Exp 3

Understand how TLC separates compounds. • Label each part of a developed TLC plate. • Understand why a TLC developing chamber is set up the way it is. • Calculate Rf (know equation). • Rank mobile phase polarity. • Relate mobile phase polarity to Rf. • Relate compound polarity to Rf. • Be able to interpret TLC plates. • Know the effect of common issues in handling TLC plates (e.g., why pen should not be used, why the solvent front needs to be marked immediately).