Stomata are surrounded by 2 guard cells that **regulate transpiration rates** and **loss of water** through evaporation
* Day: stomata open, allows CO2 in and O2 out
* guard cells have chlorophyll, when sun out produce sugars → water moves into the guard cells, they swell, opening the stomata
* Night (or plant losing water): stomata close
* no sun, guard cells don’t make sugars → guard cells lose water, go limp, closing the stomata
* Typically more guard cells in the lower epidermis since this layer is not directly exposed to the sun and is cooler