MCQ test

Physiology Definition

  • Physiology is the investigation of the processes or functions of living things.

Why Study Physiology?

  • It provides an in-depth understanding of the human body and how it functions.

Organizational Levels of the Human Body

  1. Chemical Level
    • Involves combinations of molecules.
  2. Cell Level
    • Cells are the structural and functional units of the body.
    • They contain organelles, which are "little organs" performing specific functions.
  3. Tissue Level
    • Tissues are comprised of similar types of cells.
    • There are four main types: epithelial, connective, muscle, and nervous.
  4. Organ Level
    • Organs are composed of two or more types of tissues.
  5. Organ System Level
    • Multiple organs work together to perform a common function.
    • There are 11 human body organ systems.
  6. Organism Level
    • The organism is a whole, living being.

Characteristics of Life

  • Organisation: The interrelation of organizational levels to function.
  • Metabolism: Chemical reactions that maintain life. Involves the use of energy to perform functions.
  • Responsiveness: The ability to read internal or external changes and respond or adjust to stimuli. Control systems include the nervous and endocrine systems.
  • Reproduction: Creation of new cells.
  • Development: Changes over time, from fertilization to death. Includes differentiation, where immature cells change to mature, specialized cells.
  • Growth: Increase in cell size and/or number.

Cell Biology

  • Key components include the plasma membrane and the nucleus.

Cell Functions

  1. Cell Metabolism and Energy Use
    • Chemical reactions within cells often involve energy transfer.
  2. Synthesis of Molecules
  3. Communication
    • Chemical and electrical communication between cells.
  4. Reproduction and Inheritance
    • Cells contain DNA molecules, which carry an organism's genetic information.

Plasma Membrane

  • Functions:
    1. Barrier separating intracellular fluid (ICF) from extracellular fluid (ECF).
    2. Encloses cell contents.
    3. Attaches cells to the extracellular area.
    4. Enables communication.
    5. Facilitates movement into and out of the cell.

Plasma Membrane Components

  • Membrane Lipids
    • Phospholipids form a lipid bilayer.
    • Cholesterol is also present.
  • Membrane Proteins
    • Integral membrane proteins
    • Peripheral membrane proteins

Plasma Membrane Movement

  • The plasma membrane is selectively permeable.
  • Nutrients need to move into the cell, and waste needs to move out.
  • Two types of movement:
    • Passive transport
    • Active transport

Passive Transport

  • Diffusion: Movement of solutes from high to low concentration (down a concentration gradient).
  • Osmosis: Diffusion of water to assist in equilibration; water moves from a less concentrated solution to a more concentrated solution.
  • Facilitated Diffusion

Active Transport

  • Uses energy to move molecules against a concentration gradient (from low to high).
  • Example: Sodium-potassium pump, which exchanges one molecule for another (sodium out and potassium in).
  • Secondary Active Transport

Vesicular Transport

  • Transport via formation or release of vesicles.
    • Endocytosis
      • Phagocytosis (“cell-eating”)
      • Pinocytosis (“cell-drinking”)
      • Receptor-mediated endocytosis
    • Exocytosis

Cytoplasm

  • Cytoplasm = Cytosol and Organelles
  • Cytosol = solution comprised of suspended and dissolved molecules
  • Cytoskeleton = holds organelles and nucleus in place and supports the cell

Nucleus

  • Components:
    • Nucleoplasm
    • Nuclear envelope
    • Nuclear pores
    • Chromosomes
      • Deoxyribonucleic acid (DNA) – structural and functional characteristics
    • Ribonucleic Acid (RNA)
    • Nucleolus
    • Ribosomes – site of protein synthesis.
      • Produce proteins used inside the cell and proteins secreted from the cell.

Endoplasmic Reticulum

  • Continuation of the nuclear envelope.
    • Rough Endoplasmic Reticulum: Has ribosomes attached.
    • Smooth Endoplasmic Reticulum: Absence of ribosomes.
      • Produces lipids (cholesterol, steroid hormones, phospholipids, carbohydrates).
      • Involved in detoxification.

Golgi Apparatus

  • Membranous sacs that modify, package, and distribute molecules produced by the endoplasmic reticulum.

