Final Exam

Brightfield Microscopy:

Why is a microscope used:

• To magnify, things that we cannot see at their actual size

Objective Lens:

• 10x, 20x, 40x, 100x

• Used to magnify objects on the stage

Stage:

• Holds slide

Condenser:

• Lightbulb below the stage

Fine Adjustment Knob:

• Provides dune tuning of focus

Coarse Adjustment Knob:

• Allows stage to be raised/lowered for quick focus

Calculating magnification power:

Eyepiece is 10x * objective lens amount

Example- 10x (eyepiece) x 40x (objective lens) = 400x total

Using 100x-

• When using 100x you need to use immersion out to lessen refraction of light