4.2 Eukaryotic

Eukaryotic Cells

Objectives

  • Understand the comparison of animal and plant cells.

  • Describe the structure of eukaryotic cells.

  • State the role of the plasma membrane.

  • Summarize the functions of the major cell organelles.

Eukaryotic Cells Overview

  • The phrase "form fits function" means an object's design should serve its purpose.

    • Example: In architecture, buildings are constructed to facilitate their intended activities.

Structure of Eukaryotic Cells

  • Eukaryotic cells have the following characteristics:

    • Membrane-bound nucleus (true nucleus).

    • Numerous membrane-bound organelles, including:

    • Endoplasmic reticulum.

    • Mitochondria.

    • Specialized compartments with specific functions, akin to organs in the human body.

    • Examples:

      • Endoplasmic reticulum.

      • Golgi apparatus.

      • Chloroplasts.

      • Mitochondria.

    • Several chromosomes, which are structures made of DNA, housed within the nucleus.

Plasma Membrane

  • Eukaryotic cells possess a plasma membrane similar to prokaryotes.

  • It is defined as:

    • A phospholipid bilayer embedded with proteins.

    • Function: To separate the cell from its environment.

  • Main functions include:

    • Regulating the movement of organic molecules, ions, water, and oxygen (O) into and out of the cell.

    • Allowing waste products such as carbon dioxide (CO2) and ammonia (NH3) to exit the cell.

Cytoplasm

  • The cytoplasm is defined as the region between the plasma membrane and nuclear envelope.

    • Contains organelles suspended in gel-like cytosol (the aqueous portion of the cytoplasm).

    • Composition:

    • 70-80% water, but exhibiting a semisolid consistency due to present proteins.

    • Contains small molecules such as glucose, other sugars, amino acids, nucleic acids, fatty acids, and glycerol derivatives.

    • Includes dissolved ions like sodium (Na+), potassium (K+), and calcium (Ca2+).

    • Functions: Hosts many metabolic reactions, including protein synthesis.

Nucleus

Structure and Function
  • The nucleus houses the cell's DNA and directs the synthesis of ribosomes and proteins.

  • Structure:

    • The nuclear envelope is a double-membrane structure that forms the nucleus’s outermost layer.

    • Nuclear pores regulate the movement of ions, molecules, and RNA between the nucleoplasm (the semisolid fluid that contains chromatin and nucleolus) and the cytoplasm.

Chromosome Structure
  • Contains chromosomes, which are linear DNA-protein structures, having a specific number per eukaryotic species.

  • Chromatin exists in:

    • Condensed form (during cell division).

    • Decondensed form (non-dividing).

  • The nucleolus is a specific area within the nucleus responsible for the assembly and export of ribosomal subunits to the cytoplasm, utilizing sections of DNA that encode for ribosomal RNA.

Ribosomes

Overview
  • Ribosomes are cellular organelles vital for protein synthesis.

  • Locations: Ribosomes can be attached to:

    • Plasma membrane.

    • Endoplasmic reticulum.

    • Nuclear envelope membranes.

  • Their location correlates with the intended destination of the proteins synthesized.

Structure
  • Ribosomes are large protein-RNA complexes consisting of:

    • Two subunits.

  • The mRNA from the nucleus provides the code that ribosomes translate into specific protein sequences.

  • Cells that synthesize high quantities of proteins, such as those producing pancreatic enzymes, contain a high number of ribosomes.

Mitochondria

  • Mitochondria function as the cell's powerhouses, producing ATP, which is the primary energy currency, through the process of cellular respiration that utilizes chemical energy found in molecules like glucose.

  • Structure:

    • Composed of double membranes with inner folds (named cristae), a matrix, and their own ribosomes and DNA for specialized energy production.

  • Example of function: Muscle cells are rich in mitochondria due to their high energy demands; in cases of oxygen deficiency, lactic acid is produced instead of ATP.

Peroxisomes

  • Peroxisomes are described as single-membrane organelles that execute oxidation reactions and detoxification processes.

  • They serve to:

    • Break down fatty acids, amino acids, and hydrogen peroxide (H2O2) through oxidation.

    • Detoxify alcohol in liver cells.

    • Participate in metabolism, defense, and response to stress in plants.

    • Specialized forms called glyoxysomes facilitate the conversion of fats to sugars.

Vesicles and Vacuoles

  • Vesicles and vacuoles are membrane-bound sacs utilized for storage and transport.

  • Key differences:

    • Vacuoles are typically larger than vesicles.

    • Vesicles can fuse with other membranes to release contents, while vacuoles remain separate.

  • In plants, vacuoles contain enzymes that degrade macromolecules.

Comparison of Animal Cells and Plant Cells

  • Differences in Structure:

    • Animal cells have centrosomes and lysosomes, which are absent in plant cells.

    • Plant cells contain a cell wall, chloroplasts, additional plastids, and a large central vacuole, all of which are not found in animal cells.

  • Both types of cells share common structures such as the plasma membrane, cytoplasm, nucleus, ribosomes, mitochondria, peroxisomes, and microtubule-organizing centers (MTOCs).

Centrosome

  • The centrosome acts as a microtubule-organizing center located near the nuclei of animal cells.

  • Function:

    • Contains centrioles organized in microtubule triplets, crucial for organizing microtubules and assisting in the separation of chromosomes during cell division.

  • However, centrosomes are non-essential for cell division since cells devoid of them, as well as plant cells, can still divide.

Lysosomes

  • Lysosomes function as the cell's