envelopes reform around the separated chromosomes at each pole.

        • Chromosomes decondense.

Observing Mitosis with a Light Microscope

Not all stages of mitosis are readily visible with a light microscope due to the small size of chromosomes, some key features can be observed in dividing cells:

Materials
  • Light microscope

  • Prepared slide with dividing plant or animal cells

Procedure
  1. Set Up the Microscope: Focus the microscope on low power (4x objective) and then adjust the condenser and diaphragm for optimal illumination.

  2. Locate dividing cells: On high power (10x or 40x objective), scan the slide for cells undergoing mitosis.

  • Look for areas with densely stained and condensed structures, which might be chromosomes.

  1. Identify stages: Cells at different stages of mitosis may be visible. Here are some observable features:

  • Prophase - Chromosomes appear as long, thin threads.

    • Nuclear envelopes may be partially or completely absent.

  • Metaphase - Chromosomes are condensed and aligned in a single plane at the center of the cell.

  • Anaphase - Sister chromatids are separating and moving towards opposite poles.

  • Telophase - Two separate groups of chromosomes are visible at opposite ends of the cell. The nuclear envelope may be reforming around each group.

Limitations
  • Resolution: Light microscopes cannot resolve individual chromosomes due to their small size.

  • Duration: Mitosis is a rapid process, making it challenging to capture all stages in a single observation.

Cancer

  • A non-communicable disease in humans caused by changes in a person’s DNA.

  • It is a complex group of diseases characterized by uncontrolled growth and division of cells.

  • Changes cause a cell to divide many times by mitosis, which can create a tumor.

    • Mutations: (in genes), that regulate cell growth and division are a hallmark of cancer.

      • These can activate oncogenes (cancer-promoting genes) or inactivate tumor suppressor genes, leading to uncontrolled cell proliferation.

    • Epigenetic Changes: Epigenetics refers to modifications that influence gene expression without altering the DNA sequence itself.

      • In cancer, these modifications can silence tumor suppressor genes or activate oncogenes.

The Hallmarks of Uncontrolled Growth

Key Characteristics of Cancer Cells
  • Uncontrolled Proliferation: Cancer cells lose the ability to respond to signals that normally stop cell division when enough cells are present.

  • Evading Apoptosis: Apoptosis is programmed cell death, a natural process that eliminates damaged or unwanted cells.

    • Cancer cells often evade apoptosis, allowing them to survive and accumulate.

  • Sustained Angiogenesis: Angiogenesis is the formation of new blood vessels.

    • Cancer cells can promote angiogenesis to ensure their own blood supply and facilitate further growth.

  • Invasion and Metastasis: Cancer cells can invade surrounding tissues and break away from the original tumor site.

    • Detached cells can travel through the bloodstream or lymphatic system and establish new tumors (metastasis) in distant organs, which is the leading cause of death from cancer.

The Role of Meiotic Cell Division

Meiosis: A specialized cell division process that ensures the creation of gametes (sex cells) with half the number of chromosomes compared to the parent cell.

  • Gametes: A different type of cell division produced by meiosis

    • After interphase (during which the chromosome number has doubled), two meiotic divisions occur.

    • Gametes contain half the number of chromosomes found in body cells (one chromosome from each pair).

    • At fertilization, maternal and paternal chromosomes pair up, so the zygote has the normal chromosome number.

  • This halving of the chromosome number, crucial for sexual reproduction, occurs through two meiotic divisions following a single round of DNA replication.

    • Interphase: Similar to mitosis, it is the longest stage where the following processes are done;

      • The cell grows and replicates its DNA, creating two copies of each chromosome (sister chromatids).

      • Homologous chromosomes (chromosomes of the same type, one inherited from each parent) pair up with each other, a process called synapsis.

      • Crossing over occurs, where genetic material is exchanged between homologous chromosomes, creating genetic diversity in the resulting gametes.

Two (2) Meiotic Divisions

Meiosis I: Halving the Chromosome Number

The critical stage is where the chromosome number is reduced by half. It can be further divided into four sub-phases;

  • Prophase I: Chromosomes condense and homologous chromosomes undergo synapsis.

    • Crossing over takes place.

  • Metaphase I: Paired homologous chromosomes (bivalents) align at the equator of the cell.

  • Anaphase I: Independent assortment occurs.

    • Sister chromatids of each bivalent separate from each other and move towards opposite poles of the cell, randomly distributing the homologous chromosomes.

      • It is a key step in ensuring genetic diversity.

  • Telophase I and Cytokinesis I: Nuclear envelopes reform around the separated chromosomes at each pole.

    • The cell divides, resulting in two daughter cells, each containing half the number of chromosomes (one from each homologous pair). However, each chromosome still has two sister chromatids.

Meiosis II: The Separation of Sister Chromatids

Resembles a typical mitosis, but operates on the daughter cells produced from meiosis I, which already have half the chromosome number. It also occurs in four phases:

  • Prophase II and Metaphase II: Nuclear envelopes break down, and chromosomes condense further, moving towards the metaphase plate.

  • Anaphase II: Sister chromatids of each chromosome separate and move towards opposite poles of the cell.

  • Telophase II and Cytokinesis II: Nuclear envelopes reform around the separated sister chromatids at each pole.

    • The cell divides, resulting in four haploid (half the chromosome number) daughter cells, each representing a potential gamete (egg or sperm) with unique genetic information due to crossing over and independent assortment.

Importance of Meiosis

  • Genetic Diversity: The random assortment of chromosomes and crossing over during meiosis I create gametes with unique genetic combinations, allowing for variation in offspring during sexual reproduction.

  • Maintenance of Chromosome Number: The halving of chromosome number in meiosis prevents offspring from having double the number of chromosomes with each generation.

