Photosynthesis Notes
Light Reactions
- Two photosystems:
- Photosystem I: Stroma, absorbs light at 700 nm.
- Photosystem II: Thylakoids, absorbs light at 680 nm.
- Photolysis of water occurs, releasing oxygen.
- Proton gradient generated.
- Synthesis of 6 ATP and 6 NADPH.
Carbon Fixation Reactions (Calvin Cycle/Dark Reaction)
- Does not require light.
- Three main steps: Fixation, Reduction, and Regeneration.
Fixation
- Rubisco enzyme binds three molecules of carbon dioxide (CO2).
- Ribulose 1,5-bisphosphate is converted to three unstable intermediate compounds.
Reduction
- The three unstable intermediates convert to six molecules of glyceraldehyde-3-phosphate (PGAL).
- 6 ATP are consumed (hydrolyzed to ADP and inorganic phosphate).
- 6 NADPH are consumed (converted to NADP+).
Regeneration
- One molecule of PGAL is used to synthesize sugars, starch, amino acids, and fatty acids.
- The remaining five PGAL molecules regenerate ribulose 1,5-bisphosphate, requiring an additional 3 ATP.
ATP Consumption
- Total of 9 ATP are consumed in the Calvin cycle (6+3).