1. What are the basic building blocks of DNA and RNA?
sugar + nitrogenous base + phosphate group = nucleotide
2. Which carbons of the deoxyribose sugar link the DNA structure together?
3' and 5' = carbon 3 and carbon 5
3. What is the orientation of the two DNA strands to one another? antiparallel = upside down to eachother
4. Which of the following 3' strands is complementary to this 5' strand: 5' GACCAGTTGCGC 3'
3 CTGGTC
Adenine
Thymine
Box
Phosphate doxyribose backbone
5. What is the name of the structure of DNA formed by two strands of nucleotides?
double helix = 2 DNA strands twisted around eachother
6. Which base is only found in DNA and which base is only found in RNA?
DNA has T (thymine) & RNA has U (uracil)
7. Compare mRNA, rRNA, and tRNA.
mRNA = messenger RNA = carries information from the nucleus to the ribosome
rRNA = ribosome RNA = what ribosomes are made of the nucleolus
Guanine
tRNA = transfer RNA = brings the correct amino acid to the ribosome to make proteins 8. What is semiconservative replication?
semi conservative replication = (old+ new & old + new)
Cytosine
9. What is the function of helicase in replication?
helicase breaks 2 H-bonds (A&T) or 3 H-bonds (C&G) 10. What is the function of Primase in replication?
primase adds RNA primers to begin replication
old1 old2
11. What is the function of DNA Polymerase in replication?
old1
old2
11
new1
new2
DNA polymerase adds complementary bases to build new strands 12. What is the function of Ligase in replication?
Ligase seals the sugar-phosphate backbone
13. What is produced on the lagging strand that does not form on the leading strand?
New leading strands are built continuously. New lagging strands are
14. What is made in the nucleus to pass the DNA code to the ribosome in the cytoplasm?
mRNA is made in the nucleus & exits through the nuclear pores to travel to a ribosome to make pr
15. What changes to eukaryotic pre-mRNA are made to form mature mRNA?
Introns are removes and exons are spliced together. A 5'cap and Poly A-tail are added.
17. What is the mRNA sequence for the template strand DNA sequence: 3' GACAAACTAGAA S'
16. What is a codon?
3 mRNA nucleotides
CUGUUUGAUCUU
18. What does each codon in messenger RNA specify?
3 mRNA nucleotides = codon = one amino acid
C<-->G T->A A--> U
5'CU
19. How many amino acids are on the codon table? there are only 20 amino acids on the codon table
20. How many codons are on the codon table?
There are 64 codes for only 20 amino acids
21. What does the term degenerate mean referring to the codon table?
Some amino acids have more codes than other amino acids
22. What process uses the code carried by messenger RNA for protein synthesis at a ribosome? Replication = DNA--> DNA
Transcription = DNA--> mRNA
Translation = mRNA--? protein
23. Which codons signify the end of translation?
AUG --> Start Codon
UGA, UAG, UAA --> STOP CODONS
24. Which amino acids correspond to the mRNA sequence: GAG-UGC-CCC
Glu-Cys-Pro
mRNA
Amino Acids
25. How is transcription different than translation? Transcription happens in the nucleus where mRNA is
formed from DNA
Translation happens outside the nucleus at a ribosome where mRNA indicated which Amino acids should be put together in what order
First Base in Code Word
U
Second Base in Code Word
G
U
с
An
le
Th-
Lys
Ary
Mal
Th
Se
k
The
An
k
The
Gly
Val
Ala
Gu
Cly
Val
Ala
Ap
Gly
Ala
Arp
Gly
Ala
Stop
"Stop"
ไทย
Sar
"Stop"
Tra
Тут
Cys
Phe
Sar Ser
Cys
Phe
Gh Ag Lou
Gn Arg
Ha Ang Lu
Arg Lou
5 2222
Ser
Pro
Pro
Pro
Pro
333 1122 3!!!
Third Base in Code Word