Cell Cycle Overview
The Cell Cycle
Cell Division
Essential for single-celled organisms and multicellular eukaryotes.
Roles include asexual reproduction, growth, development, and tissue renewal.
Cell Cycle Phases
Interphase: Growth, DNA replication, preparation for division.
Subdivided into G₁, S, and G₂ phases.
Mitosis: Division of genetic material.
Stages: prophase, prometaphase, metaphase, anaphase, telophase.
Cytokinesis: Division of cytoplasm, results in two daughter cells.
Chromosomal Structure
Eukaryotic DNA organized into chromosomes consisting of chromatin.
Gametes have half the chromosomes compared to somatic cells.
Sister chromatids connected at centromeres, separate during anaphase.
Mitotic Spindle
Composed of microtubules to control chromosome movement.
Centrioles replicate during interphase and migrate during cell division.
Cell Cycle Control System
Directed by signaling molecules with specific checkpoints.
Cyclins and cyclin-dependent kinases (Cdks) regulate progression through the cell cycle.
MPF (maturation-promoting factor) triggers the M phase.
Checkpoints
G₁, G₂, and M checkpoints assess cell condition and readiness for division.
Abnormal signals can lead to exit into G0 phase (non-dividing state).
External Influences on Cell Division
Growth factors stimulate cell division.
Density-dependent inhibition prevents overcrowding; anchorage dependence required to divide.
Cancer Cells
Exhibit uncontrolled growth; do not follow density-dependent or anchorage-dependent regulations.
Treated with chemotherapeutic drugs that affect the cell cycle, potentially targeting fast-dividing normal cells as well.