The Fundamental Unit Of Life

• In 1665 , Robert Hooke was examining a slice of cork (a substance which comes from the bark of a tree) through a self - discovered microscope , he noticed that cork had a honeycomb-like structure , with many small compartments. ‘cell ‘ is a latin word for ‘ little room ‘ .
• skin of an onion bulb = epidermis \n
• Cells were first discovered by Robert Hooke in 1665. He observed the cells in a cork slice with the help of a primitive microscope.
• Leeuwenhoek (1674), with the improved microscope, discovered the free living cells in pond water for the first time. It was Robert Brown in 1831 who discovered the nucleus in the cell.
• Purkinje in 1839 coined the term ‘protoplasm’ for the fluid substance of the cell.
• The cell theory, that all the plants and animals are composed of cells and that the cell is the basic unit of life, was presented by two biologists, Schleiden (1838) and Schwann (1839).
• The cell theory was further expanded by Virchow (1855) by suggesting that all cells arise from pre-existing cells. With the discovery of the electron microscope in 1940, it was possible to observe and understand the complex structure of the cell and its various organelles.
• It is now known that a single cell may constitute a whole organism as in Amoeba,Chlamydomonas, Paramoecium and bacteria. These organisms are called unicellular organisms (uni = single). On the other hand, many cells group together in a single body and assume different functions in it to form various body parts in multicellular organisms (multi = many) such as some fungi, plants and animals.

Unicellular freshwater organisms and most plant cells tend to gain water through osmosis. Absorption of water by plant roots is also an example of osmosis. Thus, diffusion is important in exhange of gases and water in the life of a cell. In additions to this, the cell also obtains nutrition from its environment. Different molecules move in and out of the cell through a type of transport requiring use of energy. The plasma membrane is flexible and is made up of organic molecules called lipids and proteins. However, we can observe the structure of the plasma membrane only through an electron microscope. The flexibility of the cell membrane also enables the cell to engulf in food and other material from its external environment. Such processes are known as endocytosis. Amoeba acquires its food through such processes.

• CELL WALL

Plant cells, in addition to the plasma membrane, have another rigid outer covering called the cell wall. The cell wall lies outside the plasma membrane. The plant cell wall is mainly composed of cellulose. Cellulose is a complex substance and provides structural strength to plants. When a living plant cell loses water through osmosis there is shrinkage or contraction of the contents of the cell away from the cell wall. This phenomenon is known as plasmolysis.

Cell walls permit the cells of plants, fungi and bacteria to withstand very dilute (hypotonic) external media without bursting. In such media the cells tend to take up water by osmosis. The cell swells, building up pressure against the cell wall. The wall exerts an equal pressure against the swollen cell. Because of their walls, such cells can withstand much greater changes in the surrounding medium than animal cells.

• NUCLEUS

The nucleus has a double layered covering called nuclear membrane. The nuclear membrane has pores which allow the transfer of material from inside the nucleus to its outside, that is, to the cytoplasmThe nucleus has a double layered covering called nuclear membrane. The nuclear membrane has pores which allow the transfer of material from inside the nucleus to its outside, that is, to the cytoplasm. The nucleus contains chromosomes, which are visible as rod-shaped structures only when the cell is about to divide. Chromosomes contain information for inheritance of characters from parents to next generation in the form of DNA (Deoxyribo Nucleic Acid) molecules. Chromosomes are composed of DNA and protein. DNA molecules contain the information necessary for constructing and organising cells. Functional segments of DNA are called genes. In a cell which is not dividing, this DNA is present as part of chromatin material. Chromatin material is visible as entangled mass of thread like structures. Whenever the cell is about to divide, the chromatin material gets organised into chromosomes. The nucleus plays a central role in cellular reproduction, the process by which a single cell divides and forms two new cells. It also plays a crucial part, along with the environment, in determining the way the cell will develop and what form it will exhibit at maturity, by directing the chemical activities of the cell. In some organisms like bacteria, the nuclear region of the cell may be poorly defined due to the absence of a nuclear membrane. Such an undefined nuclear region containing only nucleic acids is called a nucleoid. Such organisms, whose cells lack a nuclear membrane, are called prokaryotes (Pro = primitive or primary; karyote ≈ karyon = nucleus). Organisms with cells having a nuclear membrane are called eukaryotes. Prokaryotic cells also lack most of the other cytoplasmic organelles present in eukaryotic cells. Many of the functions of such organelles are also performed by poorly organised parts of the cytoplasm The chlorophyll in photosynthetic prokaryotic bacteria is associated with membranous vesicles (bag like structures) but not with plastids as in eukaryotic cells . When we look at the temporary mounts of onion peel as well as human cheek cells, we can see a large region of each cell enclosed by the cell membrane. This region takes up very little stain. It is called the cytoplasm. The cytoplasm is the fluid content inside the plasma membrane. It also contains many specialised cell organelles. Each of these organelles performs a specific function for the cell. Cell organelles are enclosed by membranes. In prokaryotes, beside the absence of a defined nuclear region, the membrane-bound cell organelles are also absent. On the other hand, the eukaryotic cells have nuclear membrane as well as membrane-enclosed organelles. The significance of membranes can be illustrated with the example of viruses. Viruses lack any membranes and hence do not show characteristics of life until they enter a living body and use its cell machinery to multiply.

