Chapter 4 Hostology

  • 4.1 Introductions to Tissues 

    • Tissue- group of structurally and functionally related calls and their external environment that together perform common functions (chemical->cellular->Tissue)

    • Histology- the study of the normal structure of tissues 

      • two basic components

        • a discrete population of cells that are related in structure and function.

        • a surrounding material called the Extracellular Matrix, differs in composition in each tissue type.

  • 4 primary tissue types

    • Epithelial tissues (4)- sheets of tightly packed cells with little ECM. 

      • covers and lines body surfaces and cavities and form parts of gland 

    • Connective Tissue (4)- connects all other tissues together

      • cells are scattered through the large amount of ECM

      • bind, support, protect, and allow transport of substances

    • Muscular Tissue (3)- cells contract and generate force; little ECM

    • Nervous Tissue (2)- cells (neurons) generate, send , and receive messages: including cells that support the neurons with a unique ECM

  • Cell Junction- connections of neighboring cells in a tissue linked to one another by integral proteins 

    • Three types:

      • Tight junctions( occluding)- are like a zipper, compose of integral or “locking” proteins 

        • Prevents passage of macromolecules, although some are leaky and doen provide a complete seal

      • Desmosomes- like a button, composer linker proteins in adjacent plasma membranes

        • distribute mechanical stress

        • can develop antibodies

      • Gap Junctions- small pores made or protein channels in adjacent plasma membranes

        • allows small substance to pass freely

  • 4.2 Epithelial Tissue- found on every external and internal body surface, so acts as a barrier between the body and the environment

    • Covering/ lining epithelia

    • Glandular epithelia

    • 5 Functions of Epithelial

      • Protection- continuous surface that shields underlying tissue from mechanical & thermal injury, produces keratin: undergoes mitosis rapidly & frequently.

      • Immune Defenses- cells of immune system scattered throughout epithelial tissues

      • Secretion- form glands that produce substances such as sweet, oil, and hormones

      • Transport into other tissues- selectively permeable barriers that allow certain substances to pass by passive or active transport

      • Sensation- most epithelial supplied with nerves that detect changes in internal & external environment, specialized epithelial. cells are responsible for some sensations 

  • Components and classification of epithelia

    • Components (sides) of epithelia

      • have 1 side in contact with the extracellular space ( apical Surface), 1 in contact with deeper cells or the basal lamina (basal surface) and 2 sides 

    • Classification of Epithelia: layers and shape

      • 3 possible layers

      • Simple Epithelia- single layers of cells

      • Stratified epithelia- more than one layer of cells 

      • Pseudostratified epithelia- single layer of cells that appears to be multilayered 

      • 3 possible shapes 

      • Squamous Cells- flattened cells 

      • Cuboidal Cells - short cells

      • Columnar Cells- tall and elongated cells  

  • Combining layers and shapes

    • Simple epithelia layering- one cell layer, 

      • no protection

      • lines hollow organs and surfaces where diffusion or transport occurs

        • includes:

        • simple squamous epithelia

          • single layer of flat cells

          • resemble fried eggs

          • diffuse occurs across cells 

          • found  in air sacs of lungs, serous membranes, and lining of blood

        • simple cuboidal 

          • single layer of cube shaped cells 

          • appear large with a central nucleus 

          • diffusion occurs and some secret substances

          • found in kidney tubules and glands

        • Simple columnar epithelia

          • single layer of tall cells

          • appear rectangular

          • some have cilia

          • some produce secretion

          • found in the small intestine, uterine tube, kidney tubules and glands

        • pseudostratified columnar

          • single layer of of cells the appears stratified

          • most are ciliates

          • includes goblet cells that secrete mucus

          • found in respiratory passages and nasal cavities for protection 

  • other type of layer/ shape combo is 

    • Stratified epithelia layering- 

      • thicker than simple epithelia

      • protective barriers

      • common in areas of high stress

      • cell shape changes throughout thickness of tissue

      • Types

        • Stratified Squamous Epithelial- Most widespread!

          • Keratinized- outer layer of skin

          • nonkeratinized

        • transitional epithelia (stretchy): cuboidal or Squamous?

