heather trivia 1114

  1. 4 reagents of gram stain

  • Crystal violet

  • Grams Iodine

  • Ethanol - ethyl alcohol

  • Safranin



  1. Name 1 method of fixing a slide

Heat, or alcohol 


  1. What colour does a gram - bacteria appear in gram stain

  • (-) pink/red

  • (+) blue/purple


  1. 2 common used culture media

  • MAC - MacConkey 

  • BAP - Blood agar plate


  1. Name a common differential media

  • BAP - (for particular organism)

  • MacConkey - selective (gram -) & differential (lactose fermenting)


  1. What volume is a urine culture loop calibrated to

  • 0.001mL

  • 10uL


  1. t/f a smear should be made before a culture plate from a specimen swab

  • False, slide is not sterile so culture plate first


  1. What is a parasite?

  • An organism that lives within another living organism to its own benefit + at an advantage

  • An organism that lives upon or within another living organism


  1. Which is larger? Viruses or bacteria

  • Bacteria


  1. Bacterial sensitivity can only be done on what type of cultures?

  • Pure colony / culture, single colony

  1. What is another name for the anatomical pathology department

Histology, histopathology, surgical pathology


  1. What r 3 major steps in tissue processing

  1. Dehydration

  2. Clearing / dealcoholization

  3. Paraffin wax infiltration


  1. When would a frozen section be done

When urgent diagnosis is required, can be frozen instead of being embedded in paraffin

Ex. surgery, rapid section can be examined within 5 mins using an instrument called cryostat

  • Use liquid nitrogen to freeze specimen


  1. What fixative is used for cytology specimen

Alcohol; ethanol, methanol

Formalin & formaldehyde in histology


  1. t/f first step of processing a histology specimen is embedding

False 

  1. Fixing

  2. Grossing

  3. Tissue processing; dehydration, clearing, paraffin wax infiltration

  4. Embedding

  5. Cut

  6. Stain

  7. Mounting

  8. Viewed bt pathologist

  9. Filling specimen


  1. Ability of one solution to mix with another

Miscibility


  1. What 2 areas is cytology generally divided into

Gynecological (pap-smears)  & non-gynecological (not)


  1. What type of anticoagulant is acceptable for a type & screen

EDTA tube

  1. D-dimer tests r run by which sub-department

Hematology-coagulation studies


  1. The hemolysis of RBC may affect what chemistry analyte

Potassium, LDH; lactate dehydrogenase


Full name, department & tube type

  1. bHCG

Beta human chorionic gonadotropin 

PST/SST

routine chem or tumour marker lab


  1. K+

Potassium, routine chem, PST/SST


  1.  PTT

Partial thromboplastin time; coag, citrate


  1. RF

Rheumatoid factor; serology, SST/RED


  1. PCV

Packed cell volume; hematology, EDTA

What tests r included in the CBC; RBCc, WBCc, Pltc, Hb, Hct, Red cell indices (MCV, MCH, MCHC, RDW)


  1. TDM

Therapeutic drug monitoring,

chemistry subdepartment, 

PST/SST, red, green