theme 13

Chapter 11: The Prokaryotes: Domains Bacteria and Archaea

Characteristics of Prokaryotes

  • Prokaryotes are unicellular organisms without a membrane-bound nucleus.

  • They are classified into two domains: Bacteria and Archaea.

Importance of Archaea

  • Acidophilic Archaea: Thrive in acidic conditions, e.g., Sulfolobus, optimal pH ~2, temperature >70°C.

  • Methanogens: Anaerobic archaea producing methane by utilizing hydrogen (H2) and carbon dioxide (CO2); significant in sewage treatment and human microbiota.

Clinical Case Study: Group B Streptococcus (GBS)

  • GBS is part of normal microbiota but can cause serious infections in immunocompromised individuals, especially in neonates.

  • Emerged prominently in the 1970s as a cause of neonatal sepsis.

  • Preventive measures include prenatal screening and administration of antibiotics to carriers.

  • A rare false negative incidence reported.

Microbial Diversity

  • The Earth hosts various prokaryotes, most of which are difficult to cultivate; new identification methods are uncovering their diversity.

  • Size limits exist for prokaryotes due to nutrient absorption constraints.

  • Exploration into the effects of space travel on microbial communities has gained interest.

Discoveries in Microbiology

  • Uncommon organisms like Thiomargarita namibiensis raise questions about cell size limits and metabolic capabilities.


Exploring the Microbiome in Space

Human-Microbe Interaction in Space

  • Potential for microbial contamination from spacecraft may pose risks to other planets.

  • Research on the Astronaut Microbiome Project examines microbial changes during space travel.

  • Bacteria may exhibit different virulence traits in space compared to Earth environments.

Importance of Analysis

  • Studies indicate that certain bacteria, such as Pseudomonas, may form new structures in space cultures that differ from terrestrial counterparts.


The Prokaryotic Groups

Bacteria Classification

  • Bacteria categorized in Bergey’s Manual based on rRNA, morphology, oxygen requirements, etc.

Key Groups of Prokaryotes

  1. Proteobacteria: A diverse group of gram-negative bacteria involved in various ecological roles.

    • Gammaproteobacteria (e.g., Pseudomonas, Escherichia)

    • Epsilonproteobacteria (e.g., Campylobacter, Helicobacter)

  2. Firmicutes: Includes gram-positive bacteria, some with endospore formations (e.g., Bacillus).

  3. Cyanobacteria: Photosynthetic prokaryotes; vital for oxygen production on Earth.

  4. Actinobacteria: Important for antibiotic production and soil health.


Study of Eukaryotic Microorganisms

Fungi, Algae, Protozoa, and Helminths

  • Parasitic diseases affect a significant global population,

  • Fungi and helminths play substantial roles in human health-impacting diseases.

Features of Fungi

  • Predominantly decomposers; some possess pathogenic traits.

  • Key characteristics include hyphae, mycelium structures, and spore reproduction mechanisms.

Protozoa and Helminths

  • Protozoa are unicellular and can reproduce sexually or asexually; they display diverse morphologies.

  • Helminths are multicellular and often exhibit complex life cycles, significantly impacting both human health and agriculture.

Vector Roles in Disease Transmission

  • Arthropods serve as vectors for various pathogens, necessitating strategies for disease prevention.

  • Key examples include the mosquito as a vector for malaria and Culex for West Nile virus.


Diseases Caused by Eukaryotic Microorganisms

Fungal Diseases

  • Eukaryotic pathogens are classified according to their impacts on humans, including mycoses.

  • Extensive discussion on systemic infections and opportunistic pathogens (e.g., Candida albicans).

Eleven Parasites and Their Hosts

  • Critical distinctions between parasitic and free-living organisms, particularly among nematodes and flatworms.

Summary:

  • The microbial world is exceedingly diverse, with prokaryotes (Bacteria and Archaea) and eukaryotes (including fungi, protozoa, and helminths) playing pivotal roles in ecological and health-related contexts, reflecting the intricate relationships between organisms and their environments.