        • others

          • stratified cuboidal epithelia( small amount 

          • Stratified columnar epithelia (small amount)

  • Covering and lining epithelia

    • Stratified squamous epithelia 

      • keratinized- outer layer of skin

      • nonkeratinized has distinct nucleated cells on apical surface

      • found in epithelium of the mouth, pharynx, esophagus, anud and vagina

    • Stratified cuboidal: has several layers of cuboidal epithelial cells and the underlying basal lamina; lines the ducts of certain exocrine glands (sweat glands)

    • Stratified columnar: Several layers of cuboidal epithelial cells and the underlying basal lamina; lines the ducts of certain exocrine glands (Salivary glands as well as part of the urethra and the conjunctive ( the thin, clear membrane lining the anterior surface of part of the eye and the inner eyelid)

      • • These are both rare in the human body 

  • • Transitional Epithelium: The final part has several layers 

  • of epithelial cells and the underlying basal lamina found in the organs of the urinary system; cells can change shape. It lines the interior kidney, the ureter the urinary bladder, and the urethra 

  • Glandular Epithelia

    • A gland is a structure that makes and secretes a product

    • arise from epithelial tissue that grew inward into the underlying connective tissue rather than remaining at the surface

  • Secretory cells: manufacture a product and release it 

    • classified by the number of cells, shapes, and how they release product 

    • Glands releases its products in two ways 

      • Exocrine Glands:(A gland that secretes a product through a duct to the external surface of the body or into the respiratory and gastrointestinal)

        • releases secretions to the apical surface of the epithelium generally to the exterior of the boyd and into a hollow organ that opens to the outside of the body

        • The secretions have local actions only- which means they only affect the cells in their general vicinity.

        • The simplest exocrine glands are the

      • Unicellular glands

        • The most common unicellular gland in the body is the Goblet cell:(Secretes mucus) found in the epithelium lining the digestive and respiratory tracts.

          • secrete mucus,  a thick, sticky liquid that protects the underlying epithelium 

      • Multicellular exocrine glands: made of clusters of secretory cells arranged in different ways.

      • Simple glands: have ducts that dont branch whereas compound glands do have branches 

        • Arranged in three shapes 

          • tubular: long and straight or coiled

          • Acinar: spherical

        • Tubuloacinar: both tubular and acinar portions 

      • Types of exocrine secretion 

        • Merocrine secretion: (within the wall) a type of secretion in which the product is secreted by exocytosis

        • Holocrine: ( outside the wall) a type of exocrine secretion in which the product accumulates in the cell until the cell ruptures and dies 

          • accumulates in the cytosol, and the product isn't released until the cell ruptures and dies 

        • Apocrine Secretion: rare: a portion of the cytoplasm is pinched off with the product being secreted

  •     Endocrine Glands: Lack ducts that secrete their products which are usually hormones directly into the blood.

  •     4.3 Connective Tissues 

    • Connective tissues: a type of tissue characterized by extensive extracellular matrix

      • functions in support, protection, and transport

      • some more functions

        • connecting and binding: connect structures in the body

          • in organs they bind other tissues layers together

          • can be found between organs, they anchor them in place

        • Support: certain connective tissues such as bone and cartilage support the weight of the body 

        • Protection: the bone tissue protects certain internal organs  and the fat tissue provides shock absorption 

        • Transport: blood is fluid connective tissue that is the main transport medium in the body 

      • Two basic types of connective tissue:

        • Connective tissue proper: A type of connective tissue that generally functions to connect and support other tissues, widely distributed in the body, which connects tissues and organs

          • also called general connective tissue

          • widely distributed in the body 

          • five cells of the connective tissue proper

            • Fibroblasts: a cell type within connective tissue proper that produces components of the extracellular matrix

              • they produce fibers as well as ground substance and other elements of the ECM

              • lie close to collagen fibers and they continuously produce collagen proteins 

            • Adipocytes: also called fat cells, are another type of resident cell found in many different connective tissues, the main type of cells in the adipose tissue 

              • found in the cytoplasm 

              • nuclei are squished to the perimeter of the cell

            • Mast cells: are the largest resident cells in the connective tissues

              • cells of the immune system 

              • have granules or cytosolic inclusions 

            • Phagocytes: cells of the immune system that can ingest foreign substances, microorganisms, and dead and damaged cells by phagocytosis

              • two common types 

              • Macrophages: which can be either resident or migrant cells in connective tissues

              • Neutrophils: migrant immune cells 

          • Types of connective Tissue Proper

          •  connective: also known as the areolar connective tissue,

          •  contains a ground substance with protein fibers, fibroblasts,

            • jelly-like

            • found in the deep epithelium

            • supports and houses blood vessels and organs

            • most widespread throughout the body

            • Dense -3 types of tissue

            • Dense irregular Connective tissue 

              • composed of  protein fibers with mostly collagen fibers

              • arranged haphazardly

              • strong tissue that resists tension in all 3 planes 

              • found in the dermis and around organs and joints 

            • Dense Regular collagenous Connective Tissue 

              • contains thick collagen fibers arranged in parallel bundles 

              • resists tension in one direction

              • found in tendons ( which join muscle to the bone )and ligaments (which join bone to bone)

