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What are the most common viruses causing infections of the GI tract?
Hep A
Rotavirus
Adenovirus
Norovirus
What species cause intoxications of GI tract?
Clostridium botulinum
Clostridium perfringens
S. Aureus
Bacillus cereus
What species do not causes food borne intoxication?
Viruses and parasites
Determinants of Salmonella pathogenicity:
Endotoxin = LPS
Invasion proteins
What is the function of invasion proteins?
Allow entry of salmonella into intestinal epithelial cells
What species cause salmonellosis?
S.enteritidis
S.typhimurium
Features of salmonellosis?
Causes diarrhea, abdominal pain
Self limited
Lasts 2-5 days
Causes dehydration and electrolyte imbalance
Prevention of typhoid fever:
Adequate sewage disposal, sanitation measures, personal hygiene
Vaccines
What is Shiga toxin?
Is a cytotoxic exotoxin produced by S.dysenteriae type 1 or 2
Inhibits protein synthesis in humans, causing death of mucosal cells
What does Shiga toxin cause?
Hemolytic-uremic syndrome (disseminated endothelial damage leading to intravascular clotting, hemolysis and renal failure)
What is used to detect s.dysenteriae type 1 in stool?
An enzyme immunoassay for Stx
What antibiotics are used to treat shigellosis in severe cases?
Ampicillin
Amoxicillin
Trimethoprim-sulfametgoxasole
Ciprofloxacin
Azithromycin
Treatment of urinary tract infections caused by e.coli?
Fluoroquinolones
Sulphonamides
Treatment of gastroenteritis caused by e.coli?
Rehydration
TMP/SXT
Beta lactams
Characteristic features of vibrio:
Gram negative, comma shaped rods with polar flagella
Oxidase positive
Aerobic
Alkaliphile
Virulence factor of v.cholera?
Choleragen (enterotoxin)
Diagnostics of V.cholerae?
Gold standard = culture of stool specimen
TCBS - large yellow colonies
Microscopic appearance of Campylobacter:
Gram neg rods (gulls’ wing shape rods)
Flagella
Cause of infections by campylobacter?
Contaminated poultry (c.jejuni)
Water
Milk
Contact with infected animals
Complications associated with campylobacteriosis?
Gueillain-Barre syndrome
Reactive arthritis