The basic steps of an enzyme-catalyzed reaction are:
substrate and product combine, forming an S-P complex. The reaction occurs, enzymes are released, and the unchanged substrate is released and recycled.
substrate and enzyme combine, forming an E-S complex. The reaction occurs, products are released, and the unchanged enzyme is released and recycled.
substrate, enzyme, and product combine, forming an S-E-P complex. The reaction occurs, enzymes are released, and the unchanged enzyme is released and recycled.
two substrates combine, forming a third, which splits to yield two different products.
enzyme and product combine, forming an E-P complex. The reaction reverses, enzymes are released, and the unchanged substrate is released and recycled.