Vision (Lecture)
- The eye has 6 muscles:
- 4 rectus: superior, inferior, medial, lateral
- 2 oblique: superior and inferior
- The optic nerve is millions of axons merged together
- Fovea Centralis: The sharpest vision due to high density of cones
- Optic disk is the blind spot, where the optic nerve exits
- Refraction is the bending of light rays as they pass through the eye
- The anterior chamber is BEFORE the lens
- The optic chiasm is a SPACE not a structure, where fibers from each eye go to the left and right side of the brain, basically where fibers cross
- In the blue/green picture, it is where the optic tract from the nasal side of the eye cross to go to the other hemisphere, which the “safety mechanism”
- After the optic chiasm are the optic tracts that lead to the thalamus (neurons of the thalamus form the projection fibers/optic radiation)
- The retina has two layers:
- Pigmented
- Associated with the choroid, separates sensory cells
- Reduces light scattering, absorbs extra light rays
- Neural
- Has 3 types of cells/neurons
- Photoreceptors
- Photoreceptors → Bipolar Cells → Ganglionic
- Rods (dark and light; black and white)
- Type of bipolar photoreceptor, not found in fovea centralis, but found almost all over the retina
- More sensitive to light
- Contains rhodopsin (found in disks)
- Rhodopsin can be broken into opsin and retinal (contains vitamin A) when light hits
- They combine in the absence of light
- Rods are DEPOLARIZED at rest, and when light hits, they become HYPERPOLARIZED
- Na+ is ALWAYS flowing into the outer segment of the rod
- Cones (color)
- Have iodopsin (red, blue, green pigments) instead of rhodopsin
- Mostly in the fovea centralis, where the highest visual acuity is (due to cones)
- When light density goes down, there is less sight of colors
- EXTRA INFO ON RODS AND CONES
- BOTH RODS AND CONES HAVE OUTER AND INNER SEGMENTS AND DISKS
- THE INNER SEGMENTS HAVE ORGANELLES, THIS PART PUMPS OUT NA+
- THE OUTER SEGMENT TAKES IN NA+
- BOTH ALSO HAVE THE DARK CURRENT CYCLE
- CONES HAVE IODOPSIN; RODS HAVE RHODOPSIN
- Disks contain rhodopsin, and when rhodopsin runs out, the top disk gets phagocytized, and the disks get replenished
- Light and dark adaptation: the ability of the eyes to adjust to light and dark situations by the movement of the pupil, the availability of rhodopsin, and the changes in photoreceptor function
- When rods/cones are depolarized, it causes bipolar and ganglionic cells to be depolarized too
- The neurotransmitter glutamate (released by photoreceptors) get picked up by bipolar cells
- Glutamate decreases with light absorption
- Bipolar cells release a neurotransmitter that gets picked up by ganglionic cells
- Then the ganglionic cell produces an action potential that goes to the optic disk
- Rhodopsin (opsin big, retinol small) is embedded into the phospholipid bilayer of the disk, and G protein (transducin, and alpha, beta, and gamma) is beside the rhodopsin
- The sodium gated channels are open during rest (allowing the dark current) because of cGMP
- There are two halves to each eye, the nasal and temporal sided
- This is basically a safety mechanism because the two halves go to different sides of the brain, so if you have a stroke, you won’t completely lose vision
- ]]RHODOPSIN CYCLE (when light hits, it created a cascade effect):]]
- (In the outer segment disk) Retinol changes shape (bent [11-cis-shape] → linear [all-trans-shape])
- Opsin changes shape because of that
- That activates transducin (G protein, 3 subunits)
- Which activates the enzyme cyclic GMP phosphodiesterase (THE ENZYME)
- Catalyzes cGMP to GMP (NEITHER ARE THE SAME AS ENZYME)
- The sodium channels close and the dark current stops
- Rod cells are now hyperpolarized
- Retinol and opsin separate
- The G protein reunites (3 subunits)
- The enzyme deactivates, the channels are closed
- In the absence of light, the retinol goes back to a bent shape
- Then it binds to opsin
- cGMP returns to the base of the sodium gated channel, opening it, causing it to depolarize again
- The dark current returns