Lab Exam Review - Protozoa

Amoeba sp.

  • Live in fresh and salt water, and in moist soil

  • Bottom dwellers

  • Require a surface on which to glide

  • Feed on bacteria, algae and other protozoa

Support:

  • Cell membrane — semi permeable to facilitate exchange of products

  • Ectoplasm — beneath cell membrane (made of fibrous actin)

  • Endoplasm — contains cytoskeleton and organelles

Movement:

  • Psuedopodia — grasps surface pulls rest of cell forward

    • Endoplasm in pseudopodia stiffens into ectoplasm, and existing ectoplasm softens into endoplasm to facilitate a moving stream forward

Feeding/digestion:

  • Phagocytosis

    • Encircle food items with pseudopodia

    • Form water filled food vacuoles that are later digested by enzymes

    • Waste products and excess water removed by semipermeable cell membrane

Reproduction

  • Asexual reproduction by binary fission

    • Organism copies DNA, organism splits into two, each new organism has nucleus with genetic material

Amoebazoa

Paramecium sp.

  • Ciliate protozoa

  • Found in freshwater containing much organic material

  • Feed on small particles (e.g. bacteria)

Support:

  • Cell membrane

  • Ectoplasm

  • Endoplasm — contains cytoskeleton and organelles

Movement:

  • Oar-stroke actions of cilia that move the organism forward or in circles

Feeding/digestion:

  • Cilia-lined oral groove draws food particles into endoplasm, food vacuoles form for later digestion

  • Waste products removed by semi-permeable cell membrane

  • Contractile vacuoles are responsible for osmotic balance — pumping out excess water

Reproduction:

  • Asexual — binary fission

  • Sexual — conjugation

Cilia, ectoplasm, endoplasm, oral groove, food vacuole, contractile vacuole

Trypanosoma sp.

  • Parasite that can cause serious illness

    • (e.g. sleeping sickness and Chaga’s disease)

  • Requires intermediate blood-sucking host (e.g. mosquito) to infect human hosts

Support:

  • Cell membrane

  • Ectoplasm

  • Endoplasm

Movement:

  • Whip-like organelle called flagellum that moves organism through fluid environment

Feeding/digestion:

  • Absorb food from host’s blood or other body fluids through cell membrane

Reproduction:

  • Sexual — within insect vector

  • Asexual — binary fission within human host