Exercise Physiology: ATP Systems, Metabolic & Cardiovascular Responses
ATP Production Pathways in Skeletal Muscle
Three concurrent, layered systems supply to working muscle fibres
Aerobic (cellular) respiration – mitochondria use oxygen, glucose & triglycerides. • Dominant while oxygen delivery is sufficient.
Anaerobic respiration (lactic-acid fermentation) – cytosolic glycolysis continues when aerobic flux is inadequate
Substrates = glucose, glycerol component of triglycerides.
Produces lactate, ↓pH
Phosphocreatine (PCr) shuttle – single-step, enzyme-catalysed phosphate transfer.
Creatine + ATP $\xrightarrow{\text{creatine kinase}} $ PCr + ADP (occurs at rest).
When [ATP] drops: PCr + ADP → ATP + creatine (one fast reaction).
Acts as an “external battery” that re-charges during rest (minutes).
Limited by total creatine & ATP pools.
Creatine Supplementation
• Oral creatine monohydrate ↑ intramuscular creatine → ↑ PCr “battery” size.
• Functional outcomes (typical):
• Greater ability to resynthesise ATP during brief, high-power efforts.
• ≈ 5 % improvement in force/volume; small but meaningful in competitive sport.
Hemoglobin O2 Dissociation During Exercise
Hemoglobin curve at rest
Aveolar O2 pressure ~ 100 mmHg → Hemoglobin saturation = 100%
Resting tissue O2 pressure = 40 mmHg
Hemoglobin curve during exercise:
Saturation is around 75 mmHg
Resting tissue saturation drops to 23%
contracting skeletal muscle O2 pressure can fall to 23 mmHg
Hb saturation ≈ ⇒ additional 35 % unloaded DURING EXCERCISE
• Net O2 release ≈ 60 % of Hb content during intense exercise.
Metabolic Consequences of Increasing Intensity
↑ motor-unit recruitment → ↑ cross-bridge cycling → ↑ ATP demand.
Aerobic output ramps until limited (often by O2 delivery).
Beyond limit: anaerobic ATP production ↑; lactic acid increases, increases H+ ↓ pH.
By-products: ↑ CO2, heat, catecholamines.
Feed-Forward vs Feedback Control at Exercise Onset
• Observed: ventilation & cardiac output jump within seconds – before acidosis, ↑PCO2 or ↓BP develop.
• Mechanism: “Central Command” feed-forward signal from higher brain centres (limbic, motor cortex).
• Resets set-points in medullary respiratory & cardiovascular centres.
• Feedback (chemo- & baro-receptors) then maintains new set-points.
• Anticipatory rise: HR often elevates on start line via same pathway.
Substrate Utilisation Across Intensities
At rest: ≈ 70 % ATP from fats, 30 % from carbohydrates.
With rising VO2 , there’s a decrease in fat usage, and in increase from carbohydrate usage
Fast twich fibers and epinephrine favor glycolysis
Total energy demand also increases, so absolute fat oxidation (blue fat % x total ATP rate) peaks near 60% VO2
VO(_2$$ Kinetics, Oxygen Deficit & EPOC
Exercise energy demand rises instantly, but measured VO2 lags 2-3 minutes which creates an oxygen deficit
Oyxgen deficit is govered by PCr breakdown, anaeorobic glycolysis, and myoglobin O2 storage
Post-exercise VO2 remains elevated (Excess Post-exercise O2 consumption- EPOC on the graph)
Rapid phase (≤3 min) – replenishes PCr, myoglobin O2 restores ion gradients.
• Slow phase (≤30 + min) – lactate → glucose (Cori cycle), thermogenic & catecholamine effects maintain high metabolic rate.
• Hyperventilating before work only modestly reduces deficit; cardiac output ramp remains limiting.
Cardiopulmonary Variables: Low vs High Intensity
Main comparisons (rest → low → high intensity):
Predominant fuel: fat → mixed → carbohydrate.
Main ATP pathway: aerobic → aerobic + anaerobic → anaerobic-dominant.
Motor units: small (Type I) → recruit larger (Type IIa) → all types
Ventilation: low → increases proportionally
Arterial Partial O2 pressure stays around 100 mmHg at ALL intensities
regulated by increased ventilation
Cardiac output & systolic BP progressively increase
Diastolic BP stays stable due to muscle vasodialation
Stroke volume increases and plateaus around 40% VO2 because reduced diastolic filling time offsets rising contractility
Left-Ventricular Pressure–Volume (PV) Loops in Exercise
• Intensities labelled Rest (C) → Light (A) → Heavy (B):
• End-systolic volume (ESV) ↓ as contractility ↑.
• Systolic pressure ↑ with intensity (reflects contractility).
• End-diastolic volume (EDV) ↑ from rest to moderate (more venous return) then ↓ at very high intensity (short diastole).
• Net result: loop becomes taller (higher pressure) & narrower (lower ESV); area (stroke work) ↑ until SV plateau.
Musculoskeletal Considerations
• Knee pain during leg extensions/squats usually due to:
• Overloading too quickly.
• Unresolved prior injury.
• Solutions: regress load, progress gradually, address pre-existing pathology, choose alternate movements.
Practical / Ethical / Application Notes
• Creatine is legal, inexpensive, generally safe
• Understanding feed-forward control aids clinical interpretation (e.g.
hyperventilation ≠ pathology if patient just climbed stairs).
• EPOC’s modest extra fat oxidation is not a major weight-loss tool by itself; intensity & duration still matter.