Dr. Liu_Detailed Study Guide-(HPLC, GC-FID, GC-MS, & FAA)

These flashcards cover essential components, functions, and methods related to HPLC, GC-FID, GC-MS, and FAA techniques.

What are the main components of an HPLC system?

Solvent reservoir, degasser, pump, injector/autosampler, column compartment, detector (UV-Vis DAD), waste bottle.

What is the function of the FID detector in GC-FID?

It detects the presence of compounds by measuring the current produced by ions formed when a substance is burned in a flame.

What are key parameters to set in a GC-FID temperature program?

Initial temperature, ramp rate, final temperature and hold, split ratio setting, carrier gas flow.

What must be ensured during sample preparation for HPLC?

Filter through 0.45 μm membrane, dilute to appropriate concentration, match solvent to mobile phase.

How does area under a peak relate to concentration in HPLC analysis?

Area under peak is proportional to concentration.

What is the significance of TIC in GC-MS?

TIC stands for Total Ion Chromatogram and provides a visual representation of all detected ions over time.

What does LOD stand for and what does it represent?

LOD stands for Limit of Detection and represents the smallest signal that can be reliably distinguished from noise.

How is the concentration determined using the Beer’s Law equation?

A = εcl, where A is absorbance, ε is molar absorptivity, c is concentration, and l is the path length.

What does the x-intercept represent in a standard addition graph for FAA?

The x-intercept indicates the concentration of the unknown sample.

What is the primary method of analysis in GC-MS?

Mass spectrum matching to identify compounds.