viruses

Viruses

Overview

  • Estimated 10 nonillion (10,000,000,000,000,000,000,000,000,000) individual viruses exist on our planet.

    • Enough to assign one to each star in the universe.

  • Debate on Existence of Life: Scientists discuss whether viruses can be classified as alive. Most scientists conclude that viruses are nonliving.

Importance of Viruses

  • Retroviral Genomes: The human genome contains approximately 500,000 retroviral genomes, which accounts for about 8% of the genome's total length.

    • These retroviral genomes are termed viral fossils, being genes inserted by retroviruses.

  • Syncytin: A protein involved in fusing placenta cells to form a wall, encoded by the envelope gene of a human endogenous retrovirus (HERV-W).

    • Its role in placental development is considered very significant.

  • Pandemics Caused by Viruses:

    • The Black Death: Approximately 25 million deaths.

    • Asian Flu: Around 1.1 million deaths.

    • AIDS: Estimated 35 million lives lost.

    • Spanish Flu: 50 million deaths.

    • COVID-19: Over 7 million deaths.

Structural Features of Viruses

  • Size: Extremely small and fixed size, ranging from 10 to 400 nm.

  • Genetic Material: Contain either RNA or DNA as their genetic material.

  • Capsid: Enclosed by a boundary made of a protein called capsid.

    • Does not contain cytoplasm inside, is hollow except for RNA/DNA.

  • Enzymes: Possess few, if any, enzymes.

  • Capsid Structure: The unique composition and structure of the capsid—based on specific amino acids—determines the virus's ability to infect particular hosts.

Bacteriophages

  • A bacteriophage is a virus that specifically infects bacteria.

    • Bacteriophage lambda: Infects E. Coli.

    • Features:

      • A capsid head that protects the double-stranded DNA core.

      • Tail fibers that attach the virus to the cell.

      • A tail sheath made of proteins that contracts to drive the tail tube through the host cell's outer membrane.

      • DNA is injected through the tail into the host cell.

Coronaviruses

  • The COVID-19 pandemic has led to over 5 million deaths globally, with more than 205 million cases.

  • Coronaviruses: Characterized by:

    • Spherical shape.

    • Single stranded RNA.

    • An envelope surrounding the capsid.

    • Numerous spike projections on the envelope.

HIV (Human Immunodeficiency Virus)

  • HIV is an STD transmitted through promiscuity.

    • Features:

    • Has an envelope outside the capsid.

    • Contains two identical strands of RNA protected by the capsid.

    • Encodes reverse transcriptase, allowing for DNA production from the viral RNA template.

  • Designated as a retrovirus due to its ability to make a DNA copy of its RNA code.

  • Envelope spikes of HIV are made of proteins and carbohydrates.

    • A mother with AIDS must undergo a C-Section to prevent the transmission of AIDS to the child during birth, as the placenta filters maternal blood, keeping the infant safe until natural delivery.

Host Specificity

  • Certain viruses specifically bond to certain host cells:

    • HIV: Attaches to white blood cells (CD4 cells).

    • Poliovirus: Targets spinal nerve cells.

    • Hepatitis Virus: Infects liver cells.

    • Norovirus: Infects intestinal system cells.

Life Cycle of Viruses

Lytic Cycle
  1. Attachment: Virus attaches to a specific site on the host cell.

  2. Penetration: Incorporates genetic material into the host cell's cytoplasm.

  3. Biosynthesis: Uses the host's processes to produce new viral components.

  4. Maturation: Assembles viral components into new cell entities.

  5. Release: Bursts the host cell and releases new viruses into the surrounding environment.

Lysogenic Cycle
  • Does not result in immediate release of the new virus.

  • The bacteriophage's DNA integrates with bacterial DNA, forming a prophage.

  • This prophage does not alter the bacterial DNA, allowing the bacteria to continue normal functions, including reproduction.

  • Subsequent bacterial generations will carry the prophage within their genome.

Origin of Viruses

  • Virus First Hypothesis: Suggests that viruses originated before cells due to their simplicity and may have contributed materials for developing first cells.

  • Regressive Hypothesis (Reduction or Degeneracy Theory): Proposes that viruses were once small cells that became parasites, shedding unnecessary structures through gene reduction.

  • Escape Hypothesis (Vagrancy Hypothesis): Indicates that portions of genetic material from larger organisms escaped and formed viruses after being surrounded by a protective boundary.

  • Each hypothesis faces challenges for credibility, and it’s possible that one, none, or all hypotheses could be correct.

  • Convergent Evolution: Suggests that viruses developed similar traits due to environmental conditions, indicating commonalities:

    • Obligate parasites; cannot replicate or perform life functions alone.

    • Possess a protein outer boundary (capsid) without cytoplasm.

    • Share genetic material (DNA or RNA) between viruses and all of Earth's organisms.

Vaccination and Viral Evolution

  • Annual vaccinations for influenza and HIV are necessary due to the rapid evolution of these viruses through mutation.

  • Antigenic Drift produces small incremental changes over long periods.

  • Antigenic Shift occurs when two or more viruses infect the same cell and combine genetic material, creating major changes rapidly.

    • Antigenic drift leads to slow variation in viral surface proteins.

    • Antigenic shift results in entirely new viruses that can potentially form pandemics as the immune system does not recognize them.

  • Notably, HIV can undergo rapid antigenic drift, complicating the immune response.

  • Influenza can undergo antigenic shift, resulting in new virus strains that are unrecognized by the immune system.

Vaccines
  • Vaccines are useful in preventing and controlling many viruses.

  • Influenza vaccines must be updated annually due to antigenic drift, which occurs at a manageable pace for vaccine adjustments.

  • Antigenic shifts present challenges for annual vaccines because the rapid changes make it unpredictable, significantly complicating successful vaccination efforts.