Benchmark Quizzes
Module 1
Select all of the statements that are true about STRs:
First discovered by Kary Mullis
STR is the acronym for Short Trunkated Repeats
Multiple copies of an identical (or similar) DNA sequence arranged in direct succession where repeat sequence unit is 2 to 6 bp in length
AKA minisatellites
RFLP testing is more discriminatory that STR testing.
True
False
Peak height is measured in relative fluorescent units (RFU)
Module 2 Quiz 1
RNA is single stranded and DNA is double stranded.
In RNA In uracil pairs with Adenine.
DNA is stored in the nucleus and the mitochondria.
RNA transports proteins
Module 2 Quiz 2
During the s-phase, DNA and packaging proteins are duplicated, this takes approximately 10-12 hrs.
True
False
DNA from specific genes are transcribed into RNA than RNA is translated into an amino acid sequence, used to build proteins.
Helicase unwinds parental double helix at replication fork
DNA ligase joins Okazaki fragments of lagging strand
Topoisomerase breaks swivels and rejoins DNA
Endosymbiont theory states that… An early ancestor of eukaryotic cells engulfed a non-photosynthetic prokaryotic cell that used oxygen and it then developed into the mitochondria.
During interphase chromosomes are segregated and the cell divides.
true
false
Primase makes RNA primer at 5’ and the end of the lagging strand and at the 5’ end of each Okazaki fragment of the lagging strand
DNA polymerase III adds nucleotides to an RNA primer or pre-existing DNA strand
DNA polymerase I removes RNA primer from 5’end
Select all of the true statements:
In the nucleus, DNA is coiled around enzymes called histones to form chromatin
Each chromosome consists of multiple strands of DNA
Most human cells have 46 pairs of chromosomes
Chromatin condenses to from chromosomes
Module 3
Which of the following is an example of a situation where you would use the sum rule to determine probability?
The probability of picking a red card that is also a king from a standard deck of cards.
The probability of choosing 2 students from a class to take a test and both of them being boys.
The probability of picking 2 cards, one red and one black.
The probability of selecting one M&M candy from a bag and it being red or green.
The Law of Segregation states that two alleles for each trait separate (segregate) during gamete formation, and then unite at random, one from each parent, at fertilization.
True
False
Quiz 2
Base in one position of DNA molecule is replaced by one of the other bases. Substitution
Proportion of individuals in a population that express a particular phenotype. phenotype frequency
A mutation that changes a wild type allele of a gene to a different allele.
forward mutation
Group of interbreeding individuals of same species, inhabit same space at same time
population
Changes in the frequency of alleles within a population.
microevolution, genetic drift
Parts of two nonhomologous chromosomes change places.
Reciprocal Translocation
3/5 List the 5 assumptions for Hardy Weinberg Equilibrium
no migration
no natural selection
large population
random mating
no new mutations
Module 4 Quizzes
How many nanograms is equal to 49.4 grams?
49.4g x 1/10^-9ng = 4.94e+10 ng
How many milliliters are in a 2 L bottle of soda?
2000 ml
How many grams is equal to 80.4 mg?
.0804 g
How much salt is needed to make 0.58 L of a 2 mM salt-water solution (molecular weight of NaCL = 58.43 g/mol)?
0.068g
How many grams of acetic acid do you need to make 500.0 mL of 0.825 M acetic acid (molecular weight = 60.06 g/mol)?
24.8g
How much cherry juice (3.56 mM) is needed to make 7.54 mL of Shirley Temple (6.12 uM)?
12.96 uL
Module 5 Quizzes
Reduction is when a molecule gains an electron
The high dose hook effect causes false positive reactions in immunochromatographic tests.
false
Oxidation is when a molecule loses and electron
Reduction is when a molecule gains an electron
Select all of the following that are true about affinity:
overall strength of antibody-antigen complex
affinity is the strength of interaction btwn antigen and antibody at a single antigenic site
the lower the avidity the lower possibility of cross-reactivity
affinity is the strength of interaction btwn antibody and antigen at a single antigenic site
sum of attraction and repulsive forces btwn epitope and paratope
the lower the affinity the higher possibility of cross-reactivity
sum of the binding affinities of all the binding sites
Module 6 Quizzes
Select 3 examples of false positives for presumptive blood reactions
bleach
horseradish
metal salts
Which of the following describes chemiluminescence?
light is emitted at a lower wavelength than that of the excitation source
light emitted as a result of a chemical rxn
light is emitted at a higher wavelength than that of the excitation source
a fluorescent glow as a result of a chemical rxn
During a redox rxn, the compound that loses an electron is reduced
false
Christmas tree staining is considered by some to be the only true confirmatory test for human semen
false
Oligospermia is a medical condition that causes males to have lower sperm counts
Semen fluoresces under light that is 450-495 nm and viewed with an
orange goggle colored filter
Drag the words into the correct boxes
HSA is only found in human saliva false
amylopectin consists of long linear chains of glucose residues false
luminol is a chemiluminescent rxn to detect semen false
reduction is the loss of electrons false
Module 7 Quizzes
Module 8 Quizzes
Module 9 Quizzes
Module 10 Quizzes
Module 11 Quizzes
Module 12 Quizzes
QAS stands for Quality Assurance Standards
Accreditation is the formal recognition that an analyst meets or exceeds a list of standards
A developmental validation is commonly performed by an individual laboratory when beginning to run a new product
false
A performance check is required to be performed
following a repair
annually
following routine maintenance
Original observations data, and calculations shall be recorded immediately and shall be identifiable with the specific task.
Contains no intentionally added template DNA and used to monitor contamination from extraction to DNA typing. Reagent blanks
Contains no intentionally added template DNA and used to detect contamination contamination from reagents Negative control
Proficiency testing is a quality assurance measure used to monitor performance and identify areas in which improvement may be needed True
Module 13 Quizzes
The most common challenges to well settled forensic evidence in court involve: the integrity of the testing and the expert’s file
chain of custody involves documentation regarding the whereabouts of evidence during the testing process
The Frye test leaves the question of what scientific methodology is generally reliable to: the scientific community
The 6th Amendments protects: the right to confront witnesses against you
Expert witnesses are permitted to testify regarding an opinion they hold
true
Hearsay is an out of court statement offered for the truth of the matter asserted
The 6th Amendment applies to testimonial hearsay
Lab reports completed by forensic scientists for use in court are testimonial hearsay
true
A witness is qualified to testify as an expert if his or her specialized knowledge will assist:
the finer of fact (judge or jury)
Module 14 Quizzes

product identifier
signal word
hazard statements
precautionary statements
supplier identification
pictogram
Which of the following statements are true regarding handling chemicals
check container for expiration date
transport large bottles using leak-proof secondary containers
use the smallest volume possible
wear PPE
General safe practices are important for the health and safety of laboratory staff. Which of the following are general guidelines for maintaining a safe environment for laboratory activities?
know the location of safety equipment
always wear PPE
know what to do if an accident occurs
What are some of the safeguards necessary for safe laboratory operation
protective clothing
safety showers
sharps container
waste disposal
Keep all acids and bases in the same cabinet
false
What does RAMP stand for
recognize
asses
minimize
prepare
Always add water to acid, not acid to water
false