Mitochondria

  • Production of ATP (cell’s energy plant).
  • Involved in the Citric Acid cycle and Electron Transport chain.
  • Aerobic.
  • Capacity to increase in number within a cell.

Digestive System Functions

  1. Ingestion
  2. Mastication – food is mechanically broken down to increase surface area for digestive enzyme access
  3. Propulsion – the movement of food through the digestive tract
    • Swallowing (Deglutition) – moves the bolus (mass of liquid and soft food) from the oral cavity to the oesophagus
    • Peristalsis
      • Wave of smooth muscle relaxation which expands the tract in front of the bolus
      • Wave of smooth muscle contraction behind the bolus which propels the bolus forward (Oesophagus and Small Intestine)
    • Mass Movements
      • Contractions that move material (Large Intestine)
  4. Mixing
    • Segmental contractions
      • Segments fluctuate between relaxation and contraction
      • Purpose is to mix the food with digestive secretions which assist with breaking it down into smaller pieces (Small Intestine)
  5. Secretion
    • Purpose is to = digest, buffer, lubricate and liquefy
    • Types of secretions:
      • Mucus – entire digestive tract
        • Protects the epithelial cells from digestive enzymes, stomach acids and abrasion
        • Lubricates the lining of the tract and the food
      • Water
        • Liquefies the food – easier to digest and absorb
      • Enzymes – secreted from pancreas, small intestine, stomach and oral cavity
        • Breaks down complex food molecules to smaller molecules
  6. Digestion
    • Mechanical – mastication and mixing
    • Chemical – digestive enzymes
  7. Absorption
    • Molecules moving out of the digestive tract into the circulatory or lymphatic system
  8. Elimination
    • Waste products are eliminated from the body via defecation
    • Occurs in the large intestine, material is altered from a liquefied substance to semi-solid (feces) substance by absorbing water and

Chemical Digestion

  • Food we ingest needs to be broken down to smaller molecules
  • This is occurs via chemical reactions
  • These can occur naturally, but are quite slow
  • Require a Catalyst
  • Enzyme: a substance that increases the speed of a reaction without being changed or used up in the process
  • An enzyme is a type of catalyst (biological catalyst)
  • Protein

Digestive System Anatomy

  • Digestive Tract (alimentary tract or canal)
    • Tube from the mouth to anus
  • Accessory organs
    • Glands that secrete fluid into the digestive system
  • NOTE:
    • Gastrointestinal (GI) tract is only the stomach and intestines
  • Digestive system components in order:
    • Oral cavity (mouth)
      • Accessory organs = Tonsils and Salivary glands
    • Pharynx (throat)
    • Oesophagus
    • Stomach
    • Small intestine
      • Duodenum, Jejunum and Ileum
      • Accessory organs
        • Liver, Gallbladder and Pancreas
    • Large intestine
      • Cecum, Colon, Rectum and Anal canal

Layers of the Digestive Tract

  • 4 Layers (tunics)
  1. Mucosa
    • Mechanoreceptors
    • Chemoreceptors
  2. Submucosa
    • Nerves, lymphatic and blood vessels and small glands
  3. Muscularis
    • Smooth muscle
  4. Serosa
    • Smooth layer that covers the digestive tract organs

Regulation of the Digestive System

  • Nervous regulation
    • Enteric nervous system (ENS)
      • A division of the ANS
      • Located within the walls of the digestive tract
      • Can work independently of the ANS
    • Autonomic Nervous System
      • Parasympathetic NS
        • Rest and Digest
        • Increases motility, salivary and gastric acid secretions
      • Sympathetic NS
        • Fight and Flight
        • Decreases motility
  • Chemical regulation
    • Endocrine secretions (hormones):
      • gastrin, secretin and cholecystokinin

Oral Cavity

  • Mechanical and Chemical digestion
  • Normal adult teeth number = 32
  • Assists with the process of mastication – mechanical digestion
    • Incisors and canines tear and cut food
    • Premolars and molars grind and crush food