The Function of Stem Cells

Stem Cells: Unspecialized cells with the remarkable potential to develop into various specialized cell types.

  • Crucial in both embryonic development and adult tissue repair in animals.

Animal Stem Cells

Two main types of animal stem cells;

  • Embryonic Stem Cells (ESCs): (pluripotent stem cells) meaning they have the extraordinary ability to differentiate into any cell type within an organism.

    • Found in the early stages of embryonic development, ESCs are crucial for building the body's diverse tissues and organs.

  • Adult Stem Cells: (multipotent stem cells) meaning they can differentiate into a limited number of cell types specific to their tissue of origin. Examples are;

    • Hematopoietic stem cells in bone marrow give rise to various blood cell types.

    • Neural stem cells in the brain that can differentiate into new brain cells.

    • Mesenchymal stem cells in connective tissues can develop into bone, cartilage, muscle, and fat cells.

Functions of Animal Stem Cells
  • In Embryonic Development, ESCs are the foundation for building an organism.

    • They differentiate into the three primary germ layers (ectoderm, mesoderm, endoderm) that give rise to all the different tissues and organs in the body.

  • In Tissue Repair and Regeneration, Adult stem cells help replenish and repair damaged tissues throughout life.

    • Example - after an injury, stem cells in the skin can differentiate into new skin cells to heal the wound.

Plant Meristems

Meristems: Regions of rapid cell division in plants, responsible for their growth and development. The following are the two main types of meristems:

  • Apical meristems: Located at the tips of roots and shoots.

    • Responsible for primary growth, making the plant taller or longer.

  • Lateral meristems: Found within the plant body.

    • Contribute to secondary growth, increasing the plant's thickness.

    • Example - The vascular cambium that produces wood and phloem tissues.

Functions of Plant Meristems
  • In Plant Growth - The continuous cell division in meristems allows plants to grow taller (primary growth) and thicker (secondary growth).

  • In Tissue Renewal - Meristems constantly generate new cells, replacing older ones and ensuring the plant's health and function.

  • In Regeneration - In some cases, meristems can help plants regenerate lost or damaged tissues.

Cell Differentiation

  • A fundamental process in multicellular organisms.

  • The transformation of unspecialized stem cells into a diverse array of specialized cells, each with a unique structure and function.

  • This intricate process allows the formation of complex tissues and organs, giving rise to the functional organism.

Importance of Cell Differentiation
  • In Functional Tissues and Organs - It creates a "division of labor" within the body.

    • Muscle cells contract for movement, nerve cells transmit signals, and skin cells provide a barrier.

      • Specialization ensures the efficient functioning of different tissues and organs.

  • In Organismal Complexity - Without cell differentiation, an organism would be a simple blob of identical cells, unable to perform the complex tasks necessary for survival.

  • In Development and Growth - Cell differentiation is vital for embryonic development, as it allows the formation of distinct organs and tissues from a single fertilized egg.

    • Additionally, it plays a role in post-natal growth and development.

  • In Tissue Repair and Regeneration - Adult stem cells can differentiate into specialized cells to repair damaged tissues.

    • The regenerative capacity is crucial for wound healing and maintaining tissue health.

B4.4 How is plant growth controlled? (separate science only)

Plant Hormones

Plants are able to respond to their environment in different ways. These responses are controlled and coordinated by a group of plant hormones called auxins, and increase a plant’s chances of survival.

Importance of Plant Hormones
  • Coordination: Plant hormones act as long-distance signals, coordinating growth and development between different parts of the plant.

  • Environmental Response: Plant hormones allow plants to adapt to changing environmental conditions, such as light availability and gravity.

  • Regulation of Diverse Processes: From seed germination to fruit ripening, plant hormones orchestrate a wide range of physiological processes.

Auxins: One of the most well-studied plant hormones, plays a crucial role in many of these processes. It is involved in two key plant responses:

  1. Phototropism: The bending of plant shoots towards a light source.

    • Auxin is more concentrated on the shaded side of the shoot.

    • Auxin stimulates cell elongation on the shaded side.

      • As cells on the shaded side elongate faster, the shoot bends towards the light source.

  2. Gravitropism: The growth of roots downward and shoots upward in response to gravity.

    • In roots, auxin accumulates at the root tip (positive gravitropism).

      • Auxin promotes cell elongation in the root cap on the lower side, causing the root to curve downward.

    • In shoots, auxin distribution is less uniform (negative gravitropism).

      • The differential growth induced by auxin leads to upward shoot growth.

Investigation: The Role of Auxins Phototropism

To understand auxin's role in phototropism, we can design an experiment using coleoptiles (seedling sheaths) of grass species like oats or corn. These coleoptiles exhibit strong positive phototropism.

  • This experiment helps establish the involvement of auxin in the phototropic responses of plants.

  • Further investigations can explore the effects of different auxin concentrations or inhibitors on phototropic bending.

Materials
  • Oat or corn seedlings with coleoptiles

  • Opaque covers with unilateral slits to control light direction

  • Agar blocks (growth medium)

  • Ruler

  • Scalpel

  • Petri dishes

  • Timer

Procedure
  1. Germinate seeds - Grow oats or corn seeds in moist paper towels or Petri dishes until coleoptiles reach a desired length (ex., 2-3 cm).

  2. Prepare experimental groups - Divide the seedlings into several groups:

    • Control group: Exposed to uniform light from all sides.

    • Unilateral light group: Exposed to light only from one side using the covers with slits.

    • Auxin-treated group (Optional): Apply a small amount of an auxin solution (ex. indole-3-acetic acid) to the cut tip (apex) of coleoptiles in the unilateral light group before light exposure.

