Biochemical Tests for Unknowns 22-23

General Study Guide for Unknown Biochemical Tests

• Decarboxylase Tests

  • Ornithine (OD)

  • Lysine (LD)

  • Purpose: Detects decarboxylation of amino acids.

• Agar Types

  • Triple Sugar Iron Agar (TSI)

  • Urea Broth (UB)

  • Purpose: Differentiate enteric bacteria based on carbohydrate fermentation and hydrogen sulfide production.

• Broth Tests

  • Tryptone Broth (TRP): Indole Test

  • Methyl Red-Vogues Proskauer Broth (VP)

  • Methyl Red-Vogues Proskauer Broth (MR): Fermentation and mixed acid production.

• Nitrate Broth (NIT)

  • Purpose: Tests for nitrate reduction.

• Simmons Citrate (CIT)

  • Purpose: Tests for the ability to utilize citrate as a carbon source.

• Catalase Test (CAT)

  • Purpose: Tests for the enzyme catalase.

• Nutrient Gelatin (NG)

  • Purpose: Determines gelatin liquefaction.

• Sugar Fermentations

  • CH2O Fermentation

  • GLU (Glucose)

  • LAC (Lactose)

  • MAN (Mannitol)

  • MAL (Maltose)

  • SUC (Sucrose)

  • Purpose: Tests for sugar fermentation capabilities.

• Fluid Thioglycollate (FTM)

  • Purpose: Determines oxygen requirements.

• Motility Test Medium (MTM)

  • Purpose: Tests for bacterial motility.