• CELL ORGANELLES

Every cell has a membrane around it to keep its own contents separate from the external environment. Large and complex cells, including cells from multicellular organisms, need a lot of chemical activities to support their complicated structure and function. To keep these activities of different kinds separate from each other, these cells use membrane-bound little structures (or ‘organelles’) within themselves. This is one of the features of the eukaryotic cells that distinguish them from prokaryotic cells. Some of these organelles are visible only with an electron microscope.

• ER

The endoplasmic reticulum (ER) is a large network of membrane-bound tubes and sheets. It looks like long tubules or round or oblong bags (vesicles). The ER membrane is similar in structure to the plasma membrane. There are two types of ER– rough endoplasmic reticulum (RER) and smooth endoplasmic reticulum (SER). RER looks rough under a microscope because it has particles called ribosomes attached to its surface. The ribosomes, which are present in all active cells, are the sites of protein manufacture. The manufactured proteins are then sent to various places in the cell depending on need, using the ER. The SER helps in the manufacture of fat molecules, or lipids, important for cell function. Some of these proteins and lipids help in building the cell membrane. This process is known as membrane biogenesis. Some other proteins and lipids function as enzymes and hormones. Although the ER varies greatly in appearance in different cells, it always forms a network system. Thus, one function of the ER is to serve as channels for the transport of materials (especially proteins) between various regions of the cytoplasm or between the cytoplasm and the nucleus. The ER also functions as a cytoplasmic framework providing a surface for some of the biochemical activities of the cell. In the liver cells of the group of animals called vertebrates , SER plays a crucial role in detoxifying many poisons and drugs.

• GOGLI APPARATUS

The Golgi apparatus, first described by Camillo Golgi, consists of a system of membrane-bound vesicles (flattened sacs) arranged approximately parallel to each other in stacks called cisterns. These membranes often have connections with the membranes of ER and therefore constitute another portion of a complex cellular membrane system. The material synthesised near the ER is packaged and dispatched to various targets inside and outside the cell through the Golgi apparatus. Its functions include the storage, modification and packaging of products in vesicles. In some cases, complex sugars may be made from simple sugars in the Golgi apparatus. The Golgi apparatus is also involved in the formation of lysosomes

• LYSOSOMES

Structurally, lysosomes are membrane-bound sacs filled with digestive enzymes. These enzymes are made by RER. Lysosomes are a kind of waste disposal system of the cell. These help to keep the cell clean by digesting any foreign material as well as worn-out cell organelles. Foreign materials entering the cell, such as bacteria or food, as well as old organelles end up in the lysosomes, which break complex substances into simpler substances. Lysosomes are able to do this because they contain powerful digestive enzymes capable of breaking down all organic material. During the disturbance in cellular metabolism, for example, when the cell gets damaged, lysosomes may burst and the enzymes digest their own cell. Therefore, lysosomes are also known as the ‘suicide bags’ of a cell.

• MITOCHONDRIA

Mitochondria are known as the powerhouses of the cell. Mitochondria have two membrane coverings. The outer membrane is porous while the inner membrane is deeply folded. These folds increase surface area for ATPgenerating chemical reactions. The energy required for various chemical activities needed for life is released by mitochondria in the form of ATP (Adenosine triphopshate) molecules. ATP is known as the energy currency of the cell. The body uses energy stored in ATP for making new chemical compounds and for mechanical work. Mitochondria are strange organelles in the sense that they have their own DNA and ribosomes. Therefore, mitochondria are able to make some of their own proteins.

• PLASTIDS

Plastids are present only in plant cells. There are two types of plastids – chromoplasts (coloured plastids) and leucoplasts (white or colourless plastids). Chromoplasts containing the pigment chlorophyll are known as chloroplasts. Chloroplasts are important for photosynthesis in plants. Chloroplasts also contain various yellow or orange pigments in addition to chlorophyll. Leucoplasts are primarily organelles in which materials such as starch, oils and protein granules are stored. The internal organisation of the Chloroplast consists of numerous membrane layers embedded in a material called the stroma. These are similar to mitochondria in external structure. Like the mitochondria, plastids also have their own DNA and ribosomes.

• VACOULES

Vacuoles are storage sacs for solid or liquid contents. Vacuoles are small sized in animal cells while plant cells have very large vacuoles. The central vacuole of some plant cells may occupy 50-90% of the cell volume.In plant cells vacuoles are full of cell sap and provide turgidity and rigidity to the cell. Many substances of importance in the life of the plant cell are stored in vacuoles. These include amino acids, sugars, various organic acids and some proteins. In single-celled organisms like Amoeba, the food vacuole contains the food items that the Amoeba has consumed. In some unicellular organisms, specialised vacuoles also play important roles in expelling excess water and some wastes from the cell.

• CELL DIVISION

New cells are formed in organisms in order to grow, to replace old, dead and injured cells, and to form gametes required for reproduction. The process by which new cells are made is called cell division. There are two main types of cell division: mitosis and meiosis. The process of cell division by which most of the cells divide for growth is called mitosis. In this process, each cell called mother celdivides to form two identical daughter cells (Fig. 5.7). The daughter cells have the same number of chromosomes as mother cell. It helps in growth and repair of tissues in organisms. Specific cells of reproductive organs or tissues in animals and plants divide to form gametes, which after fertilisation give rise to offspring. They divide by a different process called meiosis which involves two consecutive divisions. When a cell divides by meiosis it produces four new cells instead of just two (Fig. 5.8). The new cells only have half the number of chromosomes than that of the mother cells.