  3. Record initial length - Measure and record the initial length of each coleoptile.

  4. Light exposure - Expose the seedlings in their respective groups to light for a specific duration (ex. 2-4 hours).

  5. Measure final length - After light exposure, measure the final length of each coleoptile.

  6. Safety Considerations: Use gloves and proper disposal techniques when handling plant hormones.

Analysis
  • Compare the average length changes in each group.

  • Unilateral light exposure is expected to cause bending towards the light source in the control and auxin-treated groups (if used).

  • The auxin-treated group may show a more pronounced bending response compared to the control, suggesting auxin's role in stimulating cell elongation in the shaded region.

Effects of Plant Hormones

Influence of gibberellins and ethylene (ethene) various plant functions.

Gibberellins (GA): involved in breaking seed dormancy (germination) in response to water, and bolting (production of flowers in an attempt to reproduce before death) in response to cold or lack of water.

  • A large family with over 130 identified types. The following are its key effects:

    • Stem elongation: Promoted by stimulating cell division and elongation in internodes (the stem regions between nodes).

      • This can be seen in the "bolting" phenomenon of some vegetables, where rapid stem growth occurs before flowering.

    • Seed germination: GA can break seed dormancy, allowing seeds to germinate under favorable conditions.

    • Fruit development: GA can stimulate fruit growth and development in some plants.

    • Parthenocarpy: In some cases, GA can induce fruit development without fertilization (parthenocarpy), a technique used in commercial agriculture for seedless fruits.

Ethylene (C2H4): Involved in the ripening of fruit and dropping of leaves.

  • A gas molecule that is a potent plant hormone with diverse effects such as:

    • Fruit ripening: Ethylene triggers the ripening process in fruits, causing changes in color, texture, and flavor.

    • Senescence: Ethylene promotes leaf senescence (ageing) and abscission (leaf drop), allowing plants to conserve resources and minimize water loss.

    • Epinasty: Ethylene can induce epinasty, a downward bending of stems and petioles (leaf stalks) in response to stress or injury.

    • Seed germination: Ethylene can inhibit seed germination in some plants while promoting it in others.

Use Plant Hormones to Control Plant Growth

Humans can exploit these responses and others such as triggering rooting in cuttings, by using plant hormones to trigger responses that are advantageous to us.

  1. Promoting Germination and Growth

    • Gibberellins can break seed dormancy and stimulate stem elongation which is beneficial for:

      • Overcoming seed dormancy: Certain fruits and vegetables have naturally dormant seeds that require specific conditions to germinate.

        • GA application can trigger germination, leading to more uniform and predictable crop establishment.

      • Elongation in dwarf varieties: GA can be used to promote stem elongation in dwarf crop varieties, allowing for better light interception and potentially higher yields.

  2. Enhancing Fruit Development and Ripening

    • Gibberellins (in some fruits) can enhance fruit size and development.

    • Ethylene is widely used in commercial agriculture to control fruit ripening which it's employed through:

      • Uniform Ripening: Ethylene gas treatment can be used to induce uniform ripening in fruits harvested before they reach full maturity.

        • Allows for better control over the harvest and transport process.

      • Off-Season Ripening: Certain fruits can be treated with ethylene to initiate ripening even when harvested outside their natural season.

  3. Weed Control

    • Auxin herbicides: Synthetic auxin-like molecules act as herbicides by disrupting plant growth and development.

      • These can target broadleaf weeds while leaving desired crops unharmed.

  4. Preventing Premature Fruit Drop

    • Auxins - Application can help prevent premature fruit drop in some fruit trees, reducing fruit loss and improves overall yield.

  5. Delaying Leaf Senescence and Abscission

    • Cytokinins: Hormones that can delay leaf senescence (aging) and abscission (leaf drop) which can be beneficial for:

      • Maintaining the appearance of ornamental plants - Cytokinin application can keep leaves green and healthy for a longer period, enhancing the aesthetic value of ornamental plants.

      • Extending the shelf life of harvested greens - Treatment with cytokinins can help maintain the freshness and quality of leafy greens like lettuce and spinach during storage and transportation.

Important Considerations
  • Specificity: Choosing the right hormone for the desired effect is crucial.

    • Using the wrong hormone can have unintended consequences.

  • Application method: Plant hormones can be applied in various ways, including foliar sprays, soil drenches, or seed treatments.

    • The appropriate method depends on the specific hormone and desired outcome.

  • Environmental regulations: The use of some plant hormones may be subject to regulations depending on the region.

B4.5 Should we use stem cells to treat damage and disease?

Stem cells offer the potential to treat patients by replacing damaged tissues or cells. However, the benefits must be weighed against risks and ethical concerns about the use and destruction of human embryos to collect embryonic stem cells. For these reasons, the use of stem cells in research and medicine is subject to government regulation in many countries.

Benefits of Stem Cell Therapy
  • Treatment of Incurable Diseases: Stem cells have the potential to treat currently incurable diseases like Parkinson's disease, Alzheimer's disease, and spinal cord injuries.

    • By replacing damaged cells or stimulating regeneration, stem cell therapies could offer a revolutionary approach to treatment.

  • Improved Organ Transplants: The limited availability of donor organs is a major hurdle in transplantation.

    • Stem cells could be used to grow new organs or tissues, eliminating the need for organ waiting lists.

  • Drug Discovery and Testing: Stem cells can be used to create cell models of diseases, allowing for more efficient and accurate drug discovery and testing.

Risks
  • Tumor Formation: A risk that transplanted stem cells could develop into tumors if they are not properly controlled.

  • Immune Rejection: In some cases, the body's immune system may reject transplanted stem cells, especially if they are derived from another person (allogeneic stem cells).

  • Ethical Concerns: The use of embryonic stem cells raises ethical concerns about the destruction of embryos.

Ethical Issues
  • Embryonic Stem Cells: The use of embryonic stem cells raises ethical questions about the moral status of an embryo.