Saliva

  • 3 large salivary glands
  • 1-1.5 L / day
  • Watery/mucous secretion
  • Functions of Saliva
    1. Keeps the oral cavity moist
      • Assists with normal speech
    2. Suspension of food in a solution which aids taste
    3. Begins digestion
      • Salivary Amylase
        • starch splitting enzyme which breaks down 3-5% of carbohydrates
      • Lingual Lipase
        • digests small amounts of
    4. Protectivefunctions;
      • Washes oral cavity
      • Contains bicarbonate ions = neutralize acids from oral bacteria
      • Contains the enzyme Lysozyme
        • antibacterial properties which assists with preventing infection from bacteria
  • Parasympathetic Stimulation
    • In response to taste, tactile stimuli and food odor

Pharynx

  • Three parts:
    • Nasopharynx
    • Orophraynx
    • Laryngopharynx
      • Extends to the esophagus
  • Only the Oropharynx and Laryngopharynx are associated with digestion
  • Uvula/soft plate prevents food material entering the Nasopharynx
  • Epiglottis - covers the Larynx to prevent food entering the respiratory passages

Oesophagus

  • From pharynx to stomach
  • Posterior to the trachea
  • Travels through the opening (hiatus) of the diaphragm
  • Food into and out of the oesophagus is controlled by the upper and lower oesophageal
    • Upper is skeletal muscle; lower is smooth muscle

Phases of Swallowing

  1. VoluntaryPhase
    • The bolus is moved by the tongue against the hard palate and towards the oropharynx
  2. PharyngealPhase
    • The soft palate elevates to close of the nasopharynx
    • The pharynx elevates, receives the bolus and moves it towards the oesophagus
    • The epiglottis closes over the larynx
    • Upper oesophageal sphincter relaxes
  3. OesophagealPhase
    • Peristaltic waves move food towards the stomach
    • Gravity is enough for liquid
    • Lower oesophageal sphincter relaxes

Stomach Anatomy

  • Pyloric sphincter
    • regulates the release of food from the stomach into the small intestine

Stomach Histology

  • Submucosa and mucosa are folded (rugae), which assists with increasing its surface a the stomach receives food
  • The most inner layer forms gastric pits which are openings of the gastric glands
  • 5 types of epithelial cells make the gastric glands
    • Mucous
      • Protects the stomach lining from digestive enzymes and acids
    • Hydrochloric acid
      • Produces low pH in the stomach (pH = 1-3)
      • Primary role is to kill bacteria
      • Inactivates salivary amylase
      • Assists with the functioning and activation of pepsin
    • Enzymes
      • Gastric Lipase
        • Breaks down fats/lipids
      • Pepsinogen
        • converted to pepsin once in the stomach and comes into contact with hydrochloric acid and previously formed peps
      • Pepsin
        • Breaks down protein
    • Hormones
      • Gastrin
        • Increases gastric secretions

Regulation of Stomach Secretions

  1. CephalicPhase = “get started” phase
    • Stimuli=
      • Smell and taste of food
      • Chewing and swallowing
      • Pleasant thoughts about food
    • Parasympathetic action potentials cause:
      • Secretion of stomach mucosa
      • Increases chief and parietal cell secretions
      • Stimulates secretions of Gastrin
  2. GastricPhase = “go for it” phase
    • Stimuli=
      • Enlargement of the stomach (smoothing out of the rugae) as food enters
      • Activation of mechanoreceptors (stretch receptors)
      • Presence of protein
    • Leads to an increase in secretions in a similar fashion to the cephalic phase
    • Distension also causes a local increase in secretions
  3. IntestinalPhase = “slow down” phase
    • Low pH chyme entering the duodenum simulates chemoreceptors in the duodenum
    • Chyme containing lipids and Fatty acids and digested protein products entering the duodenum = inhibits gastric secretions
    • Increases secretion of secretin and cholecystokinin
    • Enterogastric reflex =
      • distension of duodenal wall, irritating substances, reduced pH = reduced gastric secretions

