Assessment 3 Notes & Feedback Access

Log in → navigate to “Feedback Resources” section
Read the “How to access your feedback” document first
Click the special marks link (new Turnitin-free submission point created so that a rubric/PDF can be attached)
- Ignore the “Assessment overdue” warning – nothing needed from you there
Under “Feedback” you will find
- Your preliminary mark out of 46 (written document)
- Downloadable rubric (Word/PDF) containing detailed comments

Think rubric feedback is unfair? → lodge official remark request via provided link within 5 business days (deadline next Wed 5 pm)
Whole task is re-graded by a new assessor; mark can go up or down

Slides, template, data files and step-by-step Excel video are already on the Assessment Table
- Download slides to annotate during lecture
Components you will produce:
1. Diagnosis of Alice (healthy / pre-diabetic / diabetic)
2. Analysis of her food & activity journal + recommendations
3. Personal reflection
4. References (≥ 6, Vancouver style)

25 y, weight 45 kg, height 1.65 m
Symptoms: tired, lethargic, blurry vision, tingling
Fasting bloods
- Glucose 5.7 mmol L⁻¹ (normal $3.4–5.4$ ) – slightly high
- Total cholesterol 5.3 mmol L⁻¹ (upper-normal)
- Triglycerides 2.2 mmol L⁻¹ (normal ≤ 2.0) – elevated
- HDL 1.2 mmol L⁻¹ (> 1 desirable) – good
- LDL 4.0 mmol L⁻¹ (upper-normal)
GP orders:
- Oral Glucose Tolerance Test (GTT)
- 3-day weighed food diary + full activity log

Meal → CHO → digested to glucose → absorbed to blood → hyperglycaemia
Pancreas senses ↑glucose → secretes insulin
Insulin binds insulin receptor (IR) on target cells (e.g. skeletal muscle)
- Activates signalling cascade → translocates GLUT4 to membrane
- Glucose enters cell for:
- ATP production (glycolysis → TCA → OXPHOS)
- Glycogen synthesis

Type	Problem	% cases
1	Pancreas produces no insulin	≈ 5 %
2	Insulin produced but tissues insulin-resistant	≈ 95 %

Early marker for Type 2 risk = insulin resistance (↑insulin needed to handle same glucose load)

Protocol
- Overnight fast (8–12 h)
- Drink 75 g–150 g pure glucose at $t = 0$
- Blood samples every 30 min for 3 h (7 time points)
- Subject rests quietly (no food, stress, exercise)
Interpretation goal = degree & duration of hyperglycaemia compared with healthy range (blood glucose should fall to baseline within ≈ 2 h)

Direct spectrophotometry useless (glucose low UV absorbance)
Convert glucose → $\text{Glucose-6-P}$ via hexokinase + ATP
Convert $\text{G-6-P}$ → $\text{6-phosphogluconolactone}$ + NADPH via G-6-P dehydrogenase
NADPH strongly absorbs at $340\,\text{nm}$
- Absorbance $A_{340} \propto$ [glucose]

Reagents per well
- 50 µL sample (1:10 serum dilution)
- 200 µL “Glucose Reagent” (contains ATP, hexokinase, NADP⁺, G-6-PDH)
Standards: serial volumes of a 2 mmol L⁻¹ glucose standard (0–50 µL) + water to 50 µL total
Incubate 15 min → read $A_{340}$

Triplicate readings → mean absorbance
Blank-correct (subtract background)
Build standard curve (amount vs. absorbance) → linear $y = m x$
- $y =$ absorbance ; $x =$ µmol glucose ; $m =$ slope
For each unknown: $x = \dfrac{y}{m}$ → convert to concentration
- Adjust for 1:10 dilution: $[\text{undiluted}] = 10 \times [\text{diluted}]$
Plot Glucose vs. Time for Alice & healthy control, include reference lines for diagnostic cut-offs

Diagnosis (10 marks)
- Standard-curve graph + Glucose-vs-time graph (healthy vs Alice)
- Diagnostic thresholds (cite source). Conclude: Normal / Pre-DM / DM
- Comment on initial fasting lipid/glucose results
- Explain purpose of healthy control (experimental control validity)
General Health Stats (10 marks)
- Use online risk calculator (may return an error → interpret)
- Compute $BMI = \dfrac{weight}{height^2}$ ; comment
- Calculate daily energy intake (kcal/kJ) vs expenditure (METs) to see surplus/deficit
Diet Analysis (10 marks)
- Portion sizes, macronutrient % (CHO, Fat, Protein)
- Identify red flags (e.g. > recommended free sugars, low fibre)
- Evidence-based recommendations with scientific rationale & examples
Activity Analysis (6 marks)
- Discuss MET values (1 = rest) of listed activities
- Sedentary vs. moderate/vigorous minutes; align with guidelines (e.g. $\ge 150$ min moderate/wk)
- Recommendations & justification
Personal Reflection (10 marks)
- Your approximate intake/expenditure, BMI, fitness goals
- Compare & contrast with Alice (similarities / differences)
References (2 marks)
- ≥ 6 credible sources (journals, gov/WHO websites, lecture notes) in Vancouver (numbered) format

Use provided template; save as PDF (Word accepted but PDF preserves layout)
Include graphs as embedded images (Excel → copy-paste or screenshot)
Do not submit the two Excel workbooks; keep for records
AI policy
- Permitted only for grammar/format polishing
- Keep original drafts; may be requested

Start Excel analysis early; follow the 15-min instructional video
Always use Excel formulas (avoid manual rounding)
Unit conversions: $1\,\text{cal} = 4.184\,\text{kJ}$
Reference lectures after Week 5 (METs, nutrition guidelines, diabetes pathophysiology) heavily

Practice exam (Week 8 tutorial) will mimic real Inspira interface
After practice session tutor will reveal breakdown: number of MCQs, short answers, medium responses, etc.
Medium-response (~5 marks) expects concise yet evidence-rich paragraphs; include examples & brief rationale but no diagrams can be uploaded (exam system limitation)

Provide brief context before diving into comparison/analysis
Use bullet lists and explanatory sentences
Include definitions, similarities and differences when asked to “compare & contrast”
Always justify recommendations with mechanisms or data (e.g. "unsaturated fat lowers LDL via …")
Where helpful, reference numerical guidelines (e.g. < 10 % energy from saturated fat)

$BMI = \dfrac{kg}{m^2}$
OGTT normal fasting: $3.4 \text{–} 5.4\,\text{mmol L}^{-1}$
OGTT 2-h cut-offs (WHO):
- < 7.8\,\text{mmol L}^{-1} = Normal
- $7.8 \text{–} 11.0$ = Impaired Glucose Tolerance (Pre-DM)
- $\ge 11.1$ = Diabetes
Energy densities
- CHO & Protein: 4 kcal g⁻¹
- Fat: 9 kcal g⁻¹
Dilution correction: $C<em>{orig} = DF \times C</em>{diluted}$ ; here $DF = 10$