  • Informed Consent: Obtaining informed consent from patients undergoing stem cell therapy can be challenging, especially if the long-term risks are not fully understood.

  • Fairness and Access: Stem cell therapies could become expensive, raising concerns about equitable access for all patients.

L

Using Food and Controlling Growth (OCR)

B4.1 What happens during cellular respiration?

Process of Aerobic and Anaerobic Respiration

Aerobic

Anaerobic

Occurrence:

  • Occurs with oxygen and releases more energy but more slowly.

  • Occurs in most eukaryotic organisms.

Inputs:

  • Glucose (C6H12O6)

  • Oxygen (O2)

Outputs:

  • Carbon dioxide (CO2)

  • Water (H2O)

Comparative Yield of ATP:

  • 36-38 molecules per glucose molecule

Location:

  • Mitochondria

Occurrence:

  • Occurs without oxygen and releases less energy but more quickly.

  • Used by some organisms as a primary energy source or when oxygen is limited.

Inputs:

  • Glucose (C6H12O6)

Outputs: Depends on the type of anaerobic respiration;

  • Lactic acid (C3H6O3) in lactic acid fermentation

  • Ethanol (C2H5OH) and carbon dioxide (CO2) in alcoholic fermentation

Comparative Yield of ATP:

  • 2 molecules per glucose molecule

Location:

  • Cytoplasm

Cellular Respiration

  • An exothermic process where the breaking of carbon-hydrogen covalent bonds within sugars releases energy into the environment.

    • It is where organisms combine oxygen with foodstuff molecules, diverting the chemical energy in these substances into life-sustaining activities and discarding (as waste products), carbon dioxide and water.

  • Occurs in the cytoplasm and mitochondria of animal and plant cells, and in the cytoplasm of microorganisms.

  • Involves many chemical reactions and makes molecules of ATP.

    • Adenosine triphosphate (ATP): the source of energy for use and storage at the cellular level.

      • Required for processes that are essential for life, including breakdown and synthesis of molecules, active transport and muscle contraction.

  • Consumers gain biomass from other organisms when they eat them. Some of this biomass is converted into molecules of glucose, the fuel for cellular respiration.

Investigating Yeast Respiration

Effect of Different Substrates on Yeast Respiration in Yeast Strain PAG5

Example of practical investigation to study the effect of different substrates on the rate of respiration in yeast strain PAG5;

Materials:

  • Yeast strain PAG5 (commercially available)

  • Glucose solution (different concentrations)

  • Other potential substrates (e.g., sucrose, fructose, maltose) - at the same concentrations as glucose solutions

  • Measuring instrument (options based on chosen method):

    • Respirometer (measures oxygen consumption)

    • CO2 sensor (measures carbon dioxide production)

    • pH meter (measures change in pH due to acid production during anaerobic respiration)

  • Flasks or test tubes

  • Thermometer

  • Timer

Procedure:

  1. Prepare yeast suspension: Activate dried yeast PAG5 according to the manufacturer's instructions. Prepare a dilute yeast suspension in distilled water.

  2. Prepare substrate solutions: Prepare different concentrations of glucose solution (e.g., 0.1%, 0.5%, 1%) and solutions of other chosen substrates at the same concentrations.

  3. Experimental Setup:

  • Respirometer method: Set up the respirometer according to the manufacturer's instructions.

    • Add a known volume of yeast suspension and a specific volume of substrate solution to the respirometer chamber.

    • Measure oxygen consumption over time. Repeat with each substrate and different glucose concentrations.

  • CO2 sensor method: Fill flasks with a known volume of yeast suspension and different substrate solutions.

    • Seal the flasks with stoppers equipped with CO2 sensors.

    • Measure CO2 production over time.

    • Repeat with each substrate and different glucose concentrations.

  • pH meter method: Fill test tubes with a known volume of yeast suspension and different substrate solutions.

    • Measure the initial pH using a pH meter. Incubate the tubes at a constant temperature (e.g., 37°C) and measure the pH at regular time intervals.

    • Repeat with each substrate and different glucose concentrations.

  1. Control Group: Include a control group with only yeast suspension and water (no substrate) to account for endogenous respiration.

  2. Data Analysis: Plot the respiration rate (oxygen consumption, CO2 production, or change in pH) against time for each substrate and concentration.

    • Compare the slopes of the curves to determine the relative rates of respiration for different substrates.

Rate Calculations for Chemical Reactions in Cellular Respiration

Example: Using the respirometer method and measuring the decrease in oxygen concentration ([O2]) over time (Δt). We can calculate the rate of oxygen consumption (v) using the following equation;

  • Equation: v = - Δ[O2] / Δt

    • Note: The negative sign indicates that oxygen is being consumed (reactant), and the rate is expressed as the change in concentration per unit time (e.g., mmol/L/s).

Relating the Rate of Oxygen Consumption to ATP Production

Example: In cellular respiration, 1 molecule of glucose consumes 6 molecules of oxygen and produces 36-38 ATP molecules. Knowing the rate of oxygen consumption (v) from the example above, we can estimate the rate of ATP production (v_ATP) using a conversion factor:

  • Equation: v_ATP = v * (36-38 ATP molecules) / (6 O2 molecules)

    • Note: This is an estimate as the actual ATP yield can vary depending on factors like the specific metabolic pathway used by the yeast strain.

B4.2 How do we know about mitochondria and other cell structures?

Electron Microscopy

  • a powerful tool that allows scientists to visualize objects much smaller than what can be seen with a traditional light microscope.

  • Unlike light microscopes (which use light to illuminate specimens), electron microscopes utilize a beam of electrons to achieve significantly higher resolution.

    • This enables us to peer deep into the intricate world of cells and their subcellular structures, revolutionizing our understanding of biology.

