coochie and ear cytology

Define estrous cycle

Puberty

Stages of estrous

Proestrus

Estrus

Metestrus/ Diestrus

Anestrus

Proestrus symptoms / duration

Swollen vulva

Bloody vaginal discharge

Becomes a tease

Urine marking

Average 7-10 days

Range 4-25 days

Estrus symptoms / duration

Vulva is less swollen

Less bloody discharge

Straw coloured discharge

Urine marking

On the hunt for a lover, and the guys be noticing and wanting to engage. Bow chick a wow wow time

Average 3-10 days Range

Range 2-15 days

Metestrus / diestrus symptoms / duration

Normal cooch

Mucous to dark brown color initially

May have morning sickness for a few days if prego

Can undergo false pregnancy

70-80 days

Anestrus symptoms / duration

Normal cooch

Period if inactivity between end of diestrus and the onset of the next Proestrus

4-5 months

How do you determine ovulation

Vag cytology

LH determination

Serum progesterone determination

When would you get increased estrogen results on a vag cytology

Bloody vaginal fluid (diapedesis)

Epithelial cell proliferation and diffeeentiation (thickening of epithelium or keratinization of cells)

Are vag cytologies usually done in dogs to stage the estrous cycle

Yes

How to take a vag smear

Moisten cotton swab

Use speculum

Touch vaginal wall with swab, do not scrape

Avoid collecting cells from vestibule or clitoral fossa

Roll swab on slide when removed

Classifications of vaginal epithelial cells

Cornified cells: dead/ dying cells - Superficial cells, Anuclear cells

Noncornifiee cells : living cells are divided up into -intermediate cells, parabasal cells, basal cells (rare)

Describe as epithelial cells become Cornified (keratinized) cells

The cell size increases

Nucleus changes from oval to faded, broken, pyknotic as the cell dies it then disappears

Cytoplasm changes from dark blue to light blue purple as the cell dies

2 other variety of parabasal cells

Foam cells

Metestral cells

On cytology on Proestrus what would you see

High RBC

Incr Cornified cells

On cytology on estrus what would you see

Few or no RBCs/wbcs

Nearly 100% Cornified cells

On cytology on Metestrus / diestrus what would you see

High wbc

Metestrum / foam cells

30% Cornified cells

On cytology on Anestrus what would you see

Few wbcs

30% Cornified cells

what cells are these

Anuclear

Superficial

Intermediate

Parabasal

What cell dis

Foam cell

What cell dis

Metestral cells

What estrous stage is this

Proestrus

What estrous stage is this

Estrus

What estrous stage is this

Metestrus/ diestrus

What estrous stage is this

Anestrus

What to look for on 10x for ear cytology

Epithelial cells

Mites/ova

Tumor cells

Fungal hyphae

What to look for on 40 x and 100x for ear cytology

Organism type and number (rods, cocci, yeast

WBC (= infection / inflammation)

RBC (=hemorrhage with trauma, infection, tumor)

How to grade ear cytology

What dis

yeast

What dis

Keratinocyte with melanin granules

Who’s all at this party

rods

Cocci

Neutrophils

Who’s at this party

Melanin granules

Nuclear streaming

Cocci

Neutrophils