CLIBAC MIDTERMS LONG EXAM

Nonmotile

An isolate may be suspected to be B. anthracis if it is:

Urethral swab

What is the optimal specimen to collect for the diagnosis of gonorrhea by culture in males?

Erysipelothrix rhusiopathiae

What organism?

Characteristics:

Nonhemolytic on SBA
Gram-positive bacilli, no spores
Catalase negative
H2S production positive
Growth in gelatin resembled a test-tube brush

A methylene blue simple stain to test for bacteria having metachromatic granules and coryneform arrangement

A patient is observed to have a severe upper respiratory tract with a loose membranous tissue covering the back of the throat. What is the test to be done?

is arranged in a coryneform arrangement, with cells in V- and L-shaped patterns

Cause of diphtheria, Corynebacterium diphtheriae

Inhibits protein synthesis

The cytotoxin of Corynebacterium diphtheriae

Phenyl-ethyl alcohol agar plate

John is a resident on call at the resident room. His male patient has a urethral purulent discharge and he suspects gonorrhea.

Before collecting the specimen he requests a:

Many neutrophils and gram-positive cocci in pairs

The microbiology laboratory receives his patient's specimen and performs a Gram stain on it. He expects the lab will report the finding of:

Lysine decarboxylase

Colonies of Neisseria turn purple when a redox dye is applied. The color change is indicative of the enzyme:

Fastidious, blood factors

Haemophilus influenzae is \____ and requires special \____ for growth.

Undulant fever

Human brucellosis is also known as:

Tick bite

Francisella tularensis has which portal of entry?

Its cell membrane

The major virulence factor of Haemophilus influenzae type b is:

Chancroid

The following are zoonoses except for:

Placentas

Brucellosis is spread to humans by human contact with all but which of the following?

A pseudomembrane in the throat

What is the cardinal manifestation of human brucellosis?

Ticks and biting flies

Chief reservoir of Francisella tularensis

NAD or NADP

What is the V factor that is required by Haemophilus for growth?

Flu

What disease is caused by Haemophilus influenzae?

Cell wall lipopolysaccharide

What is the O antigen of Enterobacteriaciae?

Capsular, somatic, flagellar

Escherichia coli displays which antigens?

Klebsiella pneumoniae

The vast majority of clinical isolates of Klebsiella are:

Heat labile

The K antigens of the family Enterobacteriaciae are:

Paroxysmal coughing

A classic symptom of pertussis is:

alvei

The single species in the genus Hafnia is:

Shigella

Which of the following is NOT an opportunistic enteric bacterium?

sakazakii

Production of a yellow pigment is a characteristic of which species of Enterobacter?

MacConkey agar

Which of the following media would aid the technologist in determining if gram negative bacilli were lactose fermenters?

Neisseria gonorrhoeae

Characteristics:

Glucose (+)
Maltose, lactose, sucrose (-)

This organism is more likely to be:

Vancomycin-colistin-nystatin

A selective media for the isolation of gonococcus may prepared by adding which of the following antibiotic combos to the chocolate agar?

Cervix

Male: gonorrhea as Female gonorrhea: ?

Nocardia

Frosted glass colonies: B. cereus as Breadcrumb colonies: ?

Corynebacterium diphtheriae

Sulfur granules: Nocardia as Babes-Ernst granules: ?

Anisomycin

Modified Thayer-Martin: Nystatin as Martin-Lews: ?

Club-shaped

Streptococcus pneumoniae: lancet-shaped as Corynebacterium diphtheriae: ?

Listeria monocytogenes

Test tube brush pattern: Erysipelothrix rhusiopathiae as Tumbling motility: ?

HBT agar

Corynebacterium diphtheriae: CTBA as Gardnerella vaginalis: ?

Kidney-bean shaped

Bacillus: bamboo-shaped as Neisseria: ?

Listeria monocytogenes

Arrow-head hemolysis: Streptococcus agalactiae as Block-type hemolysis: ?

Food poisoning

Bacillus anthracis: Anthrax as Bacillus cereus: ?

Nocardia brasiliensis

Pulmonary infection: Nocardia asteroides as Cutaneous infection: ?

Tissue and pus

Gardnerella vaginalis: Vaginal discharge as Nocardia: ?

Bacterial vaginosis

Streptococcus pyogenes: Pharyngitis as Gardnerella vaginalis: ?

Corynebacterium diphtheriae

White to gray colonies on SBA: Staphylococcus epidermidis as Black or brown colonies on CTBA: ?

Vegetables

Emetic food poisoning: fried rice as diarrheal food poisoning:

Lysine Iron agar

bromocresol purple

Triple sugar iron

Phenol red

Hektoen Enteric Agar

Bromthymol blue

MacConkey agar

Neutral red

Hydrogen sulfide

Sodium thiosulfate

Enterohemorrhagic Escherichia coli

hemolytic uremic syndrome

Legionella pneumophila

Pontiac fever

Haemophilus aegyptius

pink eye

Haemophilus ducreyi

Soft chancre

Enterotoxigenic Escherichia coli

Watery diarrhea

E.coli 0157:H7

Sorbitol-MacConkey agar

Bordetella

Bordet-Gengou agar

Haemophilus aegyptius

CHOC with 1% IsoVitalex

Legionella

BCYE w/ L-cysteine

Salmonella

HE agar

Listeria monocytogenes

Stillborn in births
Meningitis in newborns
CNS infections and endocarditis

Bordetella pertussis

Regan-Lowe medium
Fat bacilli
Pertactin

Erysipelothrix rhusiopathiae

H2S (+)
Non motile
Test tube brush pattern in gelatin stab

Neisseria gonorrhoeae

Acute urethritis
Ophthalmia neonatorum
Pelvic inflammatory disease

Proteus mirabilis

Swarming colonies
Lysine iron agar: R/A
UTI

Bacillus anthracis

Woolsorter's disease
Bloody diarrhea
Black eschar

Corynebacterium diphtheriae

Catalase (+)
Gram (+) rod
Nonmotile
Black colony with brown halo in CTBA

Haemophilus ducreyi

Chancroid
Nairobi biplate medium
School of fish

Nonmotile
Bloody diarrhea
Infective dose

Haemophilus influenzae

Mousy odor colonies
Encapsulated
Meningitis

Arcanobacterium

Pitting of agar
Reverse CAMP reaction
Phospholipase D

Bordatella pertussis

Undulant fever
Category B bioterrorism agent
Chronic stage

Listeria monocytogenes

Gram (+) rod
Motile
Hippurate hydrolysis (+)
CAMP test (+): block hemolysis

Neisseria meningitidis

Purulent arthritis
Meningitis
Meningococcemia

Pasteurella multocida

Feline bites
Safety pin
Ovoid

Corynebacterium

Tough gray to white pseudomembrane in pharynx
Non healing skin ulcers

Escherichia coli

Citrate (-)
TSI: A/A + G
LIA: K/K or -/+

Gardnerella vaginalis

Clue cells
Normal flora of female genitalia tract
Gram (+) rods

Bacillus anthracis

Gram (+) rod
Aerobic spore-former
Subterminal spores
Ground glass colonies

Corynebacterium ulcerans

Mastitis in cattle
Skin ulcers in humans
Brown halo in CTBA
Coryneform
Urease (+)

Nocardia

Branching
Gram (+) rod
Weakly-acid fast
Breadcrumb colonies

Shigella dysenteriae

Lactose fermenter
Rhinoscleroma
Nosocomial agent

Legionella pneumophila

Hot water systems
DFA test
Pneumonia

Salmonella typhi

Non-lactose fermenter
H2S (+)
Vi antigen

Moraxella catarrhalis

Hockey puck colonies
Asaccharolytic
Otitis media and sinusitis

E. coli O157:H7

EHEC strain that causes hemolytic uremic syndrome

ETEC

E. coli strain that causes dysentery

Pasteurella

What bacteria is positive for bipolar staining and has a safety pin appearance?

Bordatella

Small, fat bacilli or coccobacilli with intense peripheral yellow-green fluorescence and darker centers

Serologic culture

Most important test for Legionnaire's disease

Negative

Result of E. coli in citrate test

Haemophilus influenzae

Haemophilus species that has a "mousy" or "bleachlike odor"

Hafnia

Enteric species present in beer wort of breweries

Vi

Capsular antigen of Salmonella is designated as

Somatic antigen

Another name for O antigen

Prodigiosin

Red pigment produced by Serratia

EMB agar

E. coli produces greenish metallic sheen on what agar?

E. coli

most common cause of traveler's diarrhea

H. ducreyi

Of all species that require the V factor, what is the only one that is oxidase negative?

Bordet-Gengou medium

Culture medium used for determining the hemolytic pattern of Bordatella