BIMM 121 Microbiology Lab Notes
Topics
- What's coming up?
- Introduction to biofilm/microcosm project
- Experimental design
What’s coming up – Math
- WHY math quizzes?????? We will be using math in the lab, starting tomorrow!
- When is the next math quiz?
What's coming up - Math
- How can I improve???
- Week 2 Module
- Material for lab on Wed, Apr 9
- Material for lab on Fri, Apr 11
- Second set of practice dilution problems
What's coming up - Math
- How can I improve???
- Week 3 Module
- Material for lab on Wed, Apr 16
- Materials for lab on Fri, Apr 18
- Third set of practice math problems
What's coming up - Math
- How can I improve???
- Math and dilutions
- Understanding Dilutions
- Guidelines for dilutions
- Dilution Practice problems 1
- Dilution Practice solutions 1
- Video tutorial (50 minutes) on how to do lab math in BIMM 121:
What's coming up - tomorrow
- The following points were shared:
- Wed, Apr 9
- QUIZ on three microscopy videos; MATH QUIZ 1
- Examine liquid cultures from A2
- Examine T-streak plate from A3
- SAVE one streak plate per bench for use in next lab
- BASICS2 - MICROSCOPY with prepared slides; use brightfield
- Fri, Apr 11 MATH QUIZ 2
- B1 set up microcosms for biofilm evolution
- MICROSCOPY - wet mount & phase contrast optics (yeast; Pseudomonas saved from A3, throw away at end of lab)
- You will find a plate of yeast on your bench, for microscopy.
What's coming up - Week 3
- Topic highlighted were:
- Prep for Weds lab, draw out a diagram and/or table of how you will do your dilutions BEFORE
- NO B3 experiment Next week is a heavy week.
What's coming up - Week 3
- Topic highlighted were:
-Week 3 Module
-Material for lab on Wed, Apr 16
-Materials for lab on Fri, Apr 18
-Third set of practice math problems
What's coming up - Week 3
- A research paper needs to be read for Wednesday.
The paper is Nature 1998 paper.
Microcosms - Definition
- Biofilm = consortium of microorganisms in which cells stick to each other and often also to a surface. These adherent cells become embedded within a slimy extracellular matrix that is composed of extracellular polymeric substances, according to Wikipedia
Microcosms - inoculations
- To do experiments follow the below steps:
-Use BEST aseptic technique!!!
-SBW25 Pseudomonas fluorescens bacteria will be used and should be labeled with unique ID number from spreadsheet as follow:
Microcosms Aseptic technique checklist
- The following information should remembered:
-Sterilize workspace with ethanol;
-Uncluttered workspace
-Position Bunsen burner in center of bench
-Adjust flame height Set up workspace according to dominant hand
-Loosen caps before starting
-flame tubes before AND after removing cells
-Keep the tubes and materials sterile Transferred loop is only touched to inoculations.
-Ensure you use sterile tools and sterilize them (flame) after used.
-Complete transfer with graceful movements, be quick to not let materials get contaminated.
-Make sure to sterilize loop after finished . Never give a lid or cap to someone else and Never set a lid or cap on the bench. Always Watch the aseptic technique video AGAIN before your next inoculation . Never switch hands while holding something.
Microcosms - overview
- Show graphical representations of experiment. You should draw your own experiments like this.
Microcosms - Experimental design
- Various conditions and components for setting up your lab are as follows:
-vial condition additive amount of additive bacteria KB total volume
-1 static 10 µL 6 mL 2 static 10 µL 6 mL 3 static 10 µL 6 mL 4 static 10 µL 6 mL 5 static none - 10 µL 5 mL + 990 µL 6 mL
-6 static none - 10 µL 5 mL + 990 µL 6 mL shake a 7 none - 10 µL 5 mL + 990 µL 6 mL 8 shaking none - none 6 mL 6 mL
- experimental vials: controls vials-duplicates.
Microcosms - Experiment B1
- How do you determine a volume that is 2% of the total? Determine this volume in advance of lab!
- If you use H2O2, do NOT exceed 1%.
Static microcosm No additive Shaken microcosm No additive
- To perform experiment note below pointers:
-WHAT do we want to learn?