Small Intestine Anatomy

  • ~ 6 m long
  • Accessory organs
    • Liver, Pancreas and Gallbladder
  • Function
    • digestion and absorption
    • Site of the greatest amount of absorption
  • 3 parts
    1. Duodenum (25 cm)
      • Starts at the pyloric sphincter and ends at the jejunum
      • Entry point of ducts from the Liver and Pancreas
      • The surface area and thus absorption capability is greatly increased by the presence of circular folds, villi and microvilli
    2. Jejunum (2.5 m)
      • Similar in structure to the duodenum
      • Becoming smaller
      • Major site of absorption
    3. Ileum (3.5 m)
      • Smaller in;
        • Diameter
        • Wall thickness
        • Number of folds and microvilli
      • Less absorption
      • Ileocacal sphincter and valve
        • one way valve flow to the large intestine

Small Intestine Secretions

  • Mucus, electrolytes and water
    • Lubricates and protects the wall from acidic chyme
    • Keeps the chyme in liquid form to assist with continued digestion
  • Hormones
    • Cholecystokinin and Secretin
      • Decreases gastric secretions
      • Stimulates liver and pancreas secretions
      • Pancreas and liver secretions enter into the duodenum and assist with digestion
  • Enzymes
    • Disaccharidases and Peptidases
      • Assist with the breakdown of food
  • Segmental and peristaltic contractions mix and move chyme in the small intestine

Liver

  • Largest gland in the body
  • Second largest organ
  • Situated in the upper right quadrant of the abdomen
  • Has two lobes a right and left separated by a ligament
  • The porta on the inferior surface is an opening (gate) which allows the following to enter and ex Liver
  • Hepatic Artery
    • Carries oxygenated blood from the heart to the liver
  • Hepatic portal vein
    • Carries nutrient rich, deoxygenated blood from the small intestine to the liver
  • Carries bile from the liver to the Gallbladder and Duodenum

Bile

  • Bile is produced by the Hepatocytes
  • From the hepatic cords bile is collected by the bile canaliculus
  • Flow towards the hepatic triad and exits via the hepatic ducts

Liver Functions

  1. BileProduction:
    • Bile enters duodenum and neutralizes and dilutes stomach acid as a low pH renders the effectiveness of pancreatic enzymes
    • Contains bile salts which emulsifies lipids
      • Lipids are hydrophobic
      • Therefore, they clump together
      • Bile helps break that clump apart
      • Enzymes can then breakdown lipids
      • Bile Contains NO digestive enzymes
    • Stimuli
      • parasympathetic stimulations, secretin secretion, bile salts (positive feedback)
      • Secretion continues until the duodenum e
  2. Storage
    • Sugar (as glycogen), lipids, vitamins
    • Storage fluctuates depending on the bodies needs
  3. Inter-conversion of Nutrients
    • The correct nutrients are not always present in the required amounts thus the liver can convert nutrients to others if need be
  4. Detoxification
    • By-products produced during metabolism and ingested toxins are further broken down thus altered to less harmful toxins in the liv
      • Alcohol – Liver is the primary organ which breaks down alcohol
  5. Synthesis of Molecules
    • Plasma proteins and clotting factors

Gallbladder

  • On the inferior surface of the liver
  • Three layers;
    • Inner mucosa folded into rugae which expand
    • Muscularis layer of smooth muscle which contracts and assists with expelling bile
    • Outer serousa
  • Acts as a storage site for Bile secreted from the liver
    • Water and electrolytes are absorbed = concentrated substance
  • Stimuli
    • secreted in response to cholecystokinin

Pancreas

  • Exocrine role
    • secretes substances that exit onto a surface or through a duct
  • Pancreatic Juice
    • Aqueous component
      • Most important part of the aqueous component is the bicarbonate ions
        • Neutralizes stomach Chyme
        • Stops pepsinogen working
        • Creates a suitable environment for pancreatic juice to function
    • Enzyme component
      • Proteolytic pancreatic enzymes – digest proteins
        • Secreted in their inactive form to prevent digestion of the pancreas where they are produced
          • Trypsin
            • activates the secretion of other proteolytic enzymes
      • Chymotrypsin
      • Carboxypeptidase
      • Pancreatic amylase
        • Continues the work of salivary amylase - the break down of carbohydrates
      • Pancreatic lipase
        • Breaks down lipids