How Electron Microscopy Works
  1. Specimen Preparation: Biological samples are dehydrated, embedded in a resin, and sliced into incredibly thin sections using a microtome.

    • Ultrathin sections are crucial for electron penetration.

  2. Electron Beam Generation: Electrons are emitted from a cathode and accelerated to high energies.

  3. Condenser Lenses: The electron beam is focused onto the specimen using condenser lenses.

  4. Interaction with Specimen: The electrons interact with the atoms in the specimen, and some are transmitted or scattered.

  5. Image Formation: The transmitted or scattered electrons are used to create an image by detector systems like scintillators or electron-sensitive films.

2 Main Types of Electron Microscopy Used for Studying Sub-Cellular Structures
  1. Transmission Electron Microscopy (TEM): Transmits electrons through the sample, creating a high-resolution black-and-white image.

    • Allows us to visualize structures like cell membranes, organelles (mitochondria, ribosomes, endoplasmic reticulum), and viruses.

  2. Scanning Electron Microscopy (SEM): Scans a focused electron beam across the surface of the sample, generating a three-dimensional image.

    • Useful for studying the surface features of cells, tissues, and microorganisms.

Impact of Electron Microscopy on Understanding Sub-cellular Structures

The invention of electron microscopy in the 1930s marked a turning point in cell biology.

  • Visualizing Previously Unseen Structures: Electron Microscopy (EM) allowed us to see organelles like ribosomes, Golgi apparatus, and lysosomes for the first time.

    • Structures were too small and lacked sufficient contrast for visualization with light microscopes.

  • Detailed Analysis of Organelles: EM provides high-resolution images, enabling detailed analysis of organelle structure and function.

    • It is crucial for understanding processes like protein synthesis (ribosomes), energy production (mitochondria), and cellular waste disposal (lysosomes).

  • Cellular Dynamics: EM can be used to study cells in different states, revealing changes in organelle structure and interactions during cellular processes like cell division and secretion.

  • Understanding Diseases: By examining diseased cells with EM, scientists can identify abnormalities in sub-cellular structures associated with various diseases.

Cells and Subcellular Cells

Number, Size, and Scale in Cells and Subcellular Structures

Cells and their sub-cellular structures exist in a fascinating world governed by numbers, size, and scale. The following concepts are crucial in understanding them;

  • Number: Cells come in a vast array of numbers within an organism.

    • The human body is estimated to contain trillions (10¹²) of cells.

  • Size: Cells greatly vary in size.

    • A typical human nerve cell can be a meter long, while a bacterial cell might be only a few micrometres (µm) in diameter. (1 µm = 1/1,000,000 of a meter)

  • Scale: Understanding the relative sizes of cells and their components is essential.

    • For example, The nucleus may be 5-10 µm in diameter, while a ribosome (responsible for protein synthesis), is only about 20 nanometers (nm) in size. (1 nm = 1/1,000,000,000 of a meter)

Quantitative Relationships between Units

Knowing the relationships between units like meters, micrometers, and nanometers is critical for interpreting cellular dimensions.

  • 1 meter (m) = 1,000,000 micrometers (µm)

  • 1 micrometer (µm) = 1,000 nanometers (nm)

Use of Estimations

Estimations: Valuable tools in cell biology, especially when dealing with immense numbers or sizes.

  • Cell Numbers Estimation: As it is impractical to count every cell in an organism, scientists estimate cell numbers based on tissue samples and calculations.

  • Organelle Size Estimation: Scientists estimate organelle size by comparing them to known structures in electron micrographs when it is difficult to make direct measurements.

  • When is it Used?

    • Preliminary Investigations: During initial research, estimations can provide a starting point before more precise measurements are made.

    • Large-Scale Phenomena: Estimating cell numbers in complex tissues can be more feasible than exact counts.

    • Limited Resources: When sophisticated measuring equipment is unavailable, estimations offer valuable insights.

Standard Form Calculations

Standard form: (or scientific notation), allows us to efficiently write very large or small numbers used in cellular dimensions.

  • Example: The diameter of a human egg cell is about 0.1 millimeters (mm). It is expressed in standard form as:

    • 0.1 mm = 1.0 x 10-¹ mm

      • 1.0 is the coefficient, and -1 is the exponent, indicating the number of places we move the decimal point to the left (since it's a small number).

B4.3 How do organisms grow and develop?

Cell Cycle

  • A sequence of events where a single cell grows replicates its DNA, and divides to form two daughter cells.

  • It is fundamental for the growth, repair, and development of multicellular organisms.

  • Key Phases

    • Interphase: The longest stage which occupies about 90% of the cell cycle. It is when cells undergo the following processes:

      • Growth: The cell increases in size by synthesizing new proteins and organelles.

      • Replication of DNA: The cell replicates its entire genome, ensuring each daughter cell receives a complete set of genetic instructions.

      • Preparation for Mitosis: The cell prepares for division by duplicating centrosomes (organelles that orchestrate mitosis) and synthesizing proteins needed for the mitotic process.

    • Mitosis: This is the phase where the cell divides into two daughter cells. Mitosis can be further divided into five sub-phases:

      • Prophase: Chromosomes condense and become visible.

        • The nuclear envelope begins to break down.

      • Prometaphase: Spindle fibers, formed from microtubules, start to attach to chromosomes.

        • The nuclear envelope completely disappears.

      • Metaphase: Chromosomes align at the center of the cell (metaphase plate) for separation.

      • Anaphase: Sister chromatids (copies of each chromosome) are pulled apart by spindle fibers towards opposite poles of the cell.

      • Telophase: Nuclear envelopes reform around the separated chromosomes at each pole.

        • Chromosomes decondense.

