Bio111 Exam 2- Module 5- TAMU- Fletcher

🧠 CELL MEMBRANES & TRANSPORT – STUDY NOTES

Part 1: Cell Membrane Structure & Fluid Mosaic Model

Fluid Mosaic Model:

  • Describes the cell membrane as a fluid (flexible) layer made of phospholipids, with a mosaic of proteins, cholesterol, and carbohydrates embedded within.

  • Fluid → components move sideways within the layer.

  • Mosaic → diverse molecules like proteins and lipids make up the structure.

Membrane Components:

  • Phospholipids: Form the bilayer; hydrophilic (water-loving) heads face outward, hydrophobic (water-fearing) tails face inward.

  • Cholesterol: Scattered within phospholipids; stabilizes membrane and affects fluidity.

  • Integral proteins: Span the membrane; involved in transport and signaling.

  • Peripheral proteins: On surface; support and communication roles.

Asymmetry:

  • The inner and outer layers differ in composition and function.

  • Glycolipids/glycoproteins are mainly on the outer layer; different proteins face different directions.

  • Maintains selective interactions and signaling.

Factors Affecting Fluidity:

  1. Saturated vs. Unsaturated fats:

    • Saturated = less fluid (straight tails).

    • Unsaturated = more fluid (kinks prevent tight packing).

  2. Cholesterol:

    • Adds stability; prevents too much movement or freezing.

  3. Temperature:

    • Higher temp → more fluid; lower temp → less fluid.

Part 2: Movement Across Membranes

Key Terms:

  • Diffusion: Movement of molecules from high → low concentration.

  • Osmosis: Diffusion of water through a selectively permeable membrane.

  • Amphipathic: Molecule with both hydrophobic and hydrophilic parts (ex: phospholipids).

  • Electrogenic: Generates an electrical charge difference (like ion pumps).

Molecules That Pass Easily:

  • Small, nonpolar molecules: O₂, CO₂, N₂ → diffuse easily.

  • Small polar molecules (H₂O) → slower or through channels.

  • Large or charged molecules → need transport proteins.

Tonicity Effects on Animal Cells:

  • Hypotonic solution: Water enters cell → cell swells or bursts.

  • Isotonic solution: Water moves equally → cell stays the same.

  • Hypertonic solution: Water leaves cell → cell shrinks.

Simple vs. Facilitated Diffusion:

  • Simple: Molecules pass directly through bilayer (no proteins, no energy).

  • Facilitated: Uses transport proteins (channel or carrier) to move molecules down their gradient.

Part 3: Passive vs. Active Transport

Passive Transport:

  • No energy needed; moves down concentration gradient (high → low).

  • Includes: diffusion, osmosis, facilitated diffusion.

Active Transport:

  • Requires energy (ATP); moves substances against gradient (low → high).

  • Needs carrier proteins (pumps).

Conditions for Active Transport:

  • Requires ATP and a specific membrane protein.

  • Often used to maintain ion gradients.

Sodium-Potassium Pump:

  • Moves 3 Na⁺ out, 2 K⁺ in using ATP.

  • Maintains charge difference (negative inside).

  • Example of electrogenic pump → creates electrochemical gradient.

Electrochemical Gradient:

  • Combination of ion concentration difference and electrical charge across membrane.

  • Cells are more negative inside.

  • Provides stored energy for cellular processes.

Co-Transport (Secondary Active Transport):

  • Uses energy stored in one gradient (like H⁺ or Na⁺) to move another molecule against its gradient.

  • Example: glucose absorption in intestines with Na⁺ ions.

Part 4: Bulk Transport

Bulk Transport:

  • Moves large molecules or materials using vesicles.

  • Requires energy → active transport.

Endocytosis Types:

  1. Phagocytosis: “Cell eating” – engulfing solids or large particles (ex: white blood cells eating bacteria).

  2. Pinocytosis: “Cell drinking” – taking in fluids and dissolved substances.

  3. Receptor-Mediated Endocytosis: Specific molecules bind to receptors and get internalized (ex: LDL cholesterol uptake).

Endocytosis vs. Exocytosis:

  • Endocytosis: Imports material into the cell (enter).

  • Exocytosis: Exports material out of the cell (exit).

  • Both use vesicles and energy.