Regulation of Pancreatic Secretions

  • Chyme entering the duodenum causes release of:
    • Secretin
      • which stimulates secretion of the aqueous component
    • Cholecystokinin
      • stimulates the enzyme component of pa
  • Parasympathetic stimulation causes the release of pancreatic juice particularly during the ceph
  • Hepatic ducts (left and right) exit liver e common hepatic duct and cystic duct (which exits the common bile ducte hepatopancreatic ampulla (common bile duct + pancreatic duct meet)e con duodenum (small intestine) through the major duodenal papilla

Summary of Digestive Hormones

  • Gastrin
    • Stimulated by distension of stomach and the bolus entering the stomach
    • Increases gastric secretions
  • Secretin
    • Stimulated by chyme entering the duodenum
    • Decreases gastric secretions
    • Stimulates pancreas to secrete pancreatic juice
    • Stimulates bile secretion from liver
  • Cholecystokinin
    • S ente
    • D secre
    • S secre
    • S from

Digestive Enzymes

  • Amylase
    • Break down Carbohydrates
  • Lipase
    • Break down Lipids
  • Protease
    • Break down Proteins
  • What affects enzyme function?
    • Temperature
    • pH
  • Typically enzyme names end in (suffix is) “ase”

Cardiovascular System Functions

  • Blood
    • From the heart to the tissues and back to the heart again
    • Exchange nutrients, waste products and gases with tissues
      • Nutrients and Oxygen move from the vessels to the tissues
      • Waste products and Carbon dioxide move from the tissue to the vessel
    • Transport substances
      • Hormones, enzymes, nutrients, gasses, waste products are all transported in blood
    • Assists with regulation of blood pressure
      • Heart and blood vessels work together
    • Directs blood flow to tissue
      • In order to maintain homeostasis the circulatory system can direct to tissues in need

The Circulations

  • Systemic Circulation:
    • Heart (Left ventricle) a body tissues
  • Pulmonary Circulation:
    • Heart (Right Ventricle) a lungs (alveolus)
  • Coronary Circulation:
    • Myocardium

Cardiovascular Components

  • Heart
  • Blood Vessels
    • Arteries
    • Capillaries
    • Veins

Blood

  • Functions as a dynamic tissue:
    • Transport: of substances from one area of the body to another
    • Immunity: body’s defence against disease
    • Haemostasis: processes to stop bleeding
    • Homeostasis: process of maintaining a stable internal environment
      • Transport of regulatory molecules
      • Regulation of pH
      • Maintenance of body temperature
    • Immunity
  • Total blood volume 4-6 L (depending on body size)

Components of Blood

  • Plasma
    • Composed of water with some dissolved solutes including proteins, lipids (fats), carbohydrates, amino acids, vitamins, minerals hormones, wastes, gases and electrolytes
  • Platelets (Thrombocytes)
    • Cell-like particles, assist in blood clotting
  • White Blood Cells (Leukocytes)
    • Primary defence against infection
  • Red Blood Cells (Erythrocytes)
    • Main component is haemoglobin

Haemoglobin

  • Protein substance with 4 subunits:
    • 4 x Globins
    • 4 x Heme groups
  • Transport of O2 and CO2
    • Oxygen binds to the heme group
      • 1 O2 molecule to 1 heme group
      • Thus 1 haemoglobin molecule carries 4 O2 molecules
      • 280 million haemoglobin molecules per red blood cell
    • Haemoglobin gives the red blood cell its colour
      • Oxyhemoglobin is bright red
      • Deoxyhemoglobin is darker red
    • Carbon dioxide binds to the globin portion
      • 23% of CO2 is transported by haemoglobin
      • 7% in plasma
      • 70% in the form of bicarb ions

Production of Red Blood Cells

  • 25 trillion RBC’s in circulation
  • Every second 2.5 million are destroyed
  • 1% replaced each day
  • Lifespan = 120 days
  • Production of new red blood cells = Erythropoiesis

