DNA Analysis and Extraction Notes

Restriction Enzymes

• The purpose of restriction enzymes is to create DNA fragments of varying lengths. These lengths differ from person to person due to variations in individual DNA codes.

Electrophoresis

• DNA fragments move when power is applied due to electrophoresis.

• Electrophoresis separates DNA fragments, distributing them in a gel based on their lengths.

DNA Matching

• Based on the provided DNA fingerprint, Honey's DNA matches that of the suspect.

DNA Extraction Purposes

• DNA extraction purifies DNA away from proteins and other cellular contaminants.

DNA Organization

• Organization of DNA from largest to smallest:

  • Cell

  • Nucleus

  • Chromosomes

  • Histone

  • DNA

Lysis Solution and Warm Water Bath

• Lysis solution combined with a warm water bath helps break open cells and release the DNA.

Purpose of Salt

• Salt is used to help stabilize the DNA.

Purpose of Centrifuge

• The purpose of the centrifuge is to separate the different components of a solution based on their densities.