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Polarity
The property of molecules having a partial positive charge on one atom and a partial negative charge on another atom
Hydrogen bonds
The weak bonds formed between the positive hydrogen atom of one water molecule and the negative oxygen atom of another water molecule
Cohesion
The ability of water molecules to stick together allowing them to form a continuous stream
Surface tension
The property of water that allows small organisms to move along its surface and live around it as a habitat
Adhesion
The ability of water molecules to stick to other polar molecules allowing water to flow through narrow paths and against gravity
Capillary action
Movement that results when water flows through a narrow path without help from gravity
Solvent properties
The ability of water to dissolve substances that are charged or polar creating solutions
Hydration shell
The sphere of water molecules around a dissolved ion using opposite charges
Amphipathic
Having both a hydrophilic region and a hydrophobic region
Metabolism
The sum of all chemical reactions that occur in an organism facilitated by water as a medium
Buoyancy
The property of water that describes the upward force exerted by a fluid on an object immersed in it allows organisms to float in water
Viscosity
The stickiness of a fluid influenced by the internal friction created when the fluid moves
Thermal conductivity
The property of water which describes the rate at which heat passes through a substance with water being higher than that of air
High specific heat
The property of water that requires a large amount of energy to raise its temperature resulting in a stable environment
Origin of Water
The theory that water on Earth was delivered by colliding asteroids during the early years of the planet
Goldilocks Zone
The region around a star where conditions are just right for the presence of liquid water and the potential for extraterrestrial life
Abiogenesis
Spontaneous origin of cells from non-living sources
Catalysis
self-assembly
Miller-Urey's Experiment
Demonstrated abiogenesis
boiled vapors
added H2 CH4 NH3 removed O2
added electrical discharge
produced 20AAs
Significance of Vesicles
Separated "self" from environment
created unique internal chemistry
Significance of RNA
Self-replicating
can work as an enzyme
LUCA
Last Universal Common Ancestor
microbe that existed ~4 to ~4.5 BYA
Estimation of Dates
Using isotope ratios and genomic analysis
Cell Theory (Robert Hooke)
Cells are the smallest unit of life
all living organisms are composed of cells
cells come from pre-existing cells
Structures No Longer Considered Organelles
Cell wall Cytoskeleton Cytoplasm
Universal Structures (3)
Plasma Membrane DNA Ribosomes
Plasma Membrane (Universal Structure)
Structure: phospholipid bilayer with embedded proteins and cholesterol Function: Semi-permeable barrier that separates internal/external environment
controls entry/exit of substance
DNA (Universal Structure)
Structure: double helix made of nucleotides Function: contains instructions for all cell functions specifically making proteins
Ribosomes (Universal Structure)
Structure: 2 subunits (large and small) made of rRNA and proteins no membrane two different sizes Function: Protein synthesis
70S vs. 80S
Prokaryotes have 70S Eukaryotes have 80S (larger)
Svedberg units (Ribosomes)
Measurement of the relative size of cell parts through sedimentation during centrifugation
Free vs Bound Ribosomes
Free ribosomes make proteins to be used in cytosol (In Cell) Bound ribosomes make secretory proteins like hormones ribosomes structurally identical and interchangeable every ribosome initially free then when needs to make secretory protein becomes bound to ER
Annotations for Prokaryote Structure
nucleoid naked DNA 70S ribosomes cytoplasm plasma membrane cell wall (thicker) pili/cilia flagella shape is more rod-shaped length 2x width
Nucleoid (Prokaryotes only)
Area where circular DNA is found
Plasmids (Prokaryotes only)
Extra circular DNA w/ additional info
Mesosomes (Prokaryotes only)
Infoldings that increase surface area and act as sites for cellular respiration
Cell Wall (Prokaryote)
Made of peptidoglycan protects cell maintains shape prevents bursting (prokaryote cell wants to be a bit turgid)
Flagella