            • Dense Regular Elastic connective tissue 

              • consists of mostly parallel elastic fibers with randomly oriented collagen fibers

              • allows organs to stretch 

              • Found in the lining of the large blood vessels & some ligaments                       

            •  Reticular Tissue 

              • includes numerous reticular fibers produced by supporting fibroblasts 

              • Form fine, mesh like networks for support & weblike nets that trap foreign cells; forms the basement membrane       

            • Adipose tissue 

              • fat  tissue consisting of adipocytes, surrounding fibroblasts & ECM

              • can increase in size

            • functions insulation, warmth, shock absorption protection and energy reserve, found deep to skin in hypodermis

              • adipose tissue has two types White adipose and brown adipose 

                • White Adipose: accumulates in the abdomen, breast , hip, buttocks and thighs, heart and abdominal organs

                • Brown adipose( we lose when wwe become adults), provide heat protectant like a tempostat

  • 4.3 three specialized connective Tissues: cartilage, Bone, and Blood

    •  Cartilage- 3 types Hyaline( most common, lines bones and joints) Fibrocartilage, Elastic( uncommon, ear, throat, and small intestine)

      • cartilages is tough, flexible tissue that absorbs shock, resistant to tension, compression and shearing forces

      • usually surrounded by outer sheath call perichondrium (made of dense irregular connective tissue, supplies nourishment  from blood products to the cartilage 

      • Cartilage is Avascular

        • Two cell types in cartilage 

        • Chondroblasts- immature cartilage cells 

        • Chondrocytes- mature cartilage cell, housed in cavities called Lacunae

      • Hyaline Cartilage

        • most common

        • ECM is ground substance with a fine collagen that forms small bundles; 

        • Covers ends of bone , rib attachment sights to sternum and nose and respiratory tract

        • makes up most of the skeleton

      • Fibrocartilage

        • ECM is filled with bundles of collagen and some elastic fibers made from fibroblasts

        • found in fibrous joints, including the intervertebral discs and other articular discs that improve the fit of 2 bones

        • lacks a perichondrium

        • examples: labrum ( hips and shoulders)

      • Elastic Cartilage 

        • ECM is filled with elastic fibers 

        • found in the external ear (pinna)and parts if the larynx

        • Tissue vibration and assists in detection of sound in the air and production of sound in the larynx

        • Uncommon

    • Bone

      • Functions 

        • suport

        • protection

        • attachment site for muscles 

        • storage ( fat, calcium)

        • Houses bone marrow 

      • Bone ECM consists of:

      • Organic portion

        • 35% of bone mass is made of collagen fibers and osteo ground substance 

      • Inorganic portion 

        • 655 of bone mass, made of calcium phosphate crystals( Hard)

    • Bone Cells 

      • Osteoblasts- Immature,

        • Carry out bone deposition 

        • synthesize organic portion and produce chemicals requires for calcium salts to deposit in the ECM

      • Osteocytes- Mature osteoblasts become this when surrounded by the ECM

        • immobile 

        • live in lacune 

        • produce substances for bone maintenance

      •  Osteoclasts- multinucleated cells that carry out bone resorption ( break down of bone)

        • secrete hydrogen ions and enzymes to break down the ECM

    • Bone Remodeling- Bone deposition and bone resorption are constantly occurring in healthy bone, depending on type of stress placed

  • Blood- functions is transport, The bloods Ecm is plasma; contains water, dissolved solutes, and globular proteins 

    • Cells of blood

      • Erythrocytes- RBC oxygen carriers

      • Leukocytes- WBC 

      • Platelets- Blood clotting

  • 4.4 Muscle Tissues

    • Muscle cells ( Myocytes)- excitable cells that respond to electrical or chemical stimulation

      • Cytoplasm is filled with proteins called Myofilaments, including the contractile proteins myosin and actin, surrounding the endomysium

      • Striated muscle cells - filaments are organized into regions that produce dark and light areas calle “band”s or striations

      • Smooth muscle cells - myofilaments are in irregular bundles scattered in the cytoplasm, NO STRIATIONS

        • Hypertrophy( increase) and atrophy (decrease) 

    • Skeletal Muscles- attached to the skeleton to produce body movement 

      • controlled by the nervous system 

      • Voluntary movement

      • formed by fusion of embryonic myoblasts causes large multinucleate cells also called muscle fibers.

    • Cardiac Muscle- Only in the heart 

      • involuntary contractions 

      • short with branches and a single nucleus 

      • intercalated disks 

      • contains gap junction and tight junctions 

      • found between cells and permit heart muscle to contract as a unit

    • Smooth muscle- found in walls of hollow organs, blood vessels, the eyes, skin , and ducts of some glands

      • involuntary

      • cells are flattened, single nucleus, gap junctions in the plasma membrane connect cells to other smooth muscle cells. 

      • example: Gi tract

    • Nervous tissue- makes up majority if the brain, spinal cord, and nerves 

      • ecm contains protein fibers, and mostly ground substance, wit unique proteoglycans 

        • Two types of cells 

        • Neuroglial Cell- supportive cells that anchor neurons and blood vessels in place, monitor the compression of the ECF, speed up the rate of nerve impulse transmission and circulate fluid around the brain and spinal cord

          • can divide by mitosis

        • Neurons - generate, conduct and receive nerve impulses

          • structure includes:

          • Cell body: large center with nucleus and organelles

          • Axon: moves impulse to target cell

          • Dendrites: arms that receive messages

          • Neurons are Amitotic ( you don't get new ones)

  • 4.6 Putting it all together: the big picture of Tissues in organs 

  • 4.7 Membranes 

    •  membrane- thin sheet of one or more tissues that lines a body surface or cavity 

      • consists of superficial layer of secretory cells and a layer of connective tissue 

      • some have smooth muscle 

      • anchors organs in place

      • serves as a barrier

      • function in immunity 

      • secretes various substances 

      • four general types of membranes

        • 2 true membranes

          • serous 

          • synovial

        • 2 “membrane- like “ structures

          • mucous

          • cutaneous

    • True membranes- fit the structural and functional definitions of a membrane

      • DO NOT open outside of the body

      • Serous membrane- line the pleural, pericardial, and peritoneal body cavities

        • consists of simple squamous epithelial tissue called mesothelium 

        • outer lays is visceral layer

        • mesothelial cells produce Serous fluid which lubricates organs 

      • Synovial Membranes- line cavities surrounding freely moveable joints, including the hip, knee, elbow, and shoulder

        • made of two connective tissue layers

          • inner- consists of modified fibroblasts called synoviocytes- secret synovial fluid that lubricates the joints 

          • External— a mixture of loose and dense irregular connective tissue

      • Membrane-Like Structures- don’t completely fit the structural and functional definitions of a membrane: DO OPEN to the outside of the body 

        • Mucous membranes- lines the body passages as part of walls of hollow organs that open to the outside of the body

          • consists of a epithelium, basement membrane, and a layer oif connective tissue called lamina prpria and sometimes smooth muscle

          • glands with goblet cells that secrete mucus 

        • Cutaneous membrane - the skin, which is the largest organ 

          • open to the environment 

  • 4.8 Tissue repair 

    • process of wound healing, occurs differently in different tissue & is dependant on tissue ability to regenerate 

    • 2 possible process of healing

      • Regeneration- damage or dead cells are replaced with cells of the same type

        • this is a good thing 

      • Fibrosis- fibroblasts divide by mitosis and produce Collagen to fill in defect left by injury

        • results in scar tissue, which is dense irregular connective tissue 

        • not good or strong 

  • Capacity of specific tissues for tissue repair 

    • Regeneration- or fibrosis occurring in tissue largely determined by ability of tissue to undergo mitosis

    • Tissue types and their mode of healing

      • Epithelial tissues-

        • undergo regeneration 

        • many contain immature stem cells; divide continually to replace dead, injured, or worn-out cells 

      • Connective Tissue 

        • most heal by regeneration 

        • cartilage is an exception and often heals by FIBROSIS

      • Smooth muscle Tissue-

        • Usually regeneration

        • cells retain their ability to undergo mitosis

      • Cardiac and Skeletal Muscle

        • generally heal by Fibrosis

        • cardiac and skeletal muscle cell size and complexity prevent mitosis

        • satellite cell in skeletal muscle tissue can divide and become skeletal muscle cells which provides a limited degree of regeneration 

      • Neurons of nervous Tissue 

        • Do not regenerate

  • Other factors of tissue repair 

    • nutrition and blood supply- important determines of tissues repair

      • nutrition- collagen is needed in tissue repair, so protein intake and vitamin c (needed soe fibroblasts to make collagen) must be considered

      • Blood supply- adequate blood flow to injured areas is also important 

        • people suffering from disease of the blood vessels may have non healing wounds, even in tissues capable of regeneration