Observing Mitosis with a Light Microscope

Not all stages of mitosis are readily visible with a light microscope due to the small size of chromosomes, some key features can be observed in dividing cells:

Materials
  • Light microscope

  • Prepared slide with dividing plant or animal cells

Procedure
  1. Set Up the Microscope: Focus the microscope on low power (4x objective) and then adjust the condenser and diaphragm for optimal illumination.

  2. Locate dividing cells: On high power (10x or 40x objective), scan the slide for cells undergoing mitosis.

  • Look for areas with densely stained and condensed structures, which might be chromosomes.

  1. Identify stages: Cells at different stages of mitosis may be visible. Here are some observable features:

  • Prophase - Chromosomes appear as long, thin threads.

    • Nuclear envelopes may be partially or completely absent.

  • Metaphase - Chromosomes are condensed and aligned in a single plane at the center of the cell.

  • Anaphase - Sister chromatids are separating and moving towards opposite poles.

  • Telophase - Two separate groups of chromosomes are visible at opposite ends of the cell. The nuclear envelope may be reforming around each group.

Limitations
  • Resolution: Light microscopes cannot resolve individual chromosomes due to their small size.

  • Duration: Mitosis is a rapid process, making it challenging to capture all stages in a single observation.

Cancer

  • A non-communicable disease in humans caused by changes in a person’s DNA.

  • It is a complex group of diseases characterized by uncontrolled growth and division of cells.

  • Changes cause a cell to divide many times by mitosis, which can create a tumor.

    • Mutations: (in genes), that regulate cell growth and division are a hallmark of cancer.

      • These can activate oncogenes (cancer-promoting genes) or inactivate tumor suppressor genes, leading to uncontrolled cell proliferation.

    • Epigenetic Changes: Epigenetics refers to modifications that influence gene expression without altering the DNA sequence itself.

      • In cancer, these modifications can silence tumor suppressor genes or activate oncogenes.

The Hallmarks of Uncontrolled Growth

Key Characteristics of Cancer Cells
  • Uncontrolled Proliferation: Cancer cells lose the ability to respond to signals that normally stop cell division when enough cells are present.

  • Evading Apoptosis: Apoptosis is programmed cell death, a natural process that eliminates damaged or unwanted cells.

    • Cancer cells often evade apoptosis, allowing them to survive and accumulate.

  • Sustained Angiogenesis: Angiogenesis is the formation of new blood vessels.

    • Cancer cells can promote angiogenesis to ensure their own blood supply and facilitate further growth.

  • Invasion and Metastasis: Cancer cells can invade surrounding tissues and break away from the original tumor site.

    • Detached cells can travel through the bloodstream or lymphatic system and establish new tumors (metastasis) in distant organs, which is the leading cause of death from cancer.

The Role of Meiotic Cell Division

Meiosis: A specialized cell division process that ensures the creation of gametes (sex cells) with half the number of chromosomes compared to the parent cell.

  • Gametes: A different type of cell division produced by meiosis

    • After interphase (during which the chromosome number has doubled), two meiotic divisions occur.

    • Gametes contain half the number of chromosomes found in body cells (one chromosome from each pair).

    • At fertilization, maternal and paternal chromosomes pair up, so the zygote has the normal chromosome number.

  • This halving of the chromosome number, crucial for sexual reproduction, occurs through two meiotic divisions following a single round of DNA replication.

    • Interphase: Similar to mitosis, it is the longest stage where the following processes are done;

      • The cell grows and replicates its DNA, creating two copies of each chromosome (sister chromatids).

      • Homologous chromosomes (chromosomes of the same type, one inherited from each parent) pair up with each other, a process called synapsis.

      • Crossing over occurs, where genetic material is exchanged between homologous chromosomes, creating genetic diversity in the resulting gametes.

Two (2) Meiotic Divisions

Meiosis I: Halving the Chromosome Number

The critical stage is where the chromosome number is reduced by half. It can be further divided into four sub-phases;

  • Prophase I: Chromosomes condense and homologous chromosomes undergo synapsis.

    • Crossing over takes place.

  • Metaphase I: Paired homologous chromosomes (bivalents) align at the equator of the cell.

  • Anaphase I: Independent assortment occurs.

    • Sister chromatids of each bivalent separate from each other and move towards opposite poles of the cell, randomly distributing the homologous chromosomes.

      • It is a key step in ensuring genetic diversity.

  • Telophase I and Cytokinesis I: Nuclear envelopes reform around the separated chromosomes at each pole.

    • The cell divides, resulting in two daughter cells, each containing half the number of chromosomes (one from each homologous pair). However, each chromosome still has two sister chromatids.

Meiosis II: The Separation of Sister Chromatids

Resembles a typical mitosis, but operates on the daughter cells produced from meiosis I, which already have half the chromosome number. It also occurs in four phases:

  • Prophase II and Metaphase II: Nuclear envelopes break down, and chromosomes condense further, moving towards the metaphase plate.

  • Anaphase II: Sister chromatids of each chromosome separate and move towards opposite poles of the cell.

  • Telophase II and Cytokinesis II: Nuclear envelopes reform around the separated sister chromatids at each pole.

    • The cell divides, resulting in four haploid (half the chromosome number) daughter cells, each representing a potential gamete (egg or sperm) with unique genetic information due to crossing over and independent assortment.

Importance of Meiosis

  • Genetic Diversity: The random assortment of chromosomes and crossing over during meiosis I create gametes with unique genetic combinations, allowing for variation in offspring during sexual reproduction.

  • Maintenance of Chromosome Number: The halving of chromosome number in meiosis prevents offspring from having double the number of chromosomes with each generation.

The Function of Stem Cells

Stem Cells: Unspecialized cells with the remarkable potential to develop into various specialized cell types.

  • Crucial in both embryonic development and adult tissue repair in animals.