Long slender extensions used for movement
Cilia
Hair-like sticks to surfaces used in bacterial conjugation
Nucleus
Structure: nuclear envelope is double membraned (2 phospholipid bilayers) with pores
contains nucleolus Function: stores genetic info as chromatin (DNA + Histones)
Nucleolus (Both)
Region in nucleus for ribosome synthesis (proteins for ribosomes synthesized outside of nucleus travel in to join w/ rRNA)
Benefits of Double Nuclear Membrane
Hydrophobic core is never exposed to water (if punctured it seals itself) transportation of materials in & out of nucleus
allows membrane break down and chromosomes to move to opposite poles of the cell
Rough Endoplasmic Reticulum (rER) (Both)
Structure: made of cisternae has 80S ribosomes attached Function: folds/packages secretory proteins (typically hormones)
Cisternae
Flattened membrane sacs (folds)
Smooth Endoplasmic Reticulum (sER) (Both)
Structure: made of branched tubular membranes
NO RIBOSOMES Function: makes lipids (phospholipids/hormones) detoxifies drugs stores calcium ions for muscles
Golgi Apparatus (Both)
Structure: consists of cisternae Function: receives vesicles from the rER (cis face)
modifies and ships most to plasma membrane for secretion (trans face) cis face always faces the nucleus
Vesicle Transport Model of Golgi Apparatus
Cisternae remains static vesicles move proteins between them
Cisternal Maturation Model of Golgi Apparatus
Vesicles from rER coalesce to from new cisternae on cis side which matures and moves to trans side which then breaks into vesicles
Lysosomes (Only animal cells)
Structure: membrane sacs filled with hydrolytic enzymes from Golgi Function: digests food organelles and sometimes entire cell (apoptosis)
Mitochondria (Both)
Structure: Double membrane like nucleus (outer membrane is smoother inner contains cristae)
matrix (fluid) inside
has own DNA and ribosomes makes own proteins Function: cellular respiration makes ATP
Cristae
Infoldings of the inner membrane of a mitochondrion that houses the electon transport chain for synthesis of ATP
Matrix
Center compartment of the mitochondrion that contains dissolved enzymes in fluid
Vacuoles (Vesicles) (Both)
Structure: single membraned sack filled with fluid contains dissolved materials Function: store food (animal cells) store poison pigments (plant cells) expel water (contractile vacuole in paramecium)
Central Vacuole (Only plant cells)
Found only in plant cells stores water pigments poison and maintains hydrostatic pressure
Vacuole vs. Vesicle
Large vs. small
permanent vs. temporary
Adaptations of Vesicles
Moves contents within
moves membrane or proteins that makeup vesicles
Clathrin
Three-legged protein that positions itself on the surface of membranes which then forms vesicles
Microtubules
Structure: made of tubulin protein Function: found in mitotic spindle fibers
cilia/flagella
moves organelles within cell (ex. vesicles)
Microfilaments
Structure: made of actin protein Function: cytoplasmic streaming (when you circulate cytosol to transport food/enzymes)
muscle contraction
helps animal cells maintain shape
Centrosome (Only animal cells)
Structure: contains 2 paired centrioles
each centriole made of 9 triplet microtubules Function: used as spindle fibers in mitosis/meiosis
Chloroplast (Only plant cells)
Structure: double membrane
contains stacks of thylakoids inside (in stacks called granum) Function: photosynthesis
makes glucose stored as starch grains
Cell Wall (composition is different) - no longer considered organelles
Structure: rigid outer layer made of cellulose Function: provides support protection prevents excess water uptake lets plant cell remain upright
What to label in microscope images
nucleus CHR (look for dark area) ribosomes rER/sER Golgi mitochondria chloroplast cell wall plasma membrane vacuole
What to annotate in animal cell drawing
nucleus double membrane w/ pores
mitochondria double membrane 1/2 nucleus size
Golgi apparatus w/ vesicles to and from
ER interconnected membrane (sER and rER)
ribosomes 80S
cytosol (NOT CYTOPLASM)
cell membrane one line
What to annotate in plant cell drawing
nucleus mitochondria golgi rER sER ribosomes cytosol (same as animal cell)
chloroplast two membranes stacks of disks (granum)
vacuole large takes up most space
tonoplast (membrane which surrounds vacuole)
cell membrane one line