Heart Anatomy

  • Superior and
  • Right
  • Tricuspid
  • Right
  • Pulmonary
  • Pulmonary
  • Pulmonary
  • Pulmonary
  • Left
  • Bicuspid
  • Left
  • Aortic
  • Inferior
  • Atrium
  • Valve
  • Ventricle
  • Semilunar
  • Artery
  • Capillaries
  • Vein
  • Atria
  • valve
  • Ventricle
  • Semilunar
  • Aorta
  • Vena
  • valve
  • Valve
  • Cava

Two Pumps

  • The heart functions as two separate pumps:
    • One pump (right side) receives blood from the body and pumps it to the lungs for oxygenation
      • Pulmonary Circulation
    • The other pump (left side) receives blood from lungs and circulates it around the body and back to the heart
      • Systemic Circulation

Cardiac Muscle Structure

  • Striated, branching chains of cells that uni- or binucleate
  • Fibres connected mechanically and electrically
  • Functional syncytium
  • Adjacent cardiac muscle cells are joined end to end by intercalated discs, which contain two types of specialized junctions:
    • Desmosomes
      • Mechanically holding the cells together
    • Gap junctions
      • Permit action potentials to propagate from cell to cell

Cardiac Function

  • Cardiac Cycle
    • All mechanical and electrical events that occur during one heartbeat
    • Diastole - Relaxation phase
      • Chambers fill with blood
      • Twice as long as systole
      • Ventricle untwists, lengthens, unthickens
    • Systole - Contraction phase
      • Chambers eject blood
      • High pressure
      • Ventricle twists, shortens, thickens
      • “wet towel”

Regulation of Heart Rate

  • Pacemaker (SA node)
    • Intrinsic firing rate ~100bpm
  • Influenced by Autonomic Nervous System (ANS)
    • Sympathetic and parasympathetic branches
      • At rest, parasympathetic stimulation decreases HR (~60-70 bpm)
      • During exercise, withdrawal of vagal tone, concurrent increase in sympathetic nerve innervation to increase HR
    • Hormonal Influence
      • Sympathetic components increase HR by release of catecholamines: adrenaline and noradrenaline
  • Electrocardiogram

Electrocardiogram (ECG)

  • Recording of hearts electrical activity
  • Diagnostic tool
  • 12-Lead ECG
    • Different electrical ‘view’s of the heart

Electrocardiogram (ECG)

  • P wave
    • Impulse arising from the SA node results in depolarization and contraction of the atria (the right atrium contracts slightly before the left atrium).
    • This atrial depolarization = atrial systole.
  • QRS Complex
    • Due to ventricular depolarization, marks the beginning of ventricular systole.
    • Masks the underlying atrial repolarization signal.
  • T wave
    • Ventricular repolarization
    • End of T wave marks end of ventricular systole electrically
  • P wave:
    • Atrial depolarization (atrial contraction)
  • QRS complex:
    • Ventricular depolarization (ventricular systole commences)
  • T wave:
    • Ventricular repolarization

Capillaries

  • Microcirculation
    • One endothelium cell layer thick walls for rapid transfer of substances
    • Link between the arterial and venous systems
    • Blood flow regulated by precapillary sphincters
    • Important function in body temperature regulation
      • e.g. skin

The Venous System

  • One-way valve system to prevent back flow
  • Skeletal muscle pump assists against gravity
  • ‘Capacitance’ vessels

Pulmonary Circulation

  • Is the portion of the Cardiovascular system that oxygenates the blood and removes CO2
  • Pulmonary arteries carry de-oxygenated blood from the heart to the lungs
  • Pulmonary veins carry oxygenated blood from the lungs back to the heart

Cardiovascular Terminology

  • Heart Rate (HR)
    • The number of times the heart beats per minute
  • Stroke volume (SV)
    • Volume of blood pumped out of the heart with each beat
  • Cardiac Output
    • Total volume of blood pumped out of the heart per minute
    • CO=HR×SVCO = HR \times SV
  • Ejection fraction
    • The fraction of blood pumped out of the left ventricle with each contraction
  • Systolic blood pressure (~120 mm Hg)
    • Maximal pressure of blood against the arterial's walls during systole (heart contraction; ventricle contraction)
    • Estimate of how hard the heart is working and strain on arterial walls during ventricular contraction
  • Diastolic blood pressure (~70-80 mm Hg)
    • Pressure exerted by blood against the arterial walls during diastole (heart relaxation; ventricle relaxation)
    • During relaxation (diastolic) phase of cardiac cycle, arterial blood pressure decreases
    • Indicate peripheral resistance i.e. the ease with which blood flows from arterioles into capillaries