Animal Stem Cells

Two main types of animal stem cells;

  • Embryonic Stem Cells (ESCs): (pluripotent stem cells) meaning they have the extraordinary ability to differentiate into any cell type within an organism.

    • Found in the early stages of embryonic development, ESCs are crucial for building the body's diverse tissues and organs.

  • Adult Stem Cells: (multipotent stem cells) meaning they can differentiate into a limited number of cell types specific to their tissue of origin. Examples are;

    • Hematopoietic stem cells in bone marrow give rise to various blood cell types.

    • Neural stem cells in the brain that can differentiate into new brain cells.

    • Mesenchymal stem cells in connective tissues can develop into bone, cartilage, muscle, and fat cells.

Functions of Animal Stem Cells
  • In Embryonic Development, ESCs are the foundation for building an organism.

    • They differentiate into the three primary germ layers (ectoderm, mesoderm, endoderm) that give rise to all the different tissues and organs in the body.

  • In Tissue Repair and Regeneration, Adult stem cells help replenish and repair damaged tissues throughout life.

    • Example - after an injury, stem cells in the skin can differentiate into new skin cells to heal the wound.

Plant Meristems

Meristems: Regions of rapid cell division in plants, responsible for their growth and development. The following are the two main types of meristems:

  • Apical meristems: Located at the tips of roots and shoots.

    • Responsible for primary growth, making the plant taller or longer.

  • Lateral meristems: Found within the plant body.

    • Contribute to secondary growth, increasing the plant's thickness.

    • Example - The vascular cambium that produces wood and phloem tissues.

Functions of Plant Meristems
  • In Plant Growth - The continuous cell division in meristems allows plants to grow taller (primary growth) and thicker (secondary growth).

  • In Tissue Renewal - Meristems constantly generate new cells, replacing older ones and ensuring the plant's health and function.

  • In Regeneration - In some cases, meristems can help plants regenerate lost or damaged tissues.

Cell Differentiation

  • A fundamental process in multicellular organisms.

  • The transformation of unspecialized stem cells into a diverse array of specialized cells, each with a unique structure and function.

  • This intricate process allows the formation of complex tissues and organs, giving rise to the functional organism.

Importance of Cell Differentiation
  • In Functional Tissues and Organs - It creates a "division of labor" within the body.

    • Muscle cells contract for movement, nerve cells transmit signals, and skin cells provide a barrier.

      • Specialization ensures the efficient functioning of different tissues and organs.

  • In Organismal Complexity - Without cell differentiation, an organism would be a simple blob of identical cells, unable to perform the complex tasks necessary for survival.

  • In Development and Growth - Cell differentiation is vital for embryonic development, as it allows the formation of distinct organs and tissues from a single fertilized egg.

    • Additionally, it plays a role in post-natal growth and development.

  • In Tissue Repair and Regeneration - Adult stem cells can differentiate into specialized cells to repair damaged tissues.

    • The regenerative capacity is crucial for wound healing and maintaining tissue health.

B4.4 How is plant growth controlled? (separate science only)

Plant Hormones

Plants are able to respond to their environment in different ways. These responses are controlled and coordinated by a group of plant hormones called auxins, and increase a plant’s chances of survival.

Importance of Plant Hormones
  • Coordination: Plant hormones act as long-distance signals, coordinating growth and development between different parts of the plant.

  • Environmental Response: Plant hormones allow plants to adapt to changing environmental conditions, such as light availability and gravity.

  • Regulation of Diverse Processes: From seed germination to fruit ripening, plant hormones orchestrate a wide range of physiological processes.

Auxins: One of the most well-studied plant hormones, plays a crucial role in many of these processes. It is involved in two key plant responses:

  1. Phototropism: The bending of plant shoots towards a light source.

    • Auxin is more concentrated on the shaded side of the shoot.

    • Auxin stimulates cell elongation on the shaded side.

      • As cells on the shaded side elongate faster, the shoot bends towards the light source.

  2. Gravitropism: The growth of roots downward and shoots upward in response to gravity.

    • In roots, auxin accumulates at the root tip (positive gravitropism).

      • Auxin promotes cell elongation in the root cap on the lower side, causing the root to curve downward.

    • In shoots, auxin distribution is less uniform (negative gravitropism).

      • The differential growth induced by auxin leads to upward shoot growth.

Investigation: The Role of Auxins Phototropism

To understand auxin's role in phototropism, we can design an experiment using coleoptiles (seedling sheaths) of grass species like oats or corn. These coleoptiles exhibit strong positive phototropism.

  • This experiment helps establish the involvement of auxin in the phototropic responses of plants.

  • Further investigations can explore the effects of different auxin concentrations or inhibitors on phototropic bending.

Materials
  • Oat or corn seedlings with coleoptiles

  • Opaque covers with unilateral slits to control light direction

  • Agar blocks (growth medium)

  • Ruler

  • Scalpel

  • Petri dishes

  • Timer

Procedure
  1. Germinate seeds - Grow oats or corn seeds in moist paper towels or Petri dishes until coleoptiles reach a desired length (ex., 2-3 cm).

  2. Prepare experimental groups - Divide the seedlings into several groups:

    • Control group: Exposed to uniform light from all sides.

    • Unilateral light group: Exposed to light only from one side using the covers with slits.

    • Auxin-treated group (Optional): Apply a small amount of an auxin solution (ex. indole-3-acetic acid) to the cut tip (apex) of coleoptiles in the unilateral light group before light exposure.

  3. Record initial length - Measure and record the initial length of each coleoptile.

  4. Light exposure - Expose the seedlings in their respective groups to light for a specific duration (ex. 2-4 hours).

  5. Measure final length - After light exposure, measure the final length of each coleoptile.

  6. Safety Considerations: Use gloves and proper disposal techniques when handling plant hormones.

Analysis
  • Compare the average length changes in each group.

