CH1: Microscopy
COMPOUND LIGHT
Consist of ocular lenses (binocular eye piece) and objective lenses
STEREOSCOPE
Dissecting scopes
Dalawa 'yung silipan
ELECTRON
Uses beams of electron which make it capable for higher magnification
SEM: Scanning
| TEM: transmission
|
MICROGRAPH
Images produced by microscope
VARIABLES USED IN MICROSCOPY
MAGNIFICATION
Amount or degree of visual enlargement of an observed object
OBJECTIVES
Color | Objective | Magnification | Total magnification |
Red | Scanner | 4x | 40x |
Yellow | LPO | 10x | 100x |
Blue | HPO | 40x 45x | 400x 450x |
White | OIO | 100x | 1000x |
TOTAL MAGNIFICATION
M of eyepiece x M of objectives
Ex. 10x x 4x = 40x Total magnification
MICROMETRY
Estimation
FIELD OF VIEW/FOV
Formula:
CH1: Microscopy
COMPOUND LIGHT
Consist of ocular lenses (binocular eye piece) and objective lenses
STEREOSCOPE
Dissecting scopes
Dalawa 'yung silipan
ELECTRON
Uses beams of electron which make it capable for higher magnification
SEM: Scanning
| TEM: transmission
|
MICROGRAPH
Images produced by microscope
VARIABLES USED IN MICROSCOPY
MAGNIFICATION
Amount or degree of visual enlargement of an observed object
OBJECTIVES
Color | Objective | Magnification | Total magnification |
Red | Scanner | 4x | 40x |
Yellow | LPO | 10x | 100x |
Blue | HPO | 40x 45x | 400x 450x |
White | OIO | 100x | 1000x |
TOTAL MAGNIFICATION
M of eyepiece x M of objectives
Ex. 10x x 4x = 40x Total magnification
MICROMETRY
Estimation
FIELD OF VIEW/FOV
Formula:
