Anaerobes

Anaerobic gram-negative cocci \=\> \____ spp.

Veillonella

Anaerobic gram-positive cocci \=\> \____ spp.

Peptostreptococcus

Anaerobic GNBs \=\> \___ spp., \___ spp., \___ spp., and \___ spp.

Bacteroides; Fusobacterium; Porphyromonas; Prevotella

Anaerobic spore-forming GPBs \=\> \___ spp.

Clostridium

Anaerobic non-spore-forming GPBs \=\> possible \____ spp.

Lactobacillus

Good growth in the presence of 20% bile and esculin hydrolysis (BBE plate) \=\> \___ \___ group

Bacteroides fragilis

Brick-red fluorescence under UV light \=\> \___ or \___

Porphyromonas; Prevotella

T/F: Porphyromonas grows on KVLB.

F

T/F: Prevotella will grow on KVLB

T

Double zone of hemolysis on sheep BAP \=\> \___ \___

Clostridium perfringens

Antibiotics in KVLB \= \___ and \___

kanamycin; vancomycin

T/F: B. fragilis will grow on KVLB

T

T/F: B. fragilis, Prevotella, and Peptostreptococcus will grow on CDC agar.

T

T/F: B. fragilis, Prevotella, and Peptostreptococcus will grow on PEA and CNA.

T

T/F: Prevotella will grow on BBE.

F

T/F: Peptostreptococcus will grow on BBE.

F

All aerotolerance test plates should be incubated at \___ degrees C for up to \___ hrs.

35; 48

Both Clostridium and Bacillus are gram-\___

positive

Propionibacterium acnes is indole \___; Propionibacterium spp. is indole \___

positive; negative

Clostridium is catalase \___; Bacillus is catalase \___

negative; positive

Clostridium \____ is the only Clostridium that is urease positive.

sordellii

Clostridium spp (except sordellii) is urease \___

negative

Campylobacter: motile or non-motile?

motile

SPS \= \___ \___ \___ disk

sodium polyanethol sulfonate

SPS sensitive \=\> \___ \___

Peptostreptococcus anaerobius

Resistance to vancomycin, kanamycin, and colistin \=\> \___ \___

Bacteroides fragilis

Resistant to kanamycin and colistin, but susceptible to vancomycin \=\> \____

Porphyromonas

Resistant to vancomycin and kanamycin, and susceptible to colistin \=\> \____

Prevotella

Boxcar-shaped bacilli \= \___ \___

Clostridium perfringens

T/F: see spores w/ Clostridium perfringens.

F

Barnyard or horse stable odor \= \___ \___

C. diff

Chartreuse fluorescence under UV light \=\> \___ \___

C. diff

Ground-glass colony morphology \=\> \___

Fusobacterium

\___ \= thin w/ pointed ends.

fusiform

T/F: Veillonella grows on KVLB.

F

2 important anaerobes to ID: \___ \___ and \___ \___

Clostridium perfringens; B. fragilis

T/F: anaerobic flora is prevalent on mucosal surfaces of GI and genital tracts \=\> most infections are endogenous.

T

Ambient air \= __% O2 and __% CO2

21; 1

Capnophilic \= __-__% CO2

5; 10

Microaerophilic < __% O2

5

Anaerobic \= __% CO2 / __% H2 / __% N2

10; 10; 80

CDC blood agar: vitamin __, \___ extract, and \___

K1; yeast; hemin

KVLB supports the growth of \___ and \___

Bacteroides; Prevotella

Use \___ \___ as a back-up in case of jar failure.

thioglycollate broth

\___: test for lecithinase and lipase.

EYA

Lecithinase and lipase production on EYA \=\> \___ or \___

Clostridium; Fusobacterium

T/F: S. aureus will grow anaerobically on CDC.

T

T/F: S. aureus will grow anaerobically on PEA.

T

T/F: S. aureus will grow on BBE

F

T/F: Strep/Enterococcus will grow anaerobically on CDC

T

T/F: Strep/Enterococcus will grow anaerobically on PEA

T

T/F: Strep/Enterococcus will grow on BBE

F

T/F: E. coli will grow anaerobically on CDC.

T

T/F: E. coli will grow anaerobically on PEA

F

T/F: E. coli will grow on BBE

F

T/F: B. fragilis will grow on anaerobic CDC.

T

T/F: B. fragilis will grow anaerobically on PEA or CNA.

T

T/F: B. fragilis will grow aerobically.

F

T/F: Cl. perfringens will grow aerobically.

F

T/F: Cl. perfringens will grow anaerobically on CDC.

T

T/F: Cl. perfringens will grow anaerobically on PEA or CNA

T

T/F: Peptostreptococcus will grow aerobically.

F

T/F: Peptostreptococcus will grow anaerobically on CDC

T

T/F: Peptostreptococcus will grow anaerobically on PEA or CNA

T

T/F: Veillonella will grow aerobically.

F

T/F: Veillonella will grow anaerobically on CDC.

T

T/F: Veillonella will grow anaerobically on PEA or CNA

T

T/F: Lactobacillus will grow aerobically

T

T/F: Lactobacillus will grow anaerobically

T

Which colony is bigger: Lactobacillus grown aerobically or anaerobically

anaerobically

T/F: Lactobacillus grows on MAC

F

T/F: Lactobacilllus grows on CDC

T

T/F: Lactobacillus grows on PEA and CNA

T

B. fragilis grows as \___ colonies on BBE

black

Clostridium \___, \___, and \___ are aerotolerant GPBs.

tertium; histolyticum; canis

Which are larger: colonies of Cl. tertium grown aerobically or anaerobically?

anaerobically

Staph \____ is an anaerobic Staph

saccharolyticus

BBE: \___% bile

20

Most common Bacteroides \= \___

fragilis

Second most common Bacteroides \= \____

thetaiotamicron

Most resistant Bacteroides \= \____

thetaiotamicron

T/F: K. pneumoniae can grow on BBE.

T

T/F: Enterococcus can grow on BBE

T

T/F: Fusobacterium mortiferum can grow on BBE

T

Fusobacterium necrophorum \=\> \___ disease

Lemierre's

T/F: Bacteroides ureolyticus grows on BBE

F

T/F: Bilophila wadsworthia grows on BBE

T

Fish eye colony \=\> \___ \___

Bilophila wadsworthia

Prevotella and porphyromonas: pathogens of \___, \___, and \___ infections.

oral; dental; bite

Root canal infection \=\> \___ or \___

Prevotella; Porphyromonas

Prevotella \___ and \___ are lipase positive.

intermedia; loescheii

Prevotella \___ is spot indole positive

intermedia

Most Porphyromonas are spot indole \___

positive

Lumpy jaw \=\>\___ \___

Actinomyces israelii

Sulfur granules \=\> \___ \___

Actinomyces israelii

Branching, non-sporeforming GPBs slightly resembling Nocardia \=\> \____ \____

Actinomyces israelii

Molar tooth colony appearance \=\> \___ \___

Actinomyces israelii

Foodborne illness from improperly cooked meat or gravy w/ enterotoxin production that causes persistent diarrhea \=\> Clostridium \___

perfringens

Gas gangrene \= Clostridium \___

perfringens

Clostridium \___ causes pseudomembranous colitis.

difficile