Human Growth & Development - Extra-embryonic Tissues

Learning Outcomes

  • Understand the difference between embryonic and extraembryonic tissues
  • Describe the formation of the extraembryonic membranes

Embryonic vs Extraembryonic Tissues

  • Embryonic Tissues: Tissues contributing to structures retained in the fetus (i.e., become part of the born individual).
  • Extraembryonic Tissues: Tissues primarily giving rise to support structures for the embryo during its development (i.e., not retained in the individual after birth).

Types of Extraembryonic Membranes

  • Amnion: Surrounds the embryo, creating the amniotic cavity.
  • Yolk Sac: Provides nutrients and supports early blood cell formation.
  • Chorion: Involved in gas exchange and nutrient transfer.
  • Allantois: Vestigial in humans; stores nitrogenous wastes in birds/reptiles.
Importance of Extraembryonic Membranes
  • Enable vertebrates to reproduce on land, independent of aquatic environments.
  • Ensure nutrient supply and gas exchange to developing embryo.
  • Prevent dehydration of embryo.

Key Phases of Embryonic Development (Week 1)

  1. Fertilization: Sperm and egg fusion forms a zygote.
  2. Cleavage: Zygote undergoes multiple cell divisions forming a blastocyst.
  3. Blastocyst Formation: Comprises an outer trophoblast layer and an inner cell mass.
  4. Implantation: Blastocyst attaches to uterine lining.

Details: Day 8 Development

  • Blastocyst Embedding: Partially embedded into endometrium.
  • Differentiation of Trophoblast:
    • Cytotrophoblast: Inner layer, mononucleated.
    • Syncytiotrophoblast: Outer layer, multinucleated, erodes maternal tissue leading to formation of lacunae filled with maternal blood.

Day 8: Bilaminar Disc Formation

  • Inner Cell Mass Differentiates:
    • Hypoblast: Cuboidal cells facing blastocyst cavity.
    • Epiblast: Columnar cells facing the amniotic cavity.
  • Amniotic Cavity: Cavity within epiblast, lined by epiblast and amnioblasts forming the amnion.

Day 9: Yolk Sac Formation

  • Blastocyst Embedment: Trophoblast reaches lacunar stage.
  • Development: Vacuoles in Syncytiotrophoblast fuse to form lacunae.
  • Exocelomatic Membrane Formation: Formed from flattened hypoblast cells, lining the exocelomatic cavity/primitive yolk sac.

Day 11/12: Uteroplacental Circulation

  • Complete Embedding: Blastocyst is fully embedded in endometrial stroma.
  • Syncytiotrophoblast Invasion: Penetrates deeper, eroding maternal capillaries, allowing maternal blood to flow into trophoblastic system.

Day 11/12: Extraembryonic Mesoderm Formation

  • Cells appear between cytotrophoblast and outer yolk sac surface, forming extraembryonic mesoderm as loose connective tissue, filling the space externally and internally.

Day 11/12: Formation of Extraembryonic Coelom

  • Cavity Development: Large cavities in extraembryonic mesoderm merge to form the extraembryonic coelom/chorionic cavity, surrounding yolk sac and amniotic cavity, except where connected by the body stalk.
  • Structures:
    • Somatopleuric Mesoderm: Lining cytotrophoblast and amniotic cavity.
    • Splanchnopleuric Mesoderm: Lining covering yolk sac.

Day 13: Primary Villi Formation

  • Proliferation of Cytotrophoblast: Cells proliferate and infiltrate Syncytiotrophoblasts to form primary villi.

Day 13: Secondary Yolk Sac Formation

  • Additional Hypoblast Cell Migration: Forms a new cavity (secondary yolk sac) inside the exocoelomic cavity.
  • Importance: Vital for metabolising and transporting nutrients to the embryo, and as a site for primitive blood cell production (haematopoiesis).

Day 13: Chorionic Cavity & Plate

  • Expansion: Extraembryonic coelom enlarges to form chorionic cavity.
  • Chorionic Plate: Lined by extraembryonic mesoderm and forms the umbilical cord from the connecting stalk.

Allantois Functionality

  • Function in Humans: Vestigial structure with limited function.
  • Function in Other Species: Serves as a storage organ for nitrogenous wastes in birds and reptiles.

Summary of Week 2 Development

  • Trophoblast Differentiation:
    • Into Cytotrophoblasts and Syncytiotrophoblasts.
  • Inner Cell Mass Differentiation:
    • Into Epiblast and Hypoblast.
  • Cavities Formation:
    • Amniotic and yolk sac cavities.
  • Mesoderm Division:
    • Into somatopleuric and splanchnopleuric primary mesoderm.