Genetics and Evolution Notes
Alleles
- Alleles are different forms of a gene.
- Example: Gene for eye color, alleles for brown, blue, green, or gray eyes.
Dominant vs. Recessive Alleles
- Dominant allele: Only one copy needed for expression.
- Recessive allele: Two copies needed for expression.
Genotype and Phenotype
- Genotype: Combination of alleles at a locus (e.g., big A little a).
- Homozygous: Two of the same alleles.
- Heterozygous: Two different alleles.
- Phenotype: Observable manifestation of a genotype (e.g., green eyes from big A little a genotype).
Dominance Types
- Complete dominance: Only one dominant allele.
- Codominance: More than one dominant allele is expressed.
- Incomplete dominance: No dominant allele; expression can be mixed or partial.
Penetrance
- Proportion of individuals with a specific allele who express the associated phenotype.
- Example: Some people with an allele for a condition may not show the phenotype.
Expressivity
- Variable phenotypic outcome of a genotype.
- Example: Genotype for extra fingers can result in different numbers of fingers (e.g., six or nine).
Genetic Leakage
- Flow of genes between species via hybrid offspring.
Genetic Drift
- Changes in the composition of a gene pool due to chance.
- Example: In a small population, one allele can dominate over time.
Founder Effect
- When a small group establishes a new population, their genes become disproportionately common.
- Leads to inbreeding.
Taxonomic Rank
- Mnemonic: King Philip Came Over From Great Spain (Kingdom, Phylum, Class, Order, Family, Genus, Species)
Mendel's Laws
- Law of Segregation: Two alleles for a gene separate during anaphase I of meiosis.
- Law of Independent Assortment: Alleles of different genes assort independently of one another (not true for linked genes).
- Linked genes: Genes located close together on the same chromosome are more likely to be inherited together.
Experiments Showing DNA as Genetic Material
- Griffith's experiment: Demonstrated transformation; bacteria could pick up DNA from their environment.
- Avery-MacLeod-McCarty experiment: Showed that degradation of DNA led to cessation of bacterial transformation while degradation of proteins did not, indicating DNA is the transforming factor.
- Hershey-Chase experiment: Radio-labeled DNA was found in bacteriophage-infected bacteria, confirming DNA as genetic material.
Types of Evolution
- Divergent, parallel, and convergent evolution.
Nucleotide Mutations
- Point mutation.
- Frameshift mutation.
- Results:
- Silent: No effect on protein.
- Missense: One amino acid replaced by another.
- Nonsense: Results in a stop codon, truncating the protein.
Frameshift Mutations
- Insertion or deletion of nucleotides, altering the reading frame of mRNA.
- Example: Adding 'A' to 'ATC' shifts the reading frame: 'AAT C'.
Results in Protein
- Silent: No effect on protein.
- Missense or Nonsense.
- Insertion or Deletion: Insertion of an amino acid into the amino acid chain.
Chromosomal Mutations
- Affect large segments of DNA on a chromosome.
- Types: Deletion, duplication, inversion, insertion, translocation.
Analytical Techniques
- Hardy-Weinberg Principle:
- p + q = 1 (for two alleles)
- p and q represent the frequencies of the two alleles.
- Assumes stagnant population with no evolution.
Recombination Frequency
- Likelihood of two alleles being separated during crossing over in meiosis.
- Crossing over can swap alleles on the same chromosome.
- Recombination frequency is related to the distance between genes; closer genes have lower recombination frequency.
Evolution
- Natural selection: Primary mechanism for evolution.
Modern Synthesis Model
- Mutation and recombination are mechanisms for variation.
Inclusive Fitness
- Allele frequencies remain constant in the absence of environmental change and evolution.
Punctuated Equilibrium
- Long periods of slow evolution followed by bursts of evolutionary activity.
Types of Natural Selection
Stabilizing, directional, disruptive, and adaptive.
Molecular Clock Model
- The degree of genomic difference between two species is related to the time since they diverged from a common ancestor.
- Species with a more recent common ancestor have more similar genomes.