  • Unilateral light exposure is expected to cause bending towards the light source in the control and auxin-treated groups (if used).

  • The auxin-treated group may show a more pronounced bending response compared to the control, suggesting auxin's role in stimulating cell elongation in the shaded region.

Effects of Plant Hormones

Influence of gibberellins and ethylene (ethene) various plant functions.

Gibberellins (GA): involved in breaking seed dormancy (germination) in response to water, and bolting (production of flowers in an attempt to reproduce before death) in response to cold or lack of water.

  • A large family with over 130 identified types. The following are its key effects:

    • Stem elongation: Promoted by stimulating cell division and elongation in internodes (the stem regions between nodes).

      • This can be seen in the "bolting" phenomenon of some vegetables, where rapid stem growth occurs before flowering.

    • Seed germination: GA can break seed dormancy, allowing seeds to germinate under favorable conditions.

    • Fruit development: GA can stimulate fruit growth and development in some plants.

    • Parthenocarpy: In some cases, GA can induce fruit development without fertilization (parthenocarpy), a technique used in commercial agriculture for seedless fruits.

Ethylene (C2H4): Involved in the ripening of fruit and dropping of leaves.

  • A gas molecule that is a potent plant hormone with diverse effects such as:

    • Fruit ripening: Ethylene triggers the ripening process in fruits, causing changes in color, texture, and flavor.

    • Senescence: Ethylene promotes leaf senescence (ageing) and abscission (leaf drop), allowing plants to conserve resources and minimize water loss.

    • Epinasty: Ethylene can induce epinasty, a downward bending of stems and petioles (leaf stalks) in response to stress or injury.

    • Seed germination: Ethylene can inhibit seed germination in some plants while promoting it in others.

Use Plant Hormones to Control Plant Growth

Humans can exploit these responses and others such as triggering rooting in cuttings, by using plant hormones to trigger responses that are advantageous to us.

  1. Promoting Germination and Growth

    • Gibberellins can break seed dormancy and stimulate stem elongation which is beneficial for:

      • Overcoming seed dormancy: Certain fruits and vegetables have naturally dormant seeds that require specific conditions to germinate.

        • GA application can trigger germination, leading to more uniform and predictable crop establishment.

      • Elongation in dwarf varieties: GA can be used to promote stem elongation in dwarf crop varieties, allowing for better light interception and potentially higher yields.

  2. Enhancing Fruit Development and Ripening

    • Gibberellins (in some fruits) can enhance fruit size and development.

    • Ethylene is widely used in commercial agriculture to control fruit ripening which it's employed through:

      • Uniform Ripening: Ethylene gas treatment can be used to induce uniform ripening in fruits harvested before they reach full maturity.

        • Allows for better control over the harvest and transport process.

      • Off-Season Ripening: Certain fruits can be treated with ethylene to initiate ripening even when harvested outside their natural season.

  3. Weed Control

    • Auxin herbicides: Synthetic auxin-like molecules act as herbicides by disrupting plant growth and development.

      • These can target broadleaf weeds while leaving desired crops unharmed.

  4. Preventing Premature Fruit Drop

    • Auxins - Application can help prevent premature fruit drop in some fruit trees, reducing fruit loss and improves overall yield.

  5. Delaying Leaf Senescence and Abscission

    • Cytokinins: Hormones that can delay leaf senescence (aging) and abscission (leaf drop) which can be beneficial for:

      • Maintaining the appearance of ornamental plants - Cytokinin application can keep leaves green and healthy for a longer period, enhancing the aesthetic value of ornamental plants.

      • Extending the shelf life of harvested greens - Treatment with cytokinins can help maintain the freshness and quality of leafy greens like lettuce and spinach during storage and transportation.

Important Considerations
  • Specificity: Choosing the right hormone for the desired effect is crucial.

    • Using the wrong hormone can have unintended consequences.

  • Application method: Plant hormones can be applied in various ways, including foliar sprays, soil drenches, or seed treatments.

    • The appropriate method depends on the specific hormone and desired outcome.

  • Environmental regulations: The use of some plant hormones may be subject to regulations depending on the region.

B4.5 Should we use stem cells to treat damage and disease?

Stem cells offer the potential to treat patients by replacing damaged tissues or cells. However, the benefits must be weighed against risks and ethical concerns about the use and destruction of human embryos to collect embryonic stem cells. For these reasons, the use of stem cells in research and medicine is subject to government regulation in many countries.

Benefits of Stem Cell Therapy
  • Treatment of Incurable Diseases: Stem cells have the potential to treat currently incurable diseases like Parkinson's disease, Alzheimer's disease, and spinal cord injuries.

    • By replacing damaged cells or stimulating regeneration, stem cell therapies could offer a revolutionary approach to treatment.

  • Improved Organ Transplants: The limited availability of donor organs is a major hurdle in transplantation.

    • Stem cells could be used to grow new organs or tissues, eliminating the need for organ waiting lists.

  • Drug Discovery and Testing: Stem cells can be used to create cell models of diseases, allowing for more efficient and accurate drug discovery and testing.

Risks
  • Tumor Formation: A risk that transplanted stem cells could develop into tumors if they are not properly controlled.

  • Immune Rejection: In some cases, the body's immune system may reject transplanted stem cells, especially if they are derived from another person (allogeneic stem cells).

  • Ethical Concerns: The use of embryonic stem cells raises ethical concerns about the destruction of embryos.

Ethical Issues
  • Embryonic Stem Cells: The use of embryonic stem cells raises ethical questions about the moral status of an embryo.

  • Informed Consent: Obtaining informed consent from patients undergoing stem cell therapy can be challenging, especially if the long-term risks are not fully understood.

  • Fairness and Access: Stem cell therapies could become expensive, raising concerns about equitable access for all patients.