Muscular System Functions

  • The primary function:
    • produce movement in the body
  • Other functions:
    • Constriction of organs and vessels
    • Cardiac contraction
    • Respiration
    • Postural maintenance
    • Body heat production

Types of Muscle

  • Muscle
  • Muscle
  • Muscle

Muscle Bundles

  • Bundles of Muscle Fibers = Fascicle (singular), fascicles (plural)
  • Muscle Fibre
    • A single muscle cell
  • Myofibrils
    • Singular
      • Myofibril
  • Myofilaments
    • Singular
      • Myofilament

Myofibril

  • Sarcomere
    • Contractile unit of muscle
    • Z-line to Z-line
    • Striation of myofibrils reflects overlapping of thick and thin filaments
    • M-line:
      • Myosin attachment
    • Titin enables the muscle to stretch and recoil

Skeletal Muscle Organelles

  • Sarcolemma (Plasmalemma)
    • Cell membrane is composed of the sarcolemma and basement membrane
    • Fuses with tendon which connects muscle to bone
    • Assists with the transmission of action potentials along the muscle
    • Transports metabolites in and out of cell
  • Sarcoplasmic Reticulum
    • Specialised smooth endoplasmic reticulum in skeletal muscle, network of tubules run along and around myofibrils
    • Storage site of calcium
  • Transverse Tubules (T-tubules)
    • Extensions of the sarcolemma that pass laterally through the cell
    • Allow action potentials to be transmitted into the myofibrils
  • Other organelles:
    • Sarcoplasm
    • Mitochondria
    • Nucleus
    • Multi-nucleated

Muscle Fibre Types

  • Skeletal muscle is not made up of homogenous fibre that share metabolic and contractile properties
  • Several types differing in mechanism of ATP production, motor neuron innervation, force production capacity

Muscle Fibre Distribution

  • The predominance of muscle fibre types are determined by:
    • Function of the muscle
      • Postural
        • Slow-Twitch
      • Rapid movement of aerobic nature
        • Fast twitch
      • Rapid, powerful movement
        • Fast twitch
    • Genetics
    • Training…?

Sliding Filament Model/Theory

  • Interaction of myofilaments cause muscle contraction/shortening
  • Myofilaments slide past one another = the sarcomere shortens = the myofibril shortens
  • When a sarcomere shortens, myofibril, muscle fibre, muscle fascicles and entire muscle shorten to produce muscular contraction

Myosin

  • Thick filaments are bundles of Myosin molecules
  • Each globular ‘head’ of the myosin has:
    • Myosin ATPase site
    • Actin binding site

Actin

  • Thin filaments are paired chains of actin molecules each with a myosin binding site

Sliding Filament Theory At rest

  • At rest the binding sites are covered by the regulatory proteins Troponin and Tropomyosin
  • Role of Calcium (Ca2+)
    • At rest, myosin and actin are unable to bind due to Troponin and Tropomyosin
      • Tropomyosin covers the binding sites on actin
      • Troponin holds Tropomyosin in place
      • Troponin is the ‘lock’ that keeps binding sites inaccessible
      • Calcium is the ‘key’

Role of Calcium (Ca2+)

  • Ca2+ is stored in the Sarcoplasmic Reticulum
  • Upon release it spreads out in the sarcoplasm and binds to Troponin
  • Troponin initiates the contraction process by moving Tropomyosin away to expose the Myosin binding sites on actin
  • Ca2+ binds to Troponin
    • Changes shape, moves Tropomyosin aside and exposes the myosin binding site
    • Cross bridging occurs
      • attachment of Myosin to Actin

Energy

  • Adenosine Triphosphate = ATP

Stages of Sliding Filament Theory

  1. Exposure of Active Sites
  2. Cross-Bridge Formation
  3. Power Stroke