Chapter 18 AP BIO
What are genes controlled by
Operons
2. What is a operator
The “switch” that controls dna transcription
3. What is the operator
A segment of dna locator in the promoter
4. How many genes can a operator control
1- several related genes
5. What is a operon
Operator + promoter + genes
6. What is a repressor
The “switch” protein
What binds to the operator
7. What happens if the repressor is attached to the operator
Operon is repressed or “turned off”
8. What happens if the repressor is not attached to the operator
Operon is not repressed or “ turned on” allowing for the gene to be transcribed
9. Review figure 18.3
10. Where is regulatory genes located
Away from operon
11. What do regulatory genes do
Code for repressor/ “switch” proteins
Continuously make the proteins
12. What do repressible operons do
Operon are turned on by default
No reppressor attached to operator
RNA polymerase can attach and transcribe
To turn off a repressor protein attaches to operator
13. What happens when a repressor protein is turned off
Blocks RNA polymerase attachment
It is reversible
Allosteric protein
Active and inactive shapes
Inactive state by default
Corepressors needed to activate
14. What do the allosteric proteins do
When activated, corepressor inhibits transcription of a specific gene.
15. What are the qualities of inducible operons
Operons are turned off by default
Reperessors attach to operator to turn on an inducer bonds inactivating it
Allosteric protein are active at default
16. What is an example of an inducible operon
Lac operon
17. What needs to happen when glucose is lacking but lactose is present
Bacteria need to change energy source
Must have enzymes to break down lactose
18. What does increases when glucose is lacking
cAMP [ ] increases
19. What does cAMP do
Activates CAP
20. What is CAP
An activator
21. What does CAP do
Binds to promoter of lac operon
Increases affinity of RNA polymerase for promoter of lac operon
Increase rate of transcription
22. What kind of control is lac operon under
Dual control
23. What is the negative controller of lac operon
Lac repressor
24. What does lac repressor do
Determines whether lac operon is transcribed at all
25. What is positive control of lac operon by
CAP
26. What does CAP do in lac operon
Determine the rate of transcription
27. What state is dna found in in non dividing cells
Chromatin
28. What are the two types of chromatin
Heterochromatin
Euchromatin
29. What is heterochromatin
Very tightly compacted chromatin
30. What is euchromatin
Less tightly compacted chromatin
31. Why does histone modification occur
Dut to the histones bonding with neighboring nucleosomes
32. What happens in histone modification
Chromatin becomes tightly compacted
33. What happens in histone acetylation
Acetyl groups (-COCH3) attaches to histones
Heterochromatin loosens into euchromatin
DNA transcription can occur
34. What happens in DNA methylation
Certain DNA bases become methylated
35. What does DNA methylation do
Prevents transcription
36. What could DNA methylation be a cause of
Cell differentiation
37. What can DNA methylation be passed on through
Cell division