Ch10 Pt1: DNA replication

Pt1: How are nucleotides incorporated into replicated DNA? What is DNA polymerase and how does it work?

define semiconservative model

define conservative model

define dispersive model

meselson stahl question, which of these models are correct?

define density gradient centrifugation

What types of nitrogen does DNA contain

-Density Gradient Centrifugation: Material is layered on CsCl (Cesium chloride) gradient and centrifuged: heavier material moves further
→kind of like electrophoresis

-DNA contains nitrogen: 15N is heavier than 14N
→Therefore DNA containing 15N is heavier and moves further through CsCl gradient.

Start → Replication cycle 1 → Replication cycle 2 → Replication cycle3

seeing dna composition and DNA bands with types of Nitrogren in DNA

what would banding patterns look like under alternative models?

conservative model and dispersive model’s

14N, Int, 15N

semiconservative replication in eukaryotes

what did taylor hughes use and what did they do

used root tips of Vicia faba.  Monitored process of replication with 3H-thymidine label