Plant Hormones and Tropisms
PLANT HORMONES
AUXINS
PLANT MOVEMENT: Gravitropism & Phototropism
- Plants exhibit bending or movement in response to stimuli like gravity or light.
- These responses, known as tropisms, are generally slower compared to animals.
- Plants grow either towards a stimulus (positive response) or away from it (negative response).
AUXINS: Chemical Control of Tropisms
- Plants use a growth hormone called auxin to control the direction of root or stem growth in response to stimuli.
- Auxin is primarily produced in the tips of growing stems and roots.
- It diffuses to other areas, moving from a high concentration in the shoot tips to areas of lower concentration.
- Auxin promotes cell elongation (enlargement) in the region behind the tip, known as the meristem.
- Higher auxin concentrations lead to faster elongation and growth.
- Plant growth stimulated by auxin occurs through the enlargement of existing cells, not through cell division to create new cells.
PROCESS OF PHOTOTROPISM
- Phototropism explained: When light comes mostly from one direction, auxin from the tip moves to the shaded side, causing cells there to elongate faster than those on the lit side.
- Unequal growth: This leads to the shoot bending and growing towards the light.
- If light is evenly distributed around the tip, auxin spreads evenly, and the shoot grows straight without bending.
POSITIVE PHOTOTROPISM
- Auxin is synthesized in the tips of the shoots.
- In the presence of directional light, auxin diffuses away from the light towards the shaded side.
- This uneven distribution of auxin results in uneven growth.
- Auxin increases cell growth in the meristem, causing faster elongation on the shaded side compared to the side exposed to light.
POSITIVE GEOTROPISM
- Occurs in roots.
- Higher auxin concentrations result in a lower rate of cell elongation.
- Auxin accumulation on the lower side of the root inhibits cell elongation.
- The lower side grows slower than the upper side, causing the root to bend downwards.
- Advantages: This leads to enhanced access to water and minerals, as well as improved anchorage in the soil.
NEGATIVE GEOTROPISM
- Occurs in shoots.
- When a plant is placed horizontally, auxin diffuses to the lower region of the shoot causing it to bend upwards.
- Advantages:
- Increased access to light and, consequently, increased photosynthesis.
- More access to CO_2, improving the rate of photosynthesis.
- Greater access to O_2, enhancing the rate of respiration.
- Improved access to pollinators.
- Movement away from potential consumers.
GEOTROPISM (Summary)
- Negative Geotropism: Shoot grows away from gravity.
- Positive Geotropism: Root grows towards gravity.
CLINOSTAT
- Definition: A clinostat is a device that slowly rotates plants to negate directional stimuli, such as light and gravity.
- Purpose: Used to study plant growth without external directional influences and serves as a control experiment
CLINOSTAT EXPERIMENT
- Setup A (Control):
- Seedlings are placed in a petri dish with moist cotton wool, positioned on its side in complete darkness.
- Each seedling's radicle (developing root) and plumule (developing shoot) point in different directions.
- Setup B (Clinostat):
- Similar setup to A but placed on a clinostat, rotating to negate gravitropism.
- Observations after 2+ Days:
- Setup A:
- All radicles grew downwards (positive gravitropic response).
- All plumules grew upwards (negative gravitropic response).
- Setup B:
- Radicles and plumules continued to grow in whichever direction they were initially placed.
- The effect of gravitropism was cancelled out by the rotating clinostat.
HOW DO AUXINS WORK? – AN INVESTIGATION
- Experiments demonstrate that auxins can pass through a semipermeable material to cause phototropism.
- If the tip is cut off or blocked by an opaque object, auxins cannot diffuse, thus inhibiting the expected growth response.