neural tube defects

Lecturette 2: Neural Tube Defects


Learning Objectives:

  • Describe the pathophysiology of anencephaly and spina bifida.

  • Describe the three major subtypes of spina bifida.

  • Explain common treatments and management approaches for spina bifida

  • Explain the public health management of an identified risk factor for spina bifida




Neural Tube Defects — Extremely Detailed Lecture Notes


Lecture introduction

  • Lecturer: Kay Double

  • Lecture topic: Neural tube defects

  • This lecture links to unit themes including:

    • Neural development

    • Vulnerable populations

    • Disease prevention

  • It is also linked to:

    • Wet anatomy lab class

  • The lecture also provides a chance to discuss:

    • Health at the population level

    • An Australian public health initiative

Definition of public health given in the lecture

Public health is:

  • “the art and science of preventing disease, prolonging life and promoting health through the organised efforts of society”

  • This definition was stated as being from the World Health Organization

Content warning

  • The lecturer warns that the lecture contains:

    • graphic medical images

  • Students with concerns are told to:

    • email the MEDS2001 coordination team

    • arrange a suitable replacement


Learning outcomes

By the end of the lecture, you should be able to:

  1. Describe the pathophysiology of:

    • anencephaly

    • spina bifida

  2. Describe the three major subtypes of:

    • spina bifida

  3. Explain common treatments and management approaches for:

    • spina bifida

  4. Explain a public health management approach for:

    • an identified risk factor for spina bifida


What are neural tube defects?

Definition

  • Neural tube defects are:

    • a group of disorders

    • caused by errors in the very early development of the neural tube

    • during the first month of life in utero

Prevalence

  • Global prevalence is about:

    • 1 in 1000 births


Normal neural tube development

  • The lecture refers back to earlier lectures from Associate Professor Kathy Luu/Leamy on neural tube development.

  • The neural tube forms by:

    • closure of the tube

  • Closure begins:

    • in the middle of the neural tube

  • Then proceeds in both directions:

    • rostrally (towards the head)

    • caudally (towards the tail/lower end)

This normal closure process is critical because failure of closure at different ends causes different neural tube defects.


Failure of rostral neural tube closure: Anencephaly

What happens?

  • If the rostral neural tube fails to close properly:

    • a severe disorder called anencephaly results

Meaning

  • Anencephaly literally means:

    • “no brain”

Consequence

  • The brain does not develop properly

  • It is described as:

    • a fatal condition

Outcome

  • A fetus with anencephaly usually:

    • dies in utero

    • or in the first few days after birth


Failure of caudal neural tube closure (more common): Spina bifida

What happens?

  • If the caudal neural tube fails to close:

    • the condition is called spina bifida

Meaning of the term

  • Spina bifida comes from Latin and means:

    • “split spine”

  • This refers to:

    • a split down the spine

    • or failure of the neural tube/spinal structures to close properly


Spina bifida — overview

Definition

  • Spina bifida is a:

    • congenital disorder

  • Congenital means:

    • present from birth

Developmental basis

It arises when there is failure of development of:

  • the vertebral arches

  • during development of the vertebral column

  • as well as abnormal development of:

    • the spinal cord

    • associated nerves

    • associated muscles

Visible defect

  • A cyst-like sac may protrude from the spine

  • The spinal defect may be:

    • open to the environment

    • or closed, covered by skin

Worldwide burden

  • The lecturer states that about:

    • 215,000 to 322,000 pregnancies worldwide

    • are affected by spina bifida


Major subtypes of spina bifida

The lecture describes three major subtypes:

  1. Spina bifida occulta (mildest form)

  2. Meningocele

  3. Myelomeningocele


1. Spina bifida occulta

Severity

  • This is the mildest form of spina bifida

Structural defect

  • One or more vertebrae do not close properly

  • Usually around:

    • L5

    • S1

    • lower lumbar / sacral region

Key feature

  • There is no protrusion of the spinal cord through the vertebral column

External sign: cutaneous stigmata

Although the defect is internal, there may be visible skin signs over the lesion level:

  • called cutaneous stigmata

Typical appearance may include:

  • differently coloured skin

  • a small dimple

  • a patch of hair growth

These skin changes can indicate an underlying spinal lesion.

Internal associated abnormalities


Spina bifida occulta may be associated with abnormalities inside the vertebral canal, including:

Syringomyelia

  • A cyst in the spinal cord

  • This can:

    • place pressure on the developing spinal cord

    • damage spinal cord tissue

Tethered cord syndrome

  • The spinal cord is abnormally connected to the sides of the vertebral column

  • This can cause:

    • abnormal movement of the spinal cord

    • spinal cord damage

Symptoms and effects

These associated abnormalities can lead to a range of symptoms, including failure of normal development of associated systems.

Possible problems include:

  • anorectal problems

  • urogenital malformations

  • bowel dysfunction

  • bladder dysfunction

  • sexual dysfunction

Important point

  • In very mild cases, spina bifida occulta may be:

    • completely asymptomatic

  • Some people may not even know they have it


2. Meningocele

Severity

  • More serious than occulta

What protrudes?

  • A sac-like cyst of the meninges protrudes through the vertebral defect

Recall: meninges

  • The meninges are the membranes covering:

    • the brain

    • the spinal cord

  • They occur in three layers

Contents of the sac

  • The protruding meningeal sac contains:

    • cerebrospinal fluid (CSF)

Important distinction

  • Usually there is no spinal cord tissue in the cyst

  • It is mainly:

    • a meningeal protrusion filled with CSF

Associated problem

  • There are often spinal abnormalities at that lesion level


3. Myelomeningocele

Severity

  • This is the most severe

  • Also described as the most common form of spina bifida

What protrudes?

Both of the following protrude through the vertebral defect:

  • meninges

  • spinal cord tissue

Usual location

  • About 80% occur in:

    • the lower back

    • especially the lumbar and sacral regions

Why it is severe

  • The spinal cord itself is squeezed into the meningeal sac

  • Therefore there is direct involvement of:

    • neural tissue

  • This causes:

    • damage to spinal cord tissue

Associated symptoms

  • paralysis of limbs, especially:

    • lower limbs

  • paralysis/weakness of:

    • abdominal muscles

  • loss of sensation

  • loss of movement

  • incontinence

Management

  • If diagnosed in time:

    • surgery is usually required

  • Timing:

    • either before birth

    • or very soon after birth

Visual clue noted by lecturer

  • The lecturer notes the baby shown appears to have a very large head

  • This leads into discussion of complications such as hydrocephalus


Complications associated with myelomeningocele

A major complication discussed is:

Chiari II malformation

What happens?

  • Part of the lower brain:

    • cerebellum

    • brainstem

  • instead of remaining inside the skull,

  • protrudes through the:

    • foramen magnum

  • This is the large hole at the base of the skull

Direction of protrusion

  • These lower brain structures descend down toward:

    • what would be the vertebral column

Consequence

  • This protruding tissue may become:

    • compressed

    • damaged

Effects

This can cause a range of symptoms including:

  • sensory changes

  • motor symptoms

  • minor symptoms progressing to paralysis

  • brainstem damage

  • complications associated with brainstem dysfunction

  • Hydrocephalus (very serious)


Hydrocephalus

Meaning

  • Hydrocephalus means:

    • “water head”

Mechanism

Normally:

  • CSF flows through:

    • the ventricles of the brain

    • and down through the centre of the spinal cord

In hydrocephalus:

  • this flow becomes obstructed

Result

  • The ventricles enlarge due to accumulation of CSF

  • Increased fluid pressure causes:

    • compression of brain tissue against the hard skull

Effect on skull/head

  • In infants, the head enlarges

  • This produces the characteristic:

    • large head size

Why it is serious

  • It is a very serious condition

  • Outcomes depend on:

    • severity of pressure

    • extent of brain tissue compression

    • effectiveness of treatment

    • timing of treatment

Treatment

  • May require surgery

  • A shunt may be inserted to:

    • drain excess CSF from the ventricles

  • This may even be done:

    • while the child is in utero

Neurological outcome

  • Depends on:

    • how severe the hydrocephalus is

    • how effective treatment is

    • how early treatment occurs


Diagnosis of spina bifida

Prenatal diagnosis

In many cases, spina bifida is diagnosed:

  • in utero

Method 1: Ultrasound

  • First trimester ultrasound may show whether:

    • the neural tube is developing normally

    • the brain is developing normally

  • If something appears wrong:

    • further confirmation is often done by

    • second trimester ultrasound

    • when development is more advanced and clearer

Method 2: Maternal serum alpha-fetoprotein

  • A protein called alpha-fetoprotein (AFP) is mentioned

  • High levels in the mother’s serum during early pregnancy indicate:

    • increased risk of spina bifida

Therefore AFP can be used as:

  • a biomarker


Symptoms and functional outcomes of spina bifida

The lecture emphasizes that symptoms are very variable.

What determines severity?

Symptoms depend on:

  1. level of the spinal cord lesion

  2. severity of the lesion

Possible effects

  • paralysis

  • numbness

  • tingling

  • bladder dysfunction

  • bowel dysfunction

  • sexual dysfunction

  • cognitive dysfunction

Important point

  • Cognitive dysfunction is said to be:

    • very common in people with spina bifida


Treatment and long-term management

Cure?

  • There is no cure for spina bifida

Main aim of management

  • manage symptoms

  • maintain quality of life

Important principle

Management usually requires a:

  • multidisciplinary care team

This may include multiple:

  • medical specialists

  • allied health specialists

This is important because spina bifida is often:

  • a complex

  • long-term

  • lifelong
    health condition


Prognosis / life expectancy / long-term outcomes

Quality of life

  • People with spina bifida can now often have:

    • a reasonable

    • even good
      quality of life

  • especially if well managed

Survival

  • Mean survival is now around:

    • 50 years

Common causes of death

Death usually occurs due to complications of:

  • CNS dysfunction

  • sepsis

  • cancer

Support needs

Because the condition is:

  • lifelong

  • complex

it is important to support:

  • the patient

  • and also the caregivers

The lecture stresses that caregivers need:

  • long-term support


Causes of spina bifida

General cause

  • Mostly:

    • sporadic

    • idiopathic

  • meaning the cause is not fully known in most cases

Genetic contribution

  • There is likely:

    • a genetic predisposition

  • Supported by:

    • increased prevalence in some populations in different parts of the world

Environmental and lifestyle contribution

The lecture says lifestyle and environmental factors also play a role.


Risk factors for spina bifida

Maternal health

  • diabetes

  • obesity

Drug exposure

  • especially valproate

Valproate

  • Used for:

    • epilepsy

    • bipolar disorder

    • prevention of migraines

Other substance exposure

  • abuse of other substances can increase risk

Environmental exposures

  • air pollution

Nutrition

  • poor nutrition

Alcohol and smoking

  • both are risk factors


Folate and neural tube development

Key nutrient

  • Folate = vitamin B9 (soluble)

Why it matters

  • Required for:

    • normal development of the brain and spinal cord

  • Particularly critical for:

    • normal closure of the neural tube

When is folate most important?

  • During weeks 3 to 6 of embryogenesis

  • This is very early pregnancy

  • Often:

    • before the mother even knows she is pregnant

So adequate folate must be available very early, not just later in pregnancy.


Dietary sources of folate

Foods mentioned as containing folate:

  • green leafy vegetables

  • liver

  • nuts

  • avocados

  • lentils

  • chickpeas

Practical issue

  • In some populations, these foods may be:

    • hard to obtain

    • expensive to obtain

Alternative: take a supplement


Folic acid supplementation

Difference between folate and folic acid

  • The supplement given is not folate itself

  • It is:

    • folic acid

  • Folic acid is described as:

    • a prodrug

  • It can be:

    • manufactured/synthesised

  • Then converted in the body to:

    • folate

Recommended dose

For women considering pregnancy:

  • recommended folic acid intake is about:

    • 400–800 micrograms per day

  • taken:

    • before pregnancy

    • and during pregnancy


Public health intervention: United States

Historical development

  • In the 1990s, folate was discovered to be critical for neural tube closure

  • In 1998, the US Food and Drug Administration decided to:

    • fortify some grain and cereal products with folic acid

Purpose

  • To ensure the community/population received adequate folic acid

Outcome

  • Following fortification:

    • spina bifida rates in the US reduced by 23%

Importance

  • This showed that a:

    • community-wide public health initiative

    • could significantly reduce neural tube defects


Public health intervention: Australia

Policy

  • In 2009, the Australia New Zealand Food Standards Code mandated that:

    • commercial producers of bread

    • must use flour supplemented with folic acid

Amount added

  • Flour must contain:

    • 2–3 mg folic acid per kg of flour

What this means nutritionally

  • About 100 g of bread (around 2 slices) gives about:

    • half the recommended daily intake of folate

The lecture says the daily intake is about:

  • 0.2 mg/day
    although the exact numbers were stated as not essential to remember.

Key point the lecturer wants remembered

You do not need to memorise the exact numbers.

What matters is:

  • In Australia, it is mandated by law

  • Commercial bakers must use flour with added folic acid


Australian evidence of effectiveness

2019 analysis

  • In 2019, researchers from SA Health analysed:

    • benefits versus costs

    • using Australian population data

Findings

They concluded the intervention was:

  • low cost

  • resulted in 32 fewer neural tube defect cases per year

  • saved about $1.5 million per year in the health system

Equity benefit

A very important conclusion was that it improved:

  • equity

Especially in vulnerable groups such as:

  • teenage mothers

  • Aboriginal and Torres Strait Islander mothers

Overall conclusion

This simple intervention:

  • adding small amounts of folic acid to commercial baker’s flour

  • produced a net benefit for society

  • in a:

    • low-cost

    • efficient
      manner


Big-picture ideas from the lecture

1. Neural tube defects happen very early

  • They arise during the first month in utero

  • This is why prevention has to occur before or very early in pregnancy

2. Site of failed closure determines the defect

  • Rostral failure → anencephaly

  • Caudal failure → spina bifida

3. Spina bifida is not one single presentation

  • It ranges from:

    • very mild and asymptomatic

    • to severe disability with CNS complications

4. Symptoms depend on lesion level and severity

  • Functional outcomes vary greatly

5. There is no cure

  • Management focuses on:

    • symptom control

    • surgery where needed

    • preserving quality of life

    • multidisciplinary care

6. Folate is a major preventable risk factor

  • Adequate folate in early pregnancy is crucial

7. Public health policy can prevent disease

  • Food fortification with folic acid is a strong example of:

    • effective public health

    • disease prevention at the population level

    • improved equity in vulnerable groups


Quick exam-style summary

Neural tube defects

  • Group of disorders caused by failure of normal neural tube closure in early embryonic development.

Anencephaly

  • Due to failure of rostral neural tube closure.

  • Fatal because the brain does not develop properly.

Spina bifida

  • Due to failure of caudal neural tube closure.

  • Congenital defect involving abnormal vertebral arch/spinal cord development.

Three major subtypes

  • Occulta: vertebral defect only, no cord protrusion, may be asymptomatic

  • Meningocele: meninges + CSF protrude

  • Myelomeningocele: meninges + spinal cord tissue protrude; most severe/common

Important complications

  • Chiari malformation

  • Hydrocephalus

  • Motor, sensory, bladder, bowel, sexual, and cognitive dysfunction

Diagnosis

  • Prenatal ultrasound

  • Maternal serum alpha-fetoprotein

Treatment

  • No cure

  • Surgery in some cases

  • multidisciplinary long-term management

Risk factors

  • Genetic predisposition

  • maternal diabetes/obesity

  • valproate

  • alcohol

  • smoking

  • air pollution

  • poor nutrition

  • folate deficiency

Prevention

  • folic acid supplementation

  • folic acid fortification of flour/bread

  • proven